SRX17899200 JI2AZA_3 SRP402646 PRJNA889596 After the QC procedures, mRNA from eukaryotic organisms is enriched using oligo (dT) beads. For prokaryotic samples, rRNA is removed first using a specialized kit before enrichment. The mRNA is then fragmented randomly in fragmentation buffer. First-strand cDNA was synthesised using random hexamers and reverse transcriptase. Then the second strand was generated using a custom second-strand synthesis buffer (Illumina) with dNTPs, RNase H and Escherichia coli polymerase I by nick-translation. The final cDNA library is ready after purification, terminal repair, A-tailing, adapter ligation, size selection and PCR enrichment. (Novoene protocol) SRS15415938 SAMN31251320 JI2AZA_3 RNA-Seq TRANSCRIPTOMIC PolyA Illumina HiSeq 4000 SRX17899201 JI2AZA_1 SRP402646 PRJNA889596 After the QC procedures, mRNA from eukaryotic organisms is enriched using oligo (dT) beads. For prokaryotic samples, rRNA is removed first using a specialized kit before enrichment. The mRNA is then fragmented randomly in fragmentation buffer. First-strand cDNA was synthesised using random hexamers and reverse transcriptase. Then the second strand was generated using a custom second-strand synthesis buffer (Illumina) with dNTPs, RNase H and Escherichia coli polymerase I by nick-translation. The final cDNA library is ready after purification, terminal repair, A-tailing, adapter ligation, size selection and PCR enrichment. (Novoene protocol) SRS15415939 SAMN31251321 JI2AZA_1 RNA-Seq TRANSCRIPTOMIC PolyA Illumina HiSeq 4000 SRX17899202 JI2AZA_2 SRP402646 PRJNA889596 After the QC procedures, mRNA from eukaryotic organisms is enriched using oligo (dT) beads. For prokaryotic samples, rRNA is removed first using a specialized kit before enrichment. The mRNA is then fragmented randomly in fragmentation buffer. First-strand cDNA was synthesised using random hexamers and reverse transcriptase. Then the second strand was generated using a custom second-strand synthesis buffer (Illumina) with dNTPs, RNase H and Escherichia coli polymerase I by nick-translation. The final cDNA library is ready after purification, terminal repair, A-tailing, adapter ligation, size selection and PCR enrichment. (Novoene protocol) SRS15415940 SAMN31251322 JI2AZA_2 RNA-Seq TRANSCRIPTOMIC PolyA Illumina HiSeq 4000 SRX17899203 JR2AZA_1 SRP402646 PRJNA889596 After the QC procedures, mRNA from eukaryotic organisms is enriched using oligo (dT) beads. For prokaryotic samples, rRNA is removed first using a specialized kit before enrichment. The mRNA is then fragmented randomly in fragmentation buffer. First-strand cDNA was synthesised using random hexamers and reverse transcriptase. Then the second strand was generated using a custom second-strand synthesis buffer (Illumina) with dNTPs, RNase H and Escherichia coli polymerase I by nick-translation. The final cDNA library is ready after purification, terminal repair, A-tailing, adapter ligation, size selection and PCR enrichment. (Novoene protocol) SRS15415941 SAMN31251341 JR2AZA_1 RNA-Seq TRANSCRIPTOMIC PolyA Illumina HiSeq 4000 SRX17899204 JR2AZA_3 SRP402646 PRJNA889596 After the QC procedures, mRNA from eukaryotic organisms is enriched using oligo (dT) beads. For prokaryotic samples, rRNA is removed first using a specialized kit before enrichment. The mRNA is then fragmented randomly in fragmentation buffer. First-strand cDNA was synthesised using random hexamers and reverse transcriptase. Then the second strand was generated using a custom second-strand synthesis buffer (Illumina) with dNTPs, RNase H and Escherichia coli polymerase I by nick-translation. The final cDNA library is ready after purification, terminal repair, A-tailing, adapter ligation, size selection and PCR enrichment. (Novoene protocol) SRS15415942 SAMN31251342 JR2AZA_3 RNA-Seq TRANSCRIPTOMIC PolyA Illumina HiSeq 4000 SRX17899205 JR2AZA_2 SRP402646 PRJNA889596 After the QC procedures, mRNA from eukaryotic organisms is enriched using oligo (dT) beads. For prokaryotic samples, rRNA is removed first using a specialized kit before enrichment. The mRNA is then fragmented randomly in fragmentation buffer. First-strand cDNA was synthesised using random hexamers and reverse transcriptase. Then the second strand was generated using a custom second-strand synthesis buffer (Illumina) with dNTPs, RNase H and Escherichia coli polymerase I by nick-translation. The final cDNA library is ready after purification, terminal repair, A-tailing, adapter ligation, size selection and PCR enrichment. (Novoene protocol) SRS15415943 SAMN31251343 JR2AZA_2 RNA-Seq TRANSCRIPTOMIC PolyA Illumina HiSeq 4000