SRX17776195 GSM6613212_r1 SRP400673 PRJNA886413 SRS15302458 GSM6613212 GSM6613212 RNA-Seq TRANSCRIPTOMIC cDNA Brains were removed and the cortexes were dissected, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Total RNA is enriched by oligo (dT) magnetic beads (rRNA removed); 2. RNA-seq library preparation using KAPA Stranded RNA-Seq Library Prep Kit (Illumina), which incorporates dUTP into the second cDNA strand and renders the RNA-seq library strand-specific. The completed libraries were qualified with Agilent 2100 Bioanalyzer and quantified by absolute quantification qPCR method. To sequence the libraries on the Illumina NovaSeq 6000 instrument, the barcoded libraries were mixed, denatured to single stranded DNA in NaOH, captured on Illumina flow cell, amplified in situ, and subsequently sequenced for 150 cycles for both ends on Illumina NovaSeq 6000 instrument. The completed libraries were qualified on Agilent 2100 Bioanalyzer for concentration, fragment size distribution between 400 ∼ 600 bp, and adapter dimer contamination. The amount was determined by absolute quantification qPCR method. The barcoded libraries were mixed in equal amounts and used for sequencing on the instrument. Illumina NovaSeq 6000 SRX17776196 GSM6613213_r1 SRP400673 PRJNA886413 SRS15302460 GSM6613213 GSM6613213 RNA-Seq TRANSCRIPTOMIC cDNA Brains were removed and the cortexes were dissected, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Total RNA is enriched by oligo (dT) magnetic beads (rRNA removed); 2. RNA-seq library preparation using KAPA Stranded RNA-Seq Library Prep Kit (Illumina), which incorporates dUTP into the second cDNA strand and renders the RNA-seq library strand-specific. The completed libraries were qualified with Agilent 2100 Bioanalyzer and quantified by absolute quantification qPCR method. To sequence the libraries on the Illumina NovaSeq 6000 instrument, the barcoded libraries were mixed, denatured to single stranded DNA in NaOH, captured on Illumina flow cell, amplified in situ, and subsequently sequenced for 150 cycles for both ends on Illumina NovaSeq 6000 instrument. The completed libraries were qualified on Agilent 2100 Bioanalyzer for concentration, fragment size distribution between 400 ∼ 600 bp, and adapter dimer contamination. The amount was determined by absolute quantification qPCR method. The barcoded libraries were mixed in equal amounts and used for sequencing on the instrument. Illumina NovaSeq 6000 SRX17776197 GSM6613214_r1 SRP400673 PRJNA886413 SRS15302459 GSM6613214 GSM6613214 RNA-Seq TRANSCRIPTOMIC cDNA Brains were removed and the cortexes were dissected, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Total RNA is enriched by oligo (dT) magnetic beads (rRNA removed); 2. RNA-seq library preparation using KAPA Stranded RNA-Seq Library Prep Kit (Illumina), which incorporates dUTP into the second cDNA strand and renders the RNA-seq library strand-specific. The completed libraries were qualified with Agilent 2100 Bioanalyzer and quantified by absolute quantification qPCR method. To sequence the libraries on the Illumina NovaSeq 6000 instrument, the barcoded libraries were mixed, denatured to single stranded DNA in NaOH, captured on Illumina flow cell, amplified in situ, and subsequently sequenced for 150 cycles for both ends on Illumina NovaSeq 6000 instrument. The completed libraries were qualified on Agilent 2100 Bioanalyzer for concentration, fragment size distribution between 400 ∼ 600 bp, and adapter dimer contamination. The amount was determined by absolute quantification qPCR method. The barcoded libraries were mixed in equal amounts and used for sequencing on the instrument. Illumina NovaSeq 6000 SRX17776198 GSM6613215_r1 SRP400673 PRJNA886413 SRS15302461 GSM6613215 GSM6613215 RNA-Seq TRANSCRIPTOMIC cDNA Brains were removed and the cortexes were dissected, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Total RNA is enriched by oligo (dT) magnetic beads (rRNA removed); 2. RNA-seq library preparation using KAPA Stranded RNA-Seq Library Prep Kit (Illumina), which incorporates dUTP into the second cDNA strand and renders the RNA-seq library strand-specific. The completed libraries were qualified with Agilent 2100 Bioanalyzer and quantified by absolute quantification qPCR method. To sequence the libraries on the Illumina NovaSeq 6000 instrument, the barcoded libraries were mixed, denatured to single stranded DNA in