SRX17909980 HTS data of cells untreated with base editor corresponding to CDA, TDG_g1, TDG_g2, IDH2 and FANCF sites SRP402916 bp0 HEK293T cells untreated with editing agents SRS15426235 Untreated_CDA-TDG_g1-TDG_g2-IDH2-FANCF HTS data of cells untreated with base editor corresponding to CDA, TDG_g1, TDG_g2, IDH2 and FANCF sites AMPLICON GENOMIC PCR Illumina MiSeq SRX17909981 HTS data of cells untreated with base editor corresponding to d2GFP site SRP402916 bp0 HEK293T cells untreated with editing agents SRS15426236 Untreated_d2GFP HTS data of cells untreated with base editor corresponding to d2GFP site AMPLICON GENOMIC PCR Illumina MiSeq SRX17909982 HTS data of cells treated with AIDC1*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep1 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426237 AIDC1*-BE3-d2GFP-Rep1 HTS data of cells treated with AIDC1*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep1 AMPLICON GENOMIC PCR Illumina MiSeq SRX17909983 HTS data of cells treated with AIDC1*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep2 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426238 AIDC1*-BE3-d2GFP-Rep2 HTS data of cells treated with AIDC1*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep2 AMPLICON GENOMIC PCR Illumina MiSeq SRX17909984 HTS data of cells treated with AIDC1*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep3 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426239 AIDC1*-BE3-d2GFP-Rep3 HTS data of cells treated with AIDC1*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep3 AMPLICON GENOMIC PCR Illumina MiSeq SRX17909985 HTS data of cells treated with AIDC2*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep1 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426240 AIDC2*-BE3-d2GFP-Rep1 HTS data of cells treated with AIDC2*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep1 AMPLICON GENOMIC PCR Illumina MiSeq SRX17909986 HTS data of cells treated with AIDC2*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep2 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426242 AIDC2*-BE3-d2GFP-Rep2 HTS data of cells treated with AIDC2*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep2 AMPLICON GENOMIC PCR Illumina MiSeq SRX17909987 HTS data of cells treated with AIDC2*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep3 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426241 AIDC2*-BE3-d2GFP-Rep3 HTS data of cells treated with AIDC2*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep3 AMPLICON GENOMIC PCR Illumina MiSeq SRX17909988 HTS data of cells treated with AIDC12*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep1 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426244 AIDC12*-BE3-d2GFP-Rep1 HTS data of cells treated with AIDC12*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep1 AMPLICON GENOMIC PCR Illumina MiSeq SRX17909989 HTS data of cells treated with AIDC12*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep2 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426243 AIDC12*-BE3-d2GFP-Rep2 HTS data of cells treated with AIDC12*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep2 AMPLICON GENOMIC PCR Illumina MiSeq SRX17909990 HTS data of cells treated with AIDC12*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep3 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426245 AIDC12*-BE3-d2GFP-Rep3 HTS data of cells treated with AIDC12*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep3 AMPLICON GENOMIC PCR Illumina MiSeq SRX17909991 HTS data of cells treated with AID-WT-BE3 base editor and TDG sgRNA, Figure SI3. Also, HTS data for off-target analysis of cells treated with AIDC12*dCas9-BE4max base editor and EMX1 sgRNA, Figure 3-Rep1 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426246 AID-WT-BE3-TDG-Rep1_AIDC12*-dCas9-BE4max-EMX1-OFF1-Rep1_EMX1-OFF2_Rep1 HTS data of cells treated with AID-WT-BE3 base editor and TDG