SRX17919162 CP-2021-S25 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434812 16S-CP-2021-P8-NA-c_S25 CP-2021-S25 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919163 CP-2019-S146 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434813 CP-16S-Sphagnum-2019-P13-C-a_S146 CP-2019-S146 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919164 CP-2019-S162 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434814 CP-16S-Sphagnum-2019-P13-C-b_S162 CP-2019-S162 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919165 CP-2019-S148 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434815 CP-16S-Sphagnum-2019-P13-C-c_S148 CP-2019-S148 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919166 CP-2019-S159 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434816 CP-16S-Sphagnum-2019-P15-C-a_S159 CP-2019-S159 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919167 CP-2019-S160 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434817 CP-16S-Sphagnum-2019-P15-C-b_S160 CP-2019-S160 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919168 CP-2019-S161 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434818 CP-16S-Sphagnum-2019-P15-C-c_S161 CP-2019-S161 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919169 CP-2019-S157 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434819 CP-16S-Sphagnum-2019-P17-C-a_S157 CP-2019-S157 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919170 CP-2021-S26 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434820 16S-CP-2021-P10-NA-a_S26 CP-2021-S26 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919171 CP-2021-S27 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434821 16S-CP-2021-P10-NA-b_S27 CP-2021-S27 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919172 CP-2021-S22 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434822 16S-CP-2021-P16-NA-c_S22 CP-2021-S22 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919173 CP-2021-S29 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434823 16S-CP-2021-P17-NA-a_S29 CP-2021-S29 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919174 CP-2021-S30 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434824 16S-CP-2021-P17-NA-b_S30 CP-2021-S30 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919175 CP-2021-S31 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434825 16S-CP-2021-P17-NA-c_S31 CP-2021-S31 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919176 CP-2021-S6 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434826 16S-CP-2021-P19-NA-a_S6 CP-2021-S6 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919177 CP-2021-S7 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434827 16S-CP-2021-P19-NA-b_S7 CP-2021-S7 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919178 CP-2021-S8 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434828 16S-CP-2021-P19-NA-c_S8 CP-2021-S8 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919179 CP-2021-S9 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434829 16S-CP-2021-P20-NA-a_S9 CP-2021-S9 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919180 CP-2021-S10 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434830 16S-CP-2021-P20-NA-b_S10 CP-2021-S10 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919181 CP-2021-S11 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434831 16S-CP-2021-P20-NA-c_S11 CP-2021-S11 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919182 CP-2021-S28 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434832 16S-CP-2021-P10-NA-c_S28 CP-2021-S28 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919183 CP-2021-S14 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434833 16S-CP-2021-P4-NA-a_S14 CP-2021-S14 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919184 CP-2021-S15 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434834 16S-CP-2021-P4-NA-b_S15 CP-2021-S15 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919185 CP-2021-S16 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434835 16S-CP-2021-P4-NA-c_S16 CP-2021-S16 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919186 CP-2019-S152 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434836 CP-16S-Sphagnum-2019-P21-C-c_S152 CP-2019-S152 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919187 CP-2019-S145 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434837 CP-16S-Sphagnum-2019-P4-C-a_S145 CP-2019-S145 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919188 CP-2019-S156 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434838 CP-16S-Sphagnum-2019-P4-C-b_S156 CP-2019-S156 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919189 CP-2019-S147 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434839 CP-16S-Sphagnum-2019-P4-C-c_S147 CP-2019-S147 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919190 CP-2019-S153 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434840 CP-16S-Sphagnum-2019-P6-C-a_S153 CP-2019-S153 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919191 CP-2021-S17 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434841 16S-CP-2021-P13-NA-a_S17 CP-2021-S17 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919192 CP-2019-S142 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434842 CP-16S-Sphagnum-2019-P6-C-b_S142 CP-2019-S142 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919193 