SRR21924861 AID-BE3-d2GFP-Rep3_R1.fastq.gz HTS data of cells treated with AID*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep3 SRR21924862 AID-BE3-d2GFP-Rep2_R1.fastq.gz HTS data of cells treated with AID*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep2 SRR21924863 AIDC12-nCas9-BE4max_d2GFP-CDA-TDG_g1-TDG_g2-IDH2-EMX1-FANCF_Rep3_R1.fastq.gz HTS data of cells treated with AIDC12*-nCas9-BE4max base editor and sgRNAs d2GFP, CDA, TDG_g1, TDG_g2, IDH2, EMX1, and FANCF, Figure 3. Rep3 SRR21924864 AIDC12-nCas9-BE4max_d2GFP-CDA-TDG_g1-TDG_g2-IDH2-EMX1-FANCF_Rep2_R1.fastq.gz HTS data of cells treated with AIDC12*-nCas9-BE4max base editor and sgRNAs d2GFP, CDA, TDG_g1, TDG_g2, IDH2, EMX1, and FANCF, Figure 3. Rep2 SRR21924865 AID-WT-BE3-d2GFP-Rep3_R1.fastq.gz HTS data of cells treated with AID-WT-BE3 base editor and d2GFP sgRNA, Figure 2-Rep3 SRR21924866 AID-BE3-d2GFP-Rep1_R1.fastq.gz HTS data of cells treated with AID*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep1 SRR21924867 AIDC12-nCas9-BE4max_CDA-TDG_g1-TDG_g2-IDH2-EMX1-FANCF_Rep1_and_AIDC12-BE3-d2GFP_Rep2_R1.fastq.gz HTS data of cells treated with AIDC12*-nCas9-BE4max base editor and sgRNAs CDA, TDG_g1, TDG_g2, IDH2, EMX1, and FANCF, Figure 3. Also, HTS data of cells treated with AIDC12*BE3 base editor and sgRNA d2GFP SI Figure 5. Rep1 SRR21924868 AIDC12-nprCas9-BE4max_d2GFP-CDA-TDG_g1-TDG_g2-IDH2-EMX1-FANCF_Rep3_R1.fastq.gz HTS data of cells treated with AIDC12*-n'Cas9-BE4max base editor and sgRNAs d2GFP, CDA, TDG_g1, TDG_g2, IDH2, EMX1, and FANCF, Figure 3. Rep3 SRR21924869 AIDC12-nprCas9-BE4max_d2GFP-CDA-TDG_g1-TDG_g2-IDH2-EMX1-FANCF_Rep2_R1.fastq.gz HTS data of cells treated with AIDC12*-n'Cas9-BE4max base editor and sgRNAs d2GFP, CDA, TDG_g1, TDG_g2, IDH2, EMX1, and FANCF, Figure 3. Rep2 SRR21924870 AIDC12-nprCas9-BE4max_CDA-TDG_g1-TDG_g2-IDH2-EMX1-FANCF_Rep1_and_AIDC12-BE3-d2GFP_Rep3_R1.fastq.gz HTS data of cells treated with AIDC12*-n'Cas9-BE4max base editor and sgRNAs CDA, TDG_g1, TDG_g2, IDH2, EMX1, and FANCF, Figure 3. Also, HTS data of cells treated with AIDC12*BE3 base editor and sgRNA d2GFP SI Figure 5. Rep1 SRR21924871 AIDC12-dCas9-BE4max_d2GFP-CDA-TDG_g1-TDG_g2-IDH2-EMX1-FANCF_Rep3_R1.fastq.gz HTS data of cells treated with AIDC12*-dCas9-BE4max base editor and sgRNAs d2GFP, CDA, TDG_g1, TDG_g2, IDH2, EMX1, and FANCF. Figure 3. Rep3 SRR21924872 AIDC12-dCas9-BE4max_d2GFP-CDA-TDG_g1-TDG_g2-IDH2-EMX1-FANCF_Rep2_R1.fastq.gz HTS data of cells treated with AIDC12*-dCas9-BE4max base editor and sgRNAs d2GFP, CDA, TDG_g1, TDG_g2, IDH2, EMX1, and FANCF. Figure 3. Rep2 SRR21924873 AIDC12-dCas9-BE4max_CDA-TDG_g1-TDG_g2-IDH2-EMX1-FANCF_Rep1_R1.fastq.gz