sgRNA, Figure S2. Also, HTS data for off-target analysis of cells treated with AIDC12*dCas9-BE4max base editor and EMX1 sgRNA, Figure S9-Rep1 AMPLICON GENOMIC PCR Illumina MiSeq SRX17909992 HTS data of cells untreated with base editor corresponding to EMX1_OFF1, EMX1_OFF2, FANCF_OFF1 and FANCF_OFF2 sites SRP402916 bp0 HEK293T cells untreated with editing agents SRS15426247 Untreated_EMX1-OFF1_EMX1-OFF2_FANCF-OFF1_FANCF-OFF2 HTS data of cells untreated with base editor corresponding to EMX1_OFF1, EMX1_OFF2, FANCF_OFF1 and FANCF_OFF2 sites AMPLICON GENOMIC PCR Illumina MiSeq SRX17909993 HTS data of cells treated with AID-WT-BE3 base editor and TDG sgRNA, Figure SI3. Also, HTS data for off-target analysis of cells treated with AIDC12*dCas9-BE4max base editor and EMX1 sgRNA, Figure 3-Rep2 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426248 AID-WT-BE3-TDG-Rep2_AIDC12*-dCas9-BE4max-EMX1-OFF1-Rep2_EMX1-OFF2_Rep2 HTS data of cells treated with AID-WT-BE3 base editor and TDG sgRNA, Figure S2. Also, HTS data for off-target analysis of cells treated with AIDC12*dCas9-BE4max base editor and EMX1 sgRNA, Figure S9-Rep2 AMPLICON GENOMIC PCR Illumina MiSeq SRX17909994 HTS data of cells treated with AID-WT-BE3 base editor and TDG sgRNA, Figure SI3. Also, HTS data for off-target analysis of cells treated with AIDC12*dCas9-BE4max base editor and EMX1 sgRNA, Figure 3-Rep3 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426249 AID-WT-BE3-TDG-Rep3_AIDC12*-dCas9-BE4max-EMX1-OFF1-Rep3_EMX1-OFF2_Rep3 HTS data of cells treated with AID-WT-BE3 base editor and TDG sgRNA, Figure S2. Also, HTS data for off-target analysis of cells treated with AIDC12*dCas9-BE4max base editor and EMX1 sgRNA, Figure S9-Rep3 AMPLICON GENOMIC PCR Illumina MiSeq SRX17909995 HTS data of cells treated with AID*-BE3 base editor and TDG sgRNA, Figure SI3. Also, HTS data for off-target analysis of cells treated with AIDC12*nCas9-BE4max base editor and EMX1 sgRNA, Figure 3-Rep1 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426250 AID*-BE3-TDG-Rep1_AIDC12*-nCas9-BE4max-EMX1-OFF1-Rep1_EMX1-OFF2_Rep1 HTS data of cells treated with AID*-BE3 base editor and TDG sgRNA, Figure S2. Also, HTS data for off-target analysis of cells treated with AIDC12*nCas9-BE4max base editor and EMX1 sgRNA, Figure S9-Rep1 AMPLICON GENOMIC PCR Illumina MiSeq SRX17909996 HTS data of cells treated with AID*-BE3 base editor and TDG sgRNA, Figure SI3. Also, HTS data for off-target analysis of cells treated with AIDC12*nCas9-BE4max base editor and EMX1 sgRNA, Figure 3-Rep2 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426251 AID*-BE3-TDG-Rep2_AIDC12*-nCas9-BE4max-EMX1-OFF1-Rep2_EMX1-OFF2_Rep2 HTS data of cells treated with AID*-BE3 base editor and TDG sgRNA, Figure S2. Also, HTS data for off-target analysis of cells treated with AIDC12*nCas9-BE4max base editor and EMX1 sgRNA, Figure S9-Rep2 AMPLICON GENOMIC PCR Illumina MiSeq SRX17909997 HTS data of cells treated with AID*-BE3 base editor and TDG sgRNA, Figure SI3. Also, HTS data for off-target analysis of cells treated with AIDC12*nCas9-BE4max base editor and EMX1 sgRNA, Figure 3-Rep3 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426252 AID*-BE3-TDG-Rep3_AIDC12*-nCas9-BE4max-EMX1-OFF1-Rep3_EMX1-OFF2_Rep3 HTS data of cells treated with AID*-BE3 base editor and TDG sgRNA, Figure S2. Also, HTS data for off-target analysis of cells treated with AIDC12*nCas9-BE4max base editor and EMX1 sgRNA, Figure S9-Rep3 AMPLICON GENOMIC PCR Illumina MiSeq SRX17909998 HTS data of cells treated with AIDC1*-BE3 base editor and TDG sgRNA, Figure SI3. Also, HTS data for off-target analysis of cells treated with AIDC12*nCas9-BE4max base editor and FANCF sgRNA, Figure 3-Rep1 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426253 AIDC1*-BE3-TDG-Rep1_AIDC12*-nCas9-BE4max-FANCF-OFF1-Rep1_FANCF-OFF2-Rep1 HTS data of cells treated with AIDC1*-BE3 base editor and TDG sgRNA, Figure