CP-2019-S154 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434843 CP-16S-Sphagnum-2019-P6-C-c_S154 CP-2019-S154 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919194 CP-2021-S3 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434844 16S-CP-2021-P6-NA-a_S3 CP-2021-S3 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919195 CP-2021-S4 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434845 16S-CP-2021-P6-NA-b_S4 CP-2021-S4 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919196 CP-2021-S5 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434846 16S-CP-2021-P6-NA-c_S5 CP-2021-S5 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919197 CP-2021-S1 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434847 16S-CP-2021-P7-NA-b_S1 CP-2021-S1 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919198 CP-2021-S2 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434848 16S-CP-2021-P7-NA-c_S2 CP-2021-S2 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919199 CP-2021-S23 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434849 16S-CP-2021-P8-NA-a_S23 CP-2021-S23 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919200 CP-2021-S24 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434850 16S-CP-2021-P8-NA-b_S24 CP-2021-S24 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919201 CP-2021-S12 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434851 16S-CP-2021-P11-NA-a_S12 CP-2021-S12 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919202 CP-2019-S139 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434852 CP-16S-Sphagnum-2019-P7-C-a_S139 CP-2019-S139 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919203 CP-2019-S140 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434853 CP-16S-Sphagnum-2019-P7-C-b_S140 CP-2019-S140 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919204 CP-2019-S150 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434854 CP-16S-Sphagnum-2019-P7-C-c_S150 CP-2019-S150 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919205 CP-2021-S18 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434855 16S-CP-2021-P13-NA-b_S18 CP-2021-S18 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919206 CP-2021-S19 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434856 16S-CP-2021-P13-NA-c_S19 CP-2021-S19 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919207 CP-2021-S20 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434857 16S-CP-2021-P16-NA-a_S20 CP-2021-S20 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919208 CP-2021-S21 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434858 16S-CP-2021-P16-NA-b_S21 CP-2021-S21 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919209 CP-2019-S158 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434859 CP-16S-Sphagnum-2019-P17-C-b_S158 CP-2019-S158 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919210 CP-2019-S149 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434861 CP-16S-Sphagnum-2019-P17-C-c_S149 CP-2019-S149 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919211 CP-2021-S13 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434860 16S-CP-2021-P11-NA-b_S13 CP-2021-S13 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919212 CP-2019-S155 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434862 CP-16S-Sphagnum-2019-P19-C-a_S155 CP-2019-S155 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919213 CP-2019-S143 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434863 CP-16S-Sphagnum-2019-P19-C-b_S143 CP-2019-S143 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919214 CP-2019-S144 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434864 CP-16S-Sphagnum-2019-P19-C-c_S144 CP-2019-S144 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919215 CP-2019-S151 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434865 CP-16S-Sphagnum-2019-P21-C-a_S151 CP-2019-S151 AMPLICON GENOMIC PCR Illumina MiSeq SRX17919216 CP-2019-S141 SRP403069 bp0 Sphagnum individuals were collected from the field and immediately frozen at -20C until processing. Each individual was crushed to a powder in liquid N2, and DNA was extracted from ~100 mg of ground sample using the DNeasy Plant Pro Kit (QIAGEN, Valencia, CA) according to the manufacturers instructions. Amplification of the 16S rRNA gene V4 region was performed using the primers 515F-Y (5-GTGYCAGCMGCCGCGGTAA) and 806R-Apprill (5-GGACTACNVGGGTWTCTAAT) primers. . Each primer was modified to include an Illumina overhang adapter sequence on the 5 end for subsequent barcoding. Reactions were performed in triplicate using of 2.5-ng DNA template in a solution containing 1X HotStarTaq Master Mix (QIAGEN), 0.4 mg/ml bovine serum albumin (BSA), 0.2 M of each primer, and 0.76 M of each mitochondrial (mPNA) and plastid (pPNA) peptide nucleic acid (PNA) clamps. PNA clamps have been shown to reduce plant plastid and mitochondrial DNA amplification in PCR reactions. Triplicate PCR products were pooled together and sequenced on an Illumina MiSeq2000 platform using a 500-cycle v2 sequencing kit (250 paired-end reads) at the Georgia Tech High Throughput DNA Sequencing Core in Atlanta, GA. Before submitting to the SRA, the primer and sequencing adapters were removed using Cutadapt software. SRS15434866 CP-16S-Sphagnum-2019-P21-C-b_S141 CP-2019-S141 AMPLICON GENOMIC PCR Illumina MiSeq