HTS data of cells treated with AIDC12*-dCas9-BE4max base editor and sgRNAs CDA, TDG_g1, TDG_g2, IDH2, EMX1, and FANCF. Figure 3. Rep1 SRR21924874 AIDC12-nCas9-BE4max-d2GFP-Rep1_R1.fastq.gz HTS data of cells treated with AIDC12*-nCas9-BE4max base editor and d2GFP sgRNA, Figure 3. Rep1 SRR21924875 AIDC12-BE3_EMX1-ON_EMX1-OFF1_EMX1-OFF2_dSaCas9_Rep3_R1.fastq.gz HTS data of cells treated with AIDC12*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4. Rep3 SRR21924876 AIDC12-BE3_EMX1-ON_EMX1-OFF1_EMX1-OFF2_dSaCas9_Rep2_R1.fastq.gz HTS data of cells treated with AIDC12*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4. Rep2 SRR21924877 AID-WT-BE3-d2GFP-Rep2_R1.fastq.gz HTS data of cells treated with AID-WT-BE3 base editor and d2GFP sgRNA, Figure 2-Rep2 SRR21924878 AIDC12-BE3_EMX1-ON_EMX1-OFF1_EMX1-OFF2_dSaCas9_Rep1_and_AIDC12-BE3-d2GFP-Rep1_R1.fastq.gz HTS data of cells treated with AIDC12*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4. Also, HTS data of cells treated with AIDC12*-BE3 base editor and d2GFP sgRNA, Figure 3. Rep1 SRR21924879 AIDC2-BE3_EMX1-ON_EMX1-OFF1_EMX1-OFF2_dSaCas9_Rep3_R1.fastq.gz HTS data of cells treated with AIDC2*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4. Rep3 SRR21924880 AIDC2-BE3_EMX1-ON_EMX1-OFF1_EMX1-OFF2_dSaCas9_Rep2_R1.fastq.gz HTS data of cells treated with AIDC2*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4. Rep2 SRR21924881 AIDC2-BE3_EMX1-ON_EMX1-OFF1_EMX1-OFF2_dSaCas9_Rep1_and_AIDC12-nprCas9-BE4max-d2GFP-Rep1_R1.fastq.gz HTS data of cells treated with AIDC2*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4. Also, HTS data of cells treated with AIDC12*-n'Cas9-BE4max base editor and d2GFP sgRNA, Figure 3. Rep1 SRR21924882 AIDC1-BE3_EMX1-ON_EMX1-OFF1_EMX1-OFF2_dSaCas9_Rep3_R1.fastq.gz HTS data of cells treated with AIDC1*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4-Rep3 SRR21924883 AIDC1-BE3_EMX1-ON_EMX1-OFF1_EMX1-OFF2_dSaCas9_Rep2_R1.fastq.gz HTS data of cells treated with AIDC1*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4-Rep2 SRR21924884 AIDC1-BE3_EMX1-ON_EMX1-OFF1_EMX1-OFF2_dSaCas9_Rep1_and_AIDC12-dCas9-BE4max-d2GFP-Rep1_R1.fastq.gz HTS data of cells treated with AIDC1*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4. Also, HTS data of cells treated with AIDC12*-dCas9-BE4max base editor and d2GFP sgRNA, Figure 3. Rep1 SRR21924885 AID-BE3-dSaCas9-Rep3_R1.fastq.gz HTS data for off-target analysis of cells treated with AID*-BE3 base editor and EMX1 sgRNA for dSaCas9 site, Figure 2 and SI Figures 3 and 4-Rep3 SRR21924886 AID-BE3_EMX1-ON_EMX1-OFF1_EMX1-OFF2_Rep3_R1.fastq.gz HTS data of