S2. Also, HTS data for off-target analysis of cells treated with AIDC12*nCas9-BE4max base editor and FANCF sgRNA, Figure S9-Rep1 AMPLICON GENOMIC PCR Illumina MiSeq SRX17909999 HTS data of cells treated with AIDC1*-BE3 base editor and TDG sgRNA, Figure SI3. Also, HTS data for off-target analysis of cells treated with AIDC12*nCas9-BE4max base editor and FANCF sgRNA, Figure 3-Rep2 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426254 AIDC1*-BE3-TDG-Rep2_AIDC12*-nCas9-BE4max-FANCF-OFF1-Rep2_FANCF-OFF2-Rep2 HTS data of cells treated with AIDC1*-BE3 base editor and TDG sgRNA, Figure S2. Also, HTS data for off-target analysis of cells treated with AIDC12*nCas9-BE4max base editor and FANCF sgRNA, Figure S9-Rep2 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910000 HTS data of cells treated with AIDC1*-BE3 base editor and TDG sgRNA, Figure SI3. Also, HTS data for off-target analysis of cells treated with AIDC12*nCas9-BE4max base editor and FANCF sgRNA, Figure 3-Rep3 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426255 AIDC1*-BE3-TDG-Rep3_AIDC12*-nCas9-BE4max-FANCF-OFF1-Rep3_FANCF-OFF2-Rep3 HTS data of cells treated with AIDC1*-BE3 base editor and TDG sgRNA, Figure S2. Also, HTS data for off-target analysis of cells treated with AIDC12*nCas9-BE4max base editor and FANCF sgRNA, Figure S9-Rep3 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910001 HTS data of cells treated with AIDC2*-BE3 base editor and TDG sgRNA, Figure SI3. Also, HTS data for off-target analysis of cells treated with AIDC12*n'Cas9-BE4max base editor and EMX1 sgRNA, Figure 3-Rep1 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426256 AIDC2*-BE3-TDG-Rep1_AIDC12*-n'Cas9-BE4max-EMX1-OFF1-Rep1_EMX1-OFF2_Rep1 HTS data of cells treated with AIDC2*-BE3 base editor and TDG sgRNA, Figure S2. Also, HTS data for off-target analysis of cells treated with AIDC12*n'Cas9-BE4max base editor and EMX1 sgRNA, Figure S9-Rep1 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910002 HTS data of cells treated with AIDC2*-BE3 base editor and TDG sgRNA, Figure SI3. Also, HTS data for off-target analysis of cells treated with AIDC12*n'Cas9-BE4max base editor and EMX1 sgRNA, Figure 3-Rep2 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426257 AIDC2*-BE3-TDG-Rep2_AIDC12*-n'Cas9-BE4max-EMX1-OFF1-Rep2_EMX1-OFF2_Rep2 HTS data of cells treated with AIDC2*-BE3 base editor and TDG sgRNA, Figure S2. Also, HTS data for off-target analysis of cells treated with AIDC12*n'Cas9-BE4max base editor and EMX1 sgRNA, Figure S9-Rep2 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910003 HTS data of cells untreated with base editor corresponding to EMX1_ON (on target) and dSaCas9 sites SRP402916 bp0 HEK293T cells untreated with editing agents SRS15426258 Untreated_EMX1-ON_dSaCas9 HTS data of cells untreated with base editor corresponding to EMX1_ON (on target) and dSaCas9 sites AMPLICON GENOMIC PCR Illumina MiSeq SRX17910004 HTS data of cells treated with AIDC2*-BE3 base editor and TDG sgRNA, Figure SI3. Also, HTS data for off-target analysis of cells treated with AIDC12*n'Cas9-BE4max base editor and EMX1 sgRNA, Figure 3-Rep3 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426259 AIDC2*-BE3-TDG-Rep3_AIDC12*-n'Cas9-BE4max-EMX1-OFF1-Rep3_EMX1-OFF2_Rep3 HTS data of cells treated with AIDC2*-BE3 base editor and TDG sgRNA, Figure S2. Also, HTS data for off-target analysis of cells treated with AIDC12*n'Cas9-BE4max base editor and EMX1 sgRNA, Figure S9-Rep3 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910005 HTS data of cells treated with AIDC2*-BE3 base editor and TDG sgRNA, Figure SI3. Also, HTS data for off-target analysis of cells treated with AIDC12*n'Cas9-BE4max base editor and FANCF sgRNA, Figure 3-Rep1 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426260 AIDC12*-BE3-TDG-Rep1_AIDC12*-n'Cas9-BE4max-FANCF-OFF1-Rep1_FANCF-OFF2-Rep1 HTS data of cells treated with AIDC2*-BE3 base editor