SRX17426742 Sample_Fo5-1 SRP395636 bp0 RNA was extracted from Fo5 tumors treated in vivo with tucatinib 50 mg/kg or vehicle (methyl cellulose 0.5%) for 10 days using TRIzol Reagent with the PureLink RNA Mini Kit (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA). Quantity and integrity of the total RNA was checked using a TapeStation 2200 (Agilent Technologies) and 500 ng used for library preparation according to standard protocols (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina and NEBNext Poly(A) mRNA Magnetic Isolation Module, NEB). Indexed libraries were pooled and sequenced on a NextSeq500 flowcell (Illumina) to generate 25-50 million paired-end 75 bp reads per sample. SRS14982218 Sample_Fo5-1 Sample_Fo5-1 RNA-Seq TRANSCRIPTOMIC PCR NextSeq 500 SRX17426743 Sample_Fo5-2 SRP395636 bp0 RNA was extracted from Fo5 tumors treated in vivo with tucatinib 50 mg/kg or vehicle (methyl cellulose 0.5%) for 10 days using TRIzol Reagent with the PureLink RNA Mini Kit (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA). Quantity and integrity of the total RNA was checked using a TapeStation 2200 (Agilent Technologies) and 500 ng used for library preparation according to standard protocols (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina and NEBNext Poly(A) mRNA Magnetic Isolation Module, NEB). Indexed libraries were pooled and sequenced on a NextSeq500 flowcell (Illumina) to generate 25-50 million paired-end 75 bp reads per sample. SRS14982219 Sample_Fo5-2 Sample_Fo5-2 RNA-Seq TRANSCRIPTOMIC PCR NextSeq 500 SRX17426744 Sample_Fo5-3 SRP395636 bp0 RNA was extracted from Fo5 tumors treated in vivo with tucatinib 50 mg/kg or vehicle (methyl cellulose 0.5%) for 10 days using TRIzol Reagent with the PureLink RNA Mini Kit (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA). Quantity and integrity of the total RNA was checked using a TapeStation 2200 (Agilent Technologies) and 500 ng used for library preparation according to standard protocols (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina and NEBNext Poly(A) mRNA Magnetic Isolation Module, NEB). Indexed libraries were pooled and sequenced on a NextSeq500 flowcell (Illumina) to generate 25-50 million paired-end 75 bp reads per sample. SRS14982220 Sample_Fo5-3 Sample_Fo5-3 RNA-Seq TRANSCRIPTOMIC PCR NextSeq 500 SRX17426745 Sample_Fo5-4 SRP395636 bp0 RNA was extracted from Fo5 tumors treated in vivo with tucatinib 50 mg/kg or vehicle (methyl cellulose 0.5%) for 10 days using TRIzol Reagent with the PureLink RNA Mini Kit (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA). Quantity and integrity of the total RNA was checked using a TapeStation 2200 (Agilent Technologies) and 500 ng used for library preparation according to standard protocols (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina and NEBNext Poly(A) mRNA Magnetic Isolation Module, NEB). Indexed libraries were pooled and sequenced on a NextSeq500 flowcell (Illumina) to generate 25-50 million paired-end 75 bp reads per sample. SRS14982221 Sample_Fo5-4 Sample_Fo5-4 RNA-Seq TRANSCRIPTOMIC PCR NextSeq 500 SRX17426746 Sample_Fo5-5 SRP395636 bp0 RNA was extracted from Fo5 tumors treated in vivo with tucatinib 50 mg/kg or vehicle (methyl cellulose 0.5%) for 10 days using TRIzol Reagent with the PureLink RNA Mini Kit (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA). Quantity and integrity of the total RNA was checked using a TapeStation 2200 (Agilent Technologies) and 500 ng used for library preparation according to standard protocols (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina and NEBNext Poly(A) mRNA Magnetic Isolation Module, NEB). Indexed libraries were pooled and sequenced on a NextSeq500 flowcell (Illumina) to generate 25-50 million paired-end 75 bp reads per sample. SRS14982222 Sample_Fo5-5 Sample_Fo5-5 RNA-Seq TRANSCRIPTOMIC PCR NextSeq 500 SRX17426747 Sample_Fo5-Tuc-01 SRP395636 bp0 RNA was extracted from Fo5 tumors treated in vivo with tucatinib 50 mg/kg or vehicle (methyl cellulose 0.5%) for 10 days using TRIzol Reagent with the PureLink RNA Mini Kit (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA). Quantity and integrity of the total RNA was checked using a TapeStation 2200 (Agilent Technologies) and 500 ng used for library preparation according to standard protocols (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina and NEBNext Poly(A) mRNA Magnetic Isolation Module, NEB). Indexed libraries were pooled and sequenced on a NextSeq500 flowcell (Illumina) to generate 25-50 million paired-end 75 bp reads per sample. SRS14982223 Sample_Fo5-Tuc-01 Sample_Fo5-Tuc-01 RNA-Seq TRANSCRIPTOMIC PCR NextSeq 500 SRX17426748 Sample_Fo5-Tuc-02 SRP395636 bp0 RNA was extracted from Fo5 tumors treated in vivo with tucatinib 50 mg/kg or vehicle (methyl cellulose 0.5%) for 10 days using TRIzol Reagent with the PureLink RNA Mini Kit (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA). Quantity and integrity of the total RNA was checked using a TapeStation 2200 (Agilent Technologies) and 500 ng used for library preparation according to standard protocols (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina and NEBNext Poly(A) mRNA Magnetic Isolation Module, NEB). Indexed libraries were pooled and sequenced on a NextSeq500 flowcell (Illumina) to generate 25-50 million paired-end 75 bp reads per sample. SRS14982224 Sample_Fo5-Tuc-02 Sample_Fo5-Tuc-02 RNA-Seq TRANSCRIPTOMIC PCR NextSeq 500 SRX17426749 Sample_Fo5-Tuc-03 SRP395636 bp0 RNA was extracted from Fo5 tumors treated in vivo with tucatinib 50 mg/kg or vehicle (methyl cellulose 0.5%) for 10 days using TRIzol Reagent with the PureLink RNA Mini Kit (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA). Quantity and integrity of the total RNA was checked using a TapeStation 2200 (Agilent Technologies) and 500 ng used for library preparation according to standard protocols (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina and NEBNext Poly(A) mRNA Magnetic Isolation Module, NEB). Indexed libraries were pooled and sequenced on a NextSeq500 flowcell (Illumina) to generate 25-50 million paired-end 75 bp reads per sample. SRS14982225 Sample_Fo5-Tuc-03 Sample_Fo5-Tuc-03 RNA-Seq TRANSCRIPTOMIC PCR NextSeq 500 SRX17426750 Sample_Fo5-Tuc-04 SRP395636 bp0 RNA was extracted from Fo5 tumors treated in vivo with tucatinib 50 mg/kg or vehicle (methyl cellulose 0.5%) for 10 days using TRIzol Reagent with the PureLink RNA Mini Kit (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA). Quantity and integrity of the total RNA was checked using a TapeStation 2200 (Agilent Technologies) and 500 ng used for library preparation according to standard protocols (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina and NEBNext Poly(A) mRNA Magnetic Isolation Module, NEB). Indexed libraries were pooled and sequenced on a NextSeq500 flowcell (Illumina) to generate 25-50 million paired-end 75 bp reads per sample. SRS14982226 Sample_Fo5-Tuc-04 Sample_Fo5-Tuc-04 RNA-Seq TRANSCRIPTOMIC PCR NextSeq 500