NaOH, captured on Illumina flow cell, amplified in situ, and subsequently sequenced for 150 cycles for both ends on Illumina NovaSeq 6000 instrument. The completed libraries were qualified on Agilent 2100 Bioanalyzer for concentration, fragment size distribution between 400 ∼ 600 bp, and adapter dimer contamination. The amount was determined by absolute quantification qPCR method. The barcoded libraries were mixed in equal amounts and used for sequencing on the instrument. Illumina NovaSeq 6000 SRX17776199 GSM6613216_r1 SRP400673 PRJNA886413 SRS15302462 GSM6613216 GSM6613216 RNA-Seq TRANSCRIPTOMIC cDNA Brains were removed and the cortexes were dissected, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Total RNA is enriched by oligo (dT) magnetic beads (rRNA removed); 2. RNA-seq library preparation using KAPA Stranded RNA-Seq Library Prep Kit (Illumina), which incorporates dUTP into the second cDNA strand and renders the RNA-seq library strand-specific. The completed libraries were qualified with Agilent 2100 Bioanalyzer and quantified by absolute quantification qPCR method. To sequence the libraries on the Illumina NovaSeq 6000 instrument, the barcoded libraries were mixed, denatured to single stranded DNA in NaOH, captured on Illumina flow cell, amplified in situ, and subsequently sequenced for 150 cycles for both ends on Illumina NovaSeq 6000 instrument. The completed libraries were qualified on Agilent 2100 Bioanalyzer for concentration, fragment size distribution between 400 ∼ 600 bp, and adapter dimer contamination. The amount was determined by absolute quantification qPCR method. The barcoded libraries were mixed in equal amounts and used for sequencing on the instrument. Illumina NovaSeq 6000 SRX17776200 GSM6613217_r1 SRP400673 PRJNA886413 SRS15302463 GSM6613217 GSM6613217 RNA-Seq TRANSCRIPTOMIC cDNA Brains were removed and the cortexes were dissected, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Total RNA is enriched by oligo (dT) magnetic beads (rRNA removed); 2. RNA-seq library preparation using KAPA Stranded RNA-Seq Library Prep Kit (Illumina), which incorporates dUTP into the second cDNA strand and renders the RNA-seq library strand-specific. The completed libraries were qualified with Agilent 2100 Bioanalyzer and quantified by absolute quantification qPCR method. To sequence the libraries on the Illumina NovaSeq 6000 instrument, the barcoded libraries were mixed, denatured to single stranded DNA in NaOH, captured on Illumina flow cell, amplified in situ, and subsequently sequenced for 150 cycles for both ends on Illumina NovaSeq 6000 instrument. The completed libraries were qualified on Agilent 2100 Bioanalyzer for concentration, fragment size distribution between 400 ∼ 600 bp, and adapter dimer contamination. The amount was determined by absolute quantification qPCR method. The barcoded libraries were mixed in equal amounts and used for sequencing on the instrument. Illumina NovaSeq 6000 SRX17776201 GSM6613218_r1 SRP400673 PRJNA886413 SRS15302464 GSM6613218 GSM6613218 RNA-Seq TRANSCRIPTOMIC cDNA Brains were removed and the cortexes were dissected, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Total RNA is enriched by oligo (dT) magnetic beads (rRNA removed); 2. RNA-seq library preparation using KAPA Stranded RNA-Seq Library Prep Kit (Illumina), which incorporates dUTP into the second cDNA strand and renders the RNA-seq library strand-specific. The completed libraries were qualified with Agilent 2100 Bioanalyzer and quantified by absolute quantification qPCR method. To sequence the libraries on the Illumina NovaSeq 6000 instrument, the barcoded libraries were mixed, denatured to single stranded DNA in NaOH, captured on Illumina flow cell, amplified in situ, and subsequently sequenced for 150 cycles for both ends on Illumina NovaSeq 6000 instrument. The completed libraries were qualified on Agilent 2100 Bioanalyzer for concentration, fragment size distribution between 400 ∼ 600 bp, and adapter dimer contamination. The amount was determined by absolute quantification qPCR method. The barcoded libraries were mixed in equal amounts and used for sequencing on the instrument. Illumina NovaSeq 6000 SRX17776202 GSM6613219_r1 SRP400673 PRJNA886413 SRS15302465 GSM6613219 GSM6613219 RNA-Seq TRANSCRIPTOMIC cDNA Brains were removed and the cortexes were dissected, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Total RNA is enriched by oligo (dT) magnetic beads (rRNA removed); 2. RNA-seq library preparation using KAPA Stranded RNA-Seq Library Prep Kit (Illumina), which incorporates dUTP into the second cDNA strand and renders the RNA-seq library strand-specific. The completed libraries were qualified with Agilent 2100 Bioanalyzer and quantified by absolute quantification qPCR method. To sequence the libraries on the Illumina NovaSeq 6000 instrument, the barcoded libraries were mixed, denatured to single stranded DNA in NaOH, captured on Illumina flow cell, amplified in situ, and subsequently sequenced for 150 cycles for both ends on Illumina NovaSeq 6000 instrument. The completed libraries were qualified on Agilent 2100 Bioanalyzer for concentration, fragment size distribution between 400 ∼ 600 bp, and adapter dimer contamination. The amount was determined by absolute quantification qPCR method. The barcoded libraries were mixed in equal amounts and used for sequencing on the instrument. Illumina NovaSeq 6000 SRX17776203 GSM6613220_r1 SRP400673 PRJNA886413 SRS15302466 GSM6613220 GSM6613220 RNA-Seq TRANSCRIPTOMIC cDNA Brains were removed and the cortexes were dissected, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Total RNA is enriched by oligo (dT) magnetic beads (rRNA removed); 2. RNA-seq library preparation using KAPA Stranded RNA-Seq Library Prep Kit (Illumina), which incorporates dUTP into the second cDNA strand and renders the RNA-seq library strand-specific. The completed libraries were qualified with Agilent 2100 Bioanalyzer and quantified by absolute quantification qPCR method. To sequence the libraries on the Illumina NovaSeq 6000 instrument, the barcoded libraries were mixed, denatured to single stranded DNA in NaOH, captured on Illumina flow cell, amplified in situ, and subsequently sequenced for 150 cycles for both ends on Illumina NovaSeq 6000 instrument. The completed libraries were qualified on Agilent 2100 Bioanalyzer for concentration, fragment size distribution between 400 ∼ 600 bp, and adapter dimer contamination. The amount was determined by absolute quantification qPCR method. The barcoded libraries were mixed in equal amounts and used for sequencing on the instrument. Illumina NovaSeq 6000 SRX17776204 GSM6613221_r1 SRP400673 PRJNA886413 SRS15302467 GSM6613221 GSM6613221 RNA-Seq TRANSCRIPTOMIC cDNA Brains were removed and the cortexes were dissected, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Total RNA is enriched by oligo (dT) magnetic beads (rRNA removed); 2. RNA-seq library preparation using KAPA Stranded RNA-Seq Library Prep Kit (Illumina), which incorporates dUTP into the second cDNA strand and renders the RNA-seq library strand-specific. The completed libraries were qualified with Agilent 2100 Bioanalyzer and quantified by absolute quantification qPCR method. To sequence the libraries on the Illumina NovaSeq 6000 instrument, the barcoded libraries were mixed, denatured to single stranded DNA in NaOH, captured on Illumina flow cell, amplified in situ, and subsequently sequenced for 150 cycles for both ends on Illumina NovaSeq 6000 instrument. The completed libraries were qualified on Agilent 2100 Bioanalyzer for concentration, fragment size distribution between 400 ∼ 600 bp, and adapter dimer contamination. The amount was determined by absolute quantification qPCR method. The barcoded libraries were mixed in equal amounts and used for sequencing on the instrument. Illumina NovaSeq 6000 SRX17776205 GSM6613223_r1 SRP400673 PRJNA886413 SRS15302468 GSM6613223 GSM6613223 RNA-Seq TRANSCRIPTOMIC cDNA Brains were removed and the cortexes were dissected, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Total RNA is enriched by oligo (dT) magnetic beads (rRNA removed); 2. RNA-seq library preparation using KAPA Stranded RNA-Seq Library Prep Kit (Illumina), which incorporates dUTP into the second cDNA strand and renders the RNA-seq library strand-specific. The completed libraries were qualified with Agilent 2100 Bioanalyzer and quantified by absolute quantification qPCR method. To sequence the libraries on the Illumina NovaSeq 6000 instrument, the barcoded libraries were mixed, denatured to single stranded DNA in NaOH, captured on Illumina flow cell, amplified in situ, and subsequently sequenced for 150 cycles for both ends on Illumina NovaSeq 6000 instrument. The completed libraries were qualified on Agilent 2100 Bioanalyzer for concentration, fragment size