cells treated with AID*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1 andEMX1_OFF2, Figure 2 and SI Figures 3 and 4-Rep3 SRR21924887 AID-BE3_EMX1-ON_EMX1-OFF1_EMX1-OFF2_dSaCas9_Rep2_R1.fastq.gz HTS data of cells treated with AID*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4-Rep2 SRR21924888 AID-WT-BE3-d2GFP-Rep1_R1.fastq.gz HTS data of cells treated with AID-WT-BE3 base editor and d2GFP sgRNA, Figure 2-Rep1 SRR21924889 AID-BE3_EMX1-ON_EMX1-OFF1_EMX1-OFF2_dSaCas9_Rep1_R1.fastq.gz HTS data of cells treated with AID*-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4-Rep1 SRR21924890 AID-WT-BE3_EMX1-ON_EMX1-OFF1_EMX1-OFF2_dSaCas9_Rep3_R1.fastq.gz HTS data of cells treated with AID-WT-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4-Rep3 SRR21924891 AID-WT-BE3_EMX1-ON_EMX1-OFF1_EMX1-OFF2_dSaCas9_Rep2_R1.fastq.gz HTS data of cells treated with AID-WT-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4-Rep2 SRR21924892 AID-WT-BE3_EMX1-ON_EMX1-OFF1_EMX1-OFF2_dSaCas9_Rep1_R1.fastq.gz HTS data of cells treated with AID-WT-BE3 base editor and EMX1 sgRNA. Also, it's off-target analysis for EMX1_OFF1, EMX1_OFF2 and dSaCas9 site, Figure 2 and SI Figures 3 and 4-Rep1 SRR21924893 AIDC12-dCas9-BE4max-FANCF-OFF1-Rep2_FANCF-OFF2-Rep2_R1.fastq.gz HTS data for off-target analysis of cells treated with AIDC12*dCas9-BE4max base editor and FANCF sgRNA, Figure 3-Rep2 SRR21924894 AIDC12-dCas9-BE4max-FANCF-OFF1-Rep1_FANCF-OFF2-Rep1_R1.fastq.gz HTS data for off-target analysis of cells treated with AIDC12*dCas9-BE4max base editor and FANCF sgRNA, Figure 3-Rep1 SRR21924895 AIDC12-BE3-TDG-Rep3_AIDC12-nprCas9-BE4max-FANCF-OFF1-Rep3_FANCF-OFF2-Rep3_R1.fastq.gz HTS data of cells treated with AIDC2*-BE3 base editor and TDG sgRNA, Figure SI3. Also, HTS data for off-target analysis of cells treated with AIDC12*n'Cas9-BE4max base editor and FANCF sgRNA, Figure 3-Rep3 SRR21924896 AIDC12-BE3-TDG-Rep2_AIDC12-nprCas9-BE4max-FANCF-OFF1-Rep2_FANCF-OFF2-Rep2_R1.fastq.gz HTS data of cells treated with AIDC2*-BE3 base editor and TDG sgRNA, Figure SI3. Also, HTS data for off-target analysis of cells treated with AIDC12*n'Cas9-BE4max base editor and FANCF sgRNA, Figure 3-Rep2 SRR21924897 AIDC12-BE3-TDG-Rep1_AIDC12-nprCas9-BE4max-FANCF-OFF1-Rep1_FANCF-OFF2-Rep1_R1.fastq.gz HTS data of cells treated with AIDC2*-BE3 base editor and TDG sgRNA, Figure SI3. Also, HTS data for off-target analysis of cells treated with AIDC12*n'Cas9-BE4max base editor and FANCF sgRNA, Figure 3-Rep1 SRR21924898 AIDC2-BE3-TDG-Rep3_AIDC12-nprCas9-BE4max-EMX1-OFF1-Rep3_EMX1-OFF2_Rep3_R1.fastq.gz HTS data of cells treated with AIDC2*-BE3 base