and TDG sgRNA, Figure S2. Also, HTS data for off-target analysis of cells treated with AIDC12*n'Cas9-BE4max base editor and FANCF sgRNA, Figure S9-Rep1 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910006 HTS data of cells treated with AIDC2*-BE3 base editor and TDG sgRNA, Figure SI3. Also, HTS data for off-target analysis of cells treated with AIDC12*n'Cas9-BE4max base editor and FANCF sgRNA, Figure 3-Rep2 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426261 AIDC12*-BE3-TDG-Rep2_AIDC12*-n'Cas9-BE4max-FANCF-OFF1-Rep2_FANCF-OFF2-Rep2 HTS data of cells treated with AIDC2*-BE3 base editor and TDG sgRNA, Figure S2. Also, HTS data for off-target analysis of cells treated with AIDC12*n'Cas9-BE4max base editor and FANCF sgRNA, Figure S9-Rep2 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910007 HTS data of cells treated with AIDC2*-BE3 base editor and TDG sgRNA, Figure SI3. Also, HTS data for off-target analysis of cells treated with AIDC12*n'Cas9-BE4max base editor and FANCF sgRNA, Figure 3-Rep3 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426262 AIDC12*-BE3-TDG-Rep3_AIDC12*-n'Cas9-BE4max-FANCF-OFF1-Rep3_FANCF-OFF2-Rep3 HTS data of cells treated with AIDC2*-BE3 base editor and TDG sgRNA, Figure S2. Also, HTS data for off-target analysis of cells treated with AIDC12*n'Cas9-BE4max base editor and FANCF sgRNA, Figure S9-Rep3 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910008 HTS data for off-target analysis of cells treated with AIDC12*dCas9-BE4max base editor and FANCF sgRNA, Figure 3-Rep1 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426264 AIDC12*-dCas9-BE4max-FANCF-OFF1-Rep1_FANCF-OFF2-Rep1 HTS data for off-target analysis of cells treated with AIDC12*dCas9-BE4max base editor and FANCF sgRNA, Figure S9-Rep1 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910009 HTS data for off-target analysis of cells treated with AIDC12*dCas9-BE4max base editor and FANCF sgRNA, Figure 3-Rep2 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426263 AIDC12*-dCas9-BE4max-FANCF-OFF1-Rep2_FANCF-OFF2-Rep2 HTS data for off-target analysis of cells treated with AIDC12*dCas9-BE4max base editor and FANCF sgRNA, Figure S9-Rep2 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910010 HTS data of cells treated with AID-WT-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4-Rep1 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426265 AID-WT-BE3_EMX1-ON_EMX1-OFF1_EMX1-OFF2_dSaCas9_Rep1 HTS data of cells treated with AID-WT-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4-Rep1 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910011 HTS data of cells treated with AID-WT-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4-Rep2 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426266 AID-WT-BE3_EMX1-ON_EMX1-OFF1_EMX1-OFF2_dSaCas9_Rep2 HTS data of cells treated with AID-WT-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4-Rep2 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910012 HTS data of cells treated with AID-WT-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4-Rep3 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426268 AID-WT-BE3_EMX1-ON_EMX1-OFF1_EMX1-OFF2_dSaCas9_Rep3 HTS data of cells treated with AID-WT-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4-Rep3 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910013 HTS data of cells treated with AID*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4-Rep1 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426267 AID*-BE3_EMX1-ON_EMX1-OFF1_EMX1-OFF2_dSaCas9_Rep1 HTS data of cells treated with AID*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4-Rep1 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910014 HTS data of cells treated with AID-WT-BE3 base editor and d2GFP sgRNA, Figure 2-Rep1 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426269 AID-WT-BE3-d2GFP-Rep1 HTS data of cells treated with AID-WT-BE3 