distribution between 400 ∼ 600 bp, and adapter dimer contamination. The amount was determined by absolute quantification qPCR method. The barcoded libraries were mixed in equal amounts and used for sequencing on the instrument. Illumina NovaSeq 6000 SRX17776206 GSM6613222_r1 SRP400673 PRJNA886413 SRS15302469 GSM6613222 GSM6613222 RNA-Seq TRANSCRIPTOMIC cDNA Brains were removed and the cortexes were dissected, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Total RNA is enriched by oligo (dT) magnetic beads (rRNA removed); 2. RNA-seq library preparation using KAPA Stranded RNA-Seq Library Prep Kit (Illumina), which incorporates dUTP into the second cDNA strand and renders the RNA-seq library strand-specific. The completed libraries were qualified with Agilent 2100 Bioanalyzer and quantified by absolute quantification qPCR method. To sequence the libraries on the Illumina NovaSeq 6000 instrument, the barcoded libraries were mixed, denatured to single stranded DNA in NaOH, captured on Illumina flow cell, amplified in situ, and subsequently sequenced for 150 cycles for both ends on Illumina NovaSeq 6000 instrument. The completed libraries were qualified on Agilent 2100 Bioanalyzer for concentration, fragment size distribution between 400 ∼ 600 bp, and adapter dimer contamination. The amount was determined by absolute quantification qPCR method. The barcoded libraries were mixed in equal amounts and used for sequencing on the instrument. Illumina NovaSeq 6000 SRX17776207 GSM6613224_r1 SRP400673 PRJNA886413 SRS15302470 GSM6613224 GSM6613224 RNA-Seq TRANSCRIPTOMIC cDNA Brains were removed and the cortexes were dissected, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Total RNA is enriched by oligo (dT) magnetic beads (rRNA removed); 2. RNA-seq library preparation using KAPA Stranded RNA-Seq Library Prep Kit (Illumina), which incorporates dUTP into the second cDNA strand and renders the RNA-seq library strand-specific. The completed libraries were qualified with Agilent 2100 Bioanalyzer and quantified by absolute quantification qPCR method. To sequence the libraries on the Illumina NovaSeq 6000 instrument, the barcoded libraries were mixed, denatured to single stranded DNA in NaOH, captured on Illumina flow cell, amplified in situ, and subsequently sequenced for 150 cycles for both ends on Illumina NovaSeq 6000 instrument. The completed libraries were qualified on Agilent 2100 Bioanalyzer for concentration, fragment size distribution between 400 ∼ 600 bp, and adapter dimer contamination. The amount was determined by absolute quantification qPCR method. The barcoded libraries were mixed in equal amounts and used for sequencing on the instrument. Illumina NovaSeq 6000 SRX17776208 GSM6613225_r1 SRP400673 PRJNA886413 SRS15302471 GSM6613225 GSM6613225 RNA-Seq TRANSCRIPTOMIC cDNA Brains were removed and the cortexes were dissected, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Total RNA is enriched by oligo (dT) magnetic beads (rRNA removed); 2. RNA-seq library preparation using KAPA Stranded RNA-Seq Library Prep Kit (Illumina), which incorporates dUTP into the second cDNA strand and renders the RNA-seq library strand-specific. The completed libraries were qualified with Agilent 2100 Bioanalyzer and quantified by absolute quantification qPCR method. To sequence the libraries on the Illumina NovaSeq 6000 instrument, the barcoded libraries were mixed, denatured to single stranded DNA in NaOH, captured on Illumina flow cell, amplified in situ, and subsequently sequenced for 150 cycles for both ends on Illumina NovaSeq 6000 instrument. The completed libraries were qualified on Agilent 2100 Bioanalyzer for concentration, fragment size distribution between 400 ∼ 600 bp, and adapter dimer contamination. The amount was determined by absolute quantification qPCR method. The barcoded libraries were mixed in equal amounts and used for sequencing on the instrument. Illumina NovaSeq 6000 SRX17776209 GSM6613226_r1 SRP400673 PRJNA886413 SRS15302472 GSM6613226 GSM6613226 RNA-Seq TRANSCRIPTOMIC cDNA Brains were removed and the cortexes were dissected, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Total RNA is enriched by oligo (dT) magnetic beads (rRNA removed); 2. RNA-seq library preparation using KAPA Stranded RNA-Seq Library Prep Kit (Illumina), which incorporates dUTP into the second cDNA strand and renders the RNA-seq library strand-specific. The completed libraries