editor and TDG sgRNA, Figure SI3. Also, HTS data for off-target analysis of cells treated with AIDC12*n'Cas9-BE4max base editor and EMX1 sgRNA, Figure 3-Rep3 SRR21924899 Untreated_EMX1-ON_dSaCas9_R1.fastq.gz HTS data of cells untreated with base editor corresponding to EMX1_ON (on target) and dSaCas9 sites SRR21924900 AIDC2-BE3-TDG-Rep2_AIDC12-nprCas9-BE4max-EMX1-OFF1-Rep2_EMX1-OFF2_Rep2_R1.fastq.gz HTS data of cells treated with AIDC2*-BE3 base editor and TDG sgRNA, Figure SI3. Also, HTS data for off-target analysis of cells treated with AIDC12*n'Cas9-BE4max base editor and EMX1 sgRNA, Figure 3-Rep2 SRR21924901 AIDC2-BE3-TDG-Rep1_AIDC12-nprCas9-BE4max-EMX1-OFF1-Rep1_EMX1-OFF2_Rep1_R1.fastq.gz HTS data of cells treated with AIDC2*-BE3 base editor and TDG sgRNA, Figure SI3. Also, HTS data for off-target analysis of cells treated with AIDC12*n'Cas9-BE4max base editor and EMX1 sgRNA, Figure 3-Rep1 SRR21924902 AIDC1-BE3-TDG-Rep3_AIDC12-nCas9-BE4max-FANCF-OFF1-Rep3_FANCF-OFF2-Rep3_R1.fastq.gz HTS data of cells treated with AIDC1*-BE3 base editor and TDG sgRNA, Figure SI3. Also, HTS data for off-target analysis of cells treated with AIDC12*nCas9-BE4max base editor and FANCF sgRNA, Figure 3-Rep3 SRR21924903 AIDC1-BE3-TDG-Rep2_AIDC12-nCas9-BE4max-FANCF-OFF1-Rep2_FANCF-OFF2-Rep2_R1.fastq.gz HTS data of cells treated with AIDC1*-BE3 base editor and TDG sgRNA, Figure SI3. Also, HTS data for off-target analysis of cells treated with AIDC12*nCas9-BE4max base editor and FANCF sgRNA, Figure 3-Rep2 SRR21924904 AIDC1-BE3-TDG-Rep1_AIDC12-nCas9-BE4max-FANCF-OFF1-Rep1_FANCF-OFF2-Rep1_R1.fastq.gz HTS data of cells treated with AIDC1*-BE3 base editor and TDG sgRNA, Figure SI3. Also, HTS data for off-target analysis of cells treated with AIDC12*nCas9-BE4max base editor and FANCF sgRNA, Figure 3-Rep1 SRR21924905 AID-BE3-TDG-Rep3_AIDC12-nCas9-BE4max-EMX1-OFF1-Rep3_EMX1-OFF2_Rep3_R1.fastq.gz HTS data of cells treated with AID*-BE3 base editor and TDG sgRNA, Figure SI3. Also, HTS data for off-target analysis of cells treated with AIDC12*nCas9-BE4max base editor and EMX1 sgRNA, Figure 3-Rep3 SRR21924906 AID-BE3-TDG-Rep2_AIDC12-nCas9-BE4max-EMX1-OFF1-Rep2_EMX1-OFF2_Rep2_R1.fastq.gz HTS data of cells treated with AID*-BE3 base editor and TDG sgRNA, Figure SI3. Also, HTS data for off-target analysis of cells treated with AIDC12*nCas9-BE4max base editor and EMX1 sgRNA, Figure 3-Rep2 SRR21924907 AID-BE3-TDG-Rep1_AIDC12-nCas9-BE4max-EMX1-OFF1-Rep1_EMX1-OFF2_Rep1_R1.fastq.gz HTS data of cells treated with AID*-BE3 base editor and TDG sgRNA, Figure SI3. Also, HTS data for off-target analysis of cells treated with AIDC12*nCas9-BE4max base