base editor and d2GFP sgRNA, Figure 2-Rep1 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910015 HTS data of cells treated with AID*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4-Rep2 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426270 AID*-BE3_EMX1-ON_EMX1-OFF1_EMX1-OFF2_dSaCas9_Rep2 HTS data of cells treated with AID*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4-Rep2 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910016 HTS data of cells treated with AID*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1 andEMX1_OFF2, Figure 2 and SI Figures 3 and 4-Rep3 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426271 AID*-BE3_EMX1-ON_EMX1-OFF1_EMX1-OFF2_Rep3 HTS data of cells treated with AID*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1 andEMX1_OFF2, Figure 2 and SI Figures 3 and 4-Rep3 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910017 HTS data for off-target analysis of cells treated with AID*-BE3 base editor and EMX1 sgRNA for dSaCas9 site, Figure 2 and SI Figures 3 and 4-Rep3 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426272 AID*-BE3-dSaCas9-Rep3 HTS data for off-target analysis of cells treated with AID*-BE3 base editor and EMX1 sgRNA for dSaCas9 site, Figure 2 and SI Figures 3 and 4-Rep3 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910018 HTS data of cells treated with AIDC1*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4. Also, HTS data of cells treated with AIDC12*-dCas9-BE4max base editor and d2GF SRP402916 bp0 HEK293T cells treated with editing agents SRS15426273 AIDC1*-BE3_EMX1-ON_EMX1-OFF1_EMX1-OFF2_dSaCas9_Rep1_and_AIDC12*-dCas9-BE4max-d2GFP-Rep1 HTS data of cells treated with AIDC1*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4. Also, HTS data of cells treated with AIDC12*-dCas9-BE4max base editor and d2GFP sgRNA, Figure 3. Rep1 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910019 HTS data of cells treated with AIDC1*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4-Rep2 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426274 AIDC1*-BE3_EMX1-ON_EMX1-OFF1_EMX1-OFF2_dSaCas9_Rep2 HTS data of cells treated with AIDC1*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4-Rep2 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910020 HTS data of cells treated with AIDC1*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4-Rep3 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426275 AIDC1*-BE3_EMX1-ON_EMX1-OFF1_EMX1-OFF2_dSaCas9_Rep3 HTS data of cells treated with AIDC1*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4-Rep3 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910021 HTS data of cells treated with AIDC2*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4. Also, HTS data of cells treated with AIDC12*-n'Cas9-BE4max base editor and d2G SRP402916 bp0 HEK293T cells treated with editing agents SRS15426276 AIDC2*-BE3_EMX1-ON_EMX1-OFF1_EMX1-OFF2_dSaCas9_Rep1_and_AIDC12*-n'Cas9-BE4max-d2GFP-Rep1 HTS data of cells treated with AIDC2*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4. Also, HTS data of cells treated with AIDC12*-n'Cas9-BE4max base editor and d2GFP sgRNA, Figure 3. Rep1 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910022 HTS data of cells treated with AIDC2*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4. Rep2 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426277 AIDC2*-BE3_EMX1-ON_EMX1-OFF1_EMX1-OFF2_dSaCas9_Rep2 HTS data of cells treated with AIDC2*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4. Rep2 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910023 HTS data of cells treated with AIDC2*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4. Rep3 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426278 AIDC2*-BE3_EMX1-ON_EMX1-OFF1_EMX1-OFF2_dSaCas9_Rep3 HTS data of cells treated with AIDC2*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4. Rep3 