were qualified with Agilent 2100 Bioanalyzer and quantified by absolute quantification qPCR method. To sequence the libraries on the Illumina NovaSeq 6000 instrument, the barcoded libraries were mixed, denatured to single stranded DNA in NaOH, captured on Illumina flow cell, amplified in situ, and subsequently sequenced for 150 cycles for both ends on Illumina NovaSeq 6000 instrument. The completed libraries were qualified on Agilent 2100 Bioanalyzer for concentration, fragment size distribution between 400 ∼ 600 bp, and adapter dimer contamination. The amount was determined by absolute quantification qPCR method. The barcoded libraries were mixed in equal amounts and used for sequencing on the instrument. Illumina NovaSeq 6000 SRX17776210 GSM6613227_r1 SRP400673 PRJNA886413 SRS15302473 GSM6613227 GSM6613227 RNA-Seq TRANSCRIPTOMIC cDNA Brains were removed and the cortexes were dissected, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Total RNA is enriched by oligo (dT) magnetic beads (rRNA removed); 2. RNA-seq library preparation using KAPA Stranded RNA-Seq Library Prep Kit (Illumina), which incorporates dUTP into the second cDNA strand and renders the RNA-seq library strand-specific. The completed libraries were qualified with Agilent 2100 Bioanalyzer and quantified by absolute quantification qPCR method. To sequence the libraries on the Illumina NovaSeq 6000 instrument, the barcoded libraries were mixed, denatured to single stranded DNA in NaOH, captured on Illumina flow cell, amplified in situ, and subsequently sequenced for 150 cycles for both ends on Illumina NovaSeq 6000 instrument. The completed libraries were qualified on Agilent 2100 Bioanalyzer for concentration, fragment size distribution between 400 ∼ 600 bp, and adapter dimer contamination. The amount was determined by absolute quantification qPCR method. The barcoded libraries were mixed in equal amounts and used for sequencing on the instrument. Illumina NovaSeq 6000 SRX17776211 GSM6613228_r1 SRP400673 PRJNA886413 SRS15302474 GSM6613228 GSM6613228 RNA-Seq TRANSCRIPTOMIC cDNA Brains were removed and the cortexes were dissected, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Total RNA is enriched by oligo (dT) magnetic beads (rRNA removed); 2. RNA-seq library preparation using KAPA Stranded RNA-Seq Library Prep Kit (Illumina), which incorporates dUTP into the second cDNA strand and renders the RNA-seq library strand-specific. The completed libraries were qualified with Agilent 2100 Bioanalyzer and quantified by absolute quantification qPCR method. To sequence the libraries on the Illumina NovaSeq 6000 instrument, the barcoded libraries were mixed, denatured to single stranded DNA in NaOH, captured on Illumina flow cell, amplified in situ, and subsequently sequenced for 150 cycles for both ends on Illumina NovaSeq 6000 instrument. The completed libraries were qualified on Agilent 2100 Bioanalyzer for concentration, fragment size distribution between 400 ∼ 600 bp, and adapter dimer contamination. The amount was determined by absolute quantification qPCR method. The barcoded libraries were mixed in equal amounts and used for sequencing on the instrument. Illumina NovaSeq 6000 SRX17776212 GSM6613229_r1 SRP400673 PRJNA886413 SRS15302475 GSM6613229 GSM6613229 RNA-Seq TRANSCRIPTOMIC cDNA Brains were removed and the cortexes were dissected, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Total RNA is enriched by oligo (dT) magnetic beads (rRNA removed); 2. RNA-seq library preparation using KAPA Stranded RNA-Seq Library Prep Kit (Illumina), which incorporates dUTP into the second cDNA strand and renders the RNA-seq library strand-specific. The completed libraries were qualified with Agilent 2100 Bioanalyzer and quantified by absolute quantification qPCR method. To sequence the libraries on the Illumina NovaSeq 6000 instrument, the barcoded libraries were mixed, denatured to single stranded DNA in NaOH, captured on Illumina flow cell, amplified in situ, and subsequently sequenced for 150 cycles for both ends on Illumina NovaSeq 6000 instrument. The completed libraries were qualified on Agilent 2100 Bioanalyzer for concentration, fragment size distribution between 400 ∼ 600 bp, and adapter dimer contamination. The amount was determined by absolute quantification qPCR method. The barcoded libraries were mixed in equal amounts and used for sequencing on the instrument. Illumina NovaSeq 6000