SRX15932888 GSM6257856_r1 SRP384053 PRJNA851453 SRS13621035 GSM6257856 GSM6257856 ChIP-Seq GENOMIC ChIP All steps ares performed at 4˚C. The cultures were centrifuged at 4000 rpm for 10 mins at 4˚C, washed twice with 10 ml ice-cold PBS, and the cell pellet was store at -80 ˚C for at least 24 h. Cell pellet was thawed and resuspended in 2ml ChIP lysis buffer followed by incubation for ~1 h. After incubation, the samples were then sonicated for ~9-13 mins (10 sec on/10 sec off, 30% amplitude) to yield DNA fragments of ~300-500 bps using Q500 sonicator (500W, 110V with four probe horn assembly, QSONICA, Newtown, CT). Lysate was then centrifuged at 4000 rpm for 10 minutes and the cleared supernatant was kept for further processing. 20 µ Illumina NovaSeq 6000