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910024 HTS data of cells treated with AIDC12*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4. Also, HTS data of cells treated with AIDC12*-BE3 base editor and d2GFP sgRNA SRP402916 bp0 HEK293T cells treated with editing agents SRS15426279 AIDC12*-BE3_EMX1-ON_EMX1-OFF1_EMX1-OFF2_dSaCas9_Rep1_and_AIDC12*-BE3-d2GFP-Rep1 HTS data of cells treated with AIDC12*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4. Also, HTS data of cells treated with AIDC12*-BE3 base editor and d2GFP sgRNA, Figure 3. Rep1 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910025 HTS data of cells treated with AID-WT-BE3 base editor and d2GFP sgRNA, Figure 2-Rep2 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426280 AID-WT-BE3-d2GFP-Rep2 HTS data of cells treated with AID-WT-BE3 base editor and d2GFP sgRNA, Figure 2-Rep2 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910026 HTS data of cells treated with AIDC12*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4. Rep2 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426281 AIDC12*-BE3_EMX1-ON_EMX1-OFF1_EMX1-OFF2_dSaCas9_Rep2 HTS data of cells treated with AIDC12*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4. Rep2 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910027 HTS data of cells treated with AIDC12*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4. Rep3 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426282 AIDC12*-BE3_EMX1-ON_EMX1-OFF1_EMX1-OFF2_dSaCas9_Rep3 HTS data of cells treated with AIDC12*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4. Rep3 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910028 HTS data of cells treated with AIDC12*-nCas9-BE4max base editor and d2GFP sgRNA, Figure 3. Rep1 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426283 AIDC12*-nCas9-BE4max-d2GFP-Rep1 HTS data of cells treated with AIDC12*-nCas9-BE4max base editor and d2GFP sgRNA, Figure 3. Rep1 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910029 HTS data of cells treated with AIDC12*-dCas9-BE4max base editor and sgRNAs CDA, TDG_g1, TDG_g2, IDH2, EMX1, and FANCF. Figure 3. Rep1 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426284 AIDC12*-dCas9-BE4max_CDA-TDG_g1-TDG_g2-IDH2-EMX1-FANCF_Rep1 HTS data of cells treated with AIDC12*-dCas9-BE4max base editor and sgRNAs CDA, TDG_g1, TDG_g2, IDH2, EMX1, and FANCF. Figure 3. Rep1 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910030 HTS data of cells treated with AIDC12*-dCas9-BE4max base editor and sgRNAs d2GFP, CDA, TDG_g1, TDG_g2, IDH2, EMX1, and FANCF. Figure 3. Rep2 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426285 AIDC12*-dCas9-BE4max_d2GFP-CDA-TDG_g1-TDG_g2-IDH2-EMX1-FANCF_Rep2 HTS data of cells treated with AIDC12*-dCas9-BE4max base editor and sgRNAs d2GFP, CDA, TDG_g1, TDG_g2, IDH2, EMX1, and FANCF. Figure 3. Rep2 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910031 HTS data of cells treated with AIDC12*-dCas9-BE4max base editor and sgRNAs d2GFP, CDA, TDG_g1, TDG_g2, IDH2, EMX1, and FANCF. Figure 3. Rep3 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426286 AIDC12*-dCas9-BE4max_d2GFP-CDA-TDG_g1-TDG_g2-IDH2-EMX1-FANCF_Rep3 HTS data of cells treated with AIDC12*-dCas9-BE4max base editor and sgRNAs d2GFP, CDA, TDG_g1, TDG_g2, IDH2, EMX1, and FANCF. Figure 3. Rep3 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910032 HTS data of cells treated with AIDC12*-n'Cas9-BE4max base editor and sgRNAs CDA, TDG_g1, TDG_g2, IDH2, EMX1, and FANCF, Figure 3. Also, HTS data of cells treated with AIDC12*BE3 base editor and sgRNA d2GFP SI Figure 5. Rep1 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426287 AIDC12*-n'Cas9-BE4max_CDA-TDG_g1-TDG_g2-IDH2-EMX1-FANCF_Rep1_and_AIDC12*-BE3-d2GFP_Rep3 HTS data of cells treated with AIDC12*-n'Cas9-BE4max base editor and sgRNAs CDA, TDG_g1, TDG_g2, IDH2, EMX1, and FANCF, Figure 3. Rep1 Also, HTS data of