SRX17842106 GSM6625025_r1 SRP401827 PRJNA889017 SRS15364089 GSM6625025 GSM6625025 OTHER TRANSCRIPTOMIC other Cells were subjected to nuclear run-on for 5 minutes at 30°C with BrU labeling. The run-on RNAs were pull down by BrU beads and subjected to library preparation for deep sequencing. We followed a previous published protocol (Ingolia et al., 2009 Science; Wang et al., 2011 Nature). Briefly, the run-on RNA were first added with a polyA tracts by RNA polyA polymerase. This polyA tail enables the run-on RNA to be reverse transcribed into single strand cDNA by Reverse Transcriptase. The cDNA was circularized by CircLigase (Epicentre) and re-linearized by APE I, subsequently subjected to PCR amplification and deep sequencing. Illumina HiSeq 3000 SRX17842107 GSM6625026_r1 SRP401827 PRJNA889017 SRS15364088 GSM6625026 GSM6625026 OTHER TRANSCRIPTOMIC other Cells were subjected to nuclear run-on for 5 minutes at 30°C with BrU labeling. The run-on RNAs were pull down by BrU beads and subjected to library preparation for deep sequencing. We followed a previous published protocol (Ingolia et al., 2009 Science; Wang et al., 2011 Nature). Briefly, the run-on RNA were first added with a polyA tracts by RNA polyA polymerase. This polyA tail enables the run-on RNA to be reverse transcribed into single strand cDNA by Reverse Transcriptase. The cDNA was circularized by CircLigase (Epicentre) and re-linearized by APE I, subsequently subjected to PCR amplification and deep sequencing. Illumina HiSeq 3000 SRX17842108 GSM6625027_r1 SRP401827 PRJNA889017 SRS15364091 GSM6625027 GSM6625027 OTHER TRANSCRIPTOMIC other Cells were subjected to nuclear run-on for 5 minutes at 30°C with BrU labeling. The run-on RNAs were pull down by BrU beads and subjected to library preparation for deep sequencing. We followed a previous published protocol (Ingolia et al., 2009 Science; Wang et al., 2011 Nature). Briefly, the run-on RNA were first added with a polyA tracts by RNA polyA polymerase. This polyA tail enables the run-on RNA to be reverse transcribed into single strand cDNA by Reverse Transcriptase. The cDNA was circularized by CircLigase (Epicentre) and re-linearized by APE I, subsequently subjected to PCR amplification and deep sequencing. Illumina HiSeq 3000