editor and EMX1 sgRNA, Figure 3-Rep1 SRR21924908 AID-WT-BE3-TDG-Rep3_AIDC12-dCas9-BE4max-EMX1-OFF1-Rep3_EMX1-OFF2_Rep3_R1.fastq.gz HTS data of cells treated with AID-WT-BE3 base editor and TDG sgRNA, Figure SI3. Also, HTS data for off-target analysis of cells treated with AIDC12*dCas9-BE4max base editor and EMX1 sgRNA, Figure 3-Rep3 SRR21924909 AID-WT-BE3-TDG-Rep2_AIDC12-dCas9-BE4max-EMX1-OFF1-Rep2_EMX1-OFF2_Rep2_R1.fastq.gz HTS data of cells treated with AID-WT-BE3 base editor and TDG sgRNA, Figure SI3. Also, HTS data for off-target analysis of cells treated with AIDC12*dCas9-BE4max base editor and EMX1 sgRNA, Figure 3-Rep2 SRR21924910 Untreated_EMX1-OFF1_EMX1-OFF2_FANCF-OFF1_FANCF-OFF2_R1.fastq.gz HTS data of cells untreated with base editor corresponding to EMX1_OFF1, EMX1_OFF2, FANCF_OFF1 and FANCF_OFF2 sites SRR21924911 AID-WT-BE3-TDG-Rep1_AIDC12-dCas9-BE4max-EMX1-OFF1-Rep1_EMX1-OFF2_Rep1_R1.fastq.gz HTS data of cells treated with AID-WT-BE3 base editor and TDG sgRNA, Figure SI3. Also, HTS data for off-target analysis of cells treated with AIDC12*dCas9-BE4max base editor and EMX1 sgRNA, Figure 3-Rep1 SRR21924912 AIDC12-BE3-d2GFP-Rep3_R1.fastq.gz HTS data of cells treated with AIDC12*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep3 SRR21924913 AIDC12-BE3-d2GFP-Rep2_R1.fastq.gz HTS data of cells treated with AIDC12*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep2 SRR21924914 AIDC12-BE3-d2GFP-Rep1_R1.fastq.gz HTS data of cells treated with AIDC12*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep1 SRR21924915 AIDC2-BE3-d2GFP-Rep3_R1.fastq.gz HTS data of cells treated with AIDC2*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep3 SRR21924916 AIDC2-BE3-d2GFP-Rep2_R1.fastq.gz HTS data of cells treated with AIDC2*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep2 SRR21924917 AIDC2-BE3-d2GFP-Rep1_R1.fastq.gz HTS data of cells treated with AIDC2*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep1 SRR21924918 AIDC1-BE3-d2GFP-Rep3_R1.fastq.gz HTS data of cells treated with AIDC1*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep3 SRR21924919 AIDC1-BE3-d2GFP-Rep2_R1.fastq.gz HTS data of cells treated with AIDC1*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep2 SRR21924920 AIDC1-BE3-d2GFP-Rep1_R1.fastq.gz HTS data of cells treated with AIDC1*-BE3 base editor and d2GFP sgRNA, Figure 2-Rep1 SRR21924921 Untreated_d2GFP_R1.fastq.gz HTS data of cells untreated with base editor corresponding to d2GFP site SRR21924922 Untreated_CDA-TDG_g1-TDG_g2-IDH2-FANCF_R1.fastq.gz HTS data of cells untreated with base editor corresponding to CDA, TDG_g1, TDG_g2, IDH2 and FANCF sites