cells treated with AIDC12*BE3 base editor and sgRNA d2GFP Figure S7. Rep3 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910033 HTS data of cells treated with AIDC12*-n'Cas9-BE4max base editor and sgRNAs d2GFP, CDA, TDG_g1, TDG_g2, IDH2, EMX1, and FANCF, Figure 3. Rep2 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426288 AIDC12*-n'Cas9-BE4max_d2GFP-CDA-TDG_g1-TDG_g2-IDH2-EMX1-FANCF_Rep2 HTS data of cells treated with AIDC12*-n'Cas9-BE4max base editor and sgRNAs d2GFP, CDA, TDG_g1, TDG_g2, IDH2, EMX1, and FANCF, Figure 3. Rep2 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910034 HTS data of cells treated with AIDC12*-n'Cas9-BE4max base editor and sgRNAs d2GFP, CDA, TDG_g1, TDG_g2, IDH2, EMX1, and FANCF, Figure 3. Rep3 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426289 AIDC12*-n'Cas9-BE4max_d2GFP-CDA-TDG_g1-TDG_g2-IDH2-EMX1-FANCF_Rep3 HTS data of cells treated with AIDC12*-n'Cas9-BE4max base editor and sgRNAs d2GFP, CDA, TDG_g1, TDG_g2, IDH2, EMX1, and FANCF, Figure 3. Rep3 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910035 HTS data of cells treated with AIDC12*-nCas9-BE4max base editor and sgRNAs CDA, TDG_g1, TDG_g2, IDH2, EMX1, and FANCF, Figure 3. Also, HTS data of cells treated with AIDC12*BE3 base editor and sgRNA d2GFP SI Figure 5. Rep1 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426290 AIDC12*-nCas9-BE4max_CDA-TDG_g1-TDG_g2-IDH2-EMX1-FANCF_Rep1_and_AIDC12*-BE3-d2GFP_Rep2 HTS data of cells treated with AIDC12*-nCas9-BE4max base editor and sgRNAs CDA, TDG_g1, TDG_g2, IDH2, EMX1, and FANCF, Figure 3. Rep 1 Also, HTS data of cells treated with AIDC12*BE3 base editor and sgRNA d2GFP Figure S7. Rep 2 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910036 HTS data of cells treated with AID-WT-BE3 base editor and d2GFP sgRNA, Figure 2-Rep3 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426291 AID-WT-BE3-d2GFP-Rep3 HTS data of cells treated with AID-WT-BE3 base editor and d2GFP sgRNA, Figure 2-Rep3 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910037 HTS data of cells treated with AIDC12*-nCas9-BE4max base editor and sgRNAs d2GFP, CDA, TDG_g1, TDG_g2, IDH2, EMX1, and FANCF, Figure 3. Rep2 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426292 AIDC12*-nCas9-BE4max_d2GFP-CDA-TDG_g1-TDG_g2-IDH2-EMX1-FANCF_Rep2 HTS data of cells treated with AIDC12*-nCas9-BE4max base editor and sgRNAs d2GFP, CDA, TDG_g1, TDG_g2, IDH2, EMX1, and FANCF, Figure 3. Rep2 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910038 HTS data of cells treated with AIDC12*-nCas9-BE4max base editor and sgRNAs d2GFP, CDA, TDG_g1, TDG_g2, IDH2, EMX1, and FANCF, Figure 3. Rep3 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426293 AIDC12*-nCas9-BE4max_d2GFP-CDA-TDG_g1-TDG_g2-IDH2-EMX1-FANCF_Rep3 HTS data of cells treated with AIDC12*-nCas9-BE4max base editor and sgRNAs d2GFP, CDA, TDG_g1, TDG_g2, IDH2, EMX1, and FANCF, Figure 3. Rep3 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910039 HTS data of cells treated with AID*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep1 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426294 AID*-BE3-d2GFP-Rep1 HTS data of cells treated with AID*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep1 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910040 HTS data of cells treated with AID*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep2 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426295 AID*-BE3-d2GFP-Rep2 HTS data of cells treated with AID*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep2 AMPLICON GENOMIC PCR Illumina MiSeq SRX17910041 HTS data of cells treated with AID*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep3 SRP402916 bp0 HEK293T cells treated with editing agents SRS15426296 AID*-BE3-d2GFP-Rep3 HTS data of cells treated with AID*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep3 AMPLICON GENOMIC PCR Illumina MiSeq