SRX17927608 GSM6652400_r1 SRP403192 PRJNA891631 SRS15445323 GSM6652400 GSM6652400 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA The cranial region from each embryo was harvested for single cell mRNA sequencing using a 10X Genomics platform. Embryos were dissected in PBS, the corresponding tissue (whole embryo for E7.5 and cranial region for E8.25-E9.5) was dissociated with papain, neutralized with FBS, and cells were pelleted and resuspended for partitioning on a 10X chromum controller 10x scRNA - Single Cell 3' v3 NextSeq 2000 SRX17927609 GSM6652401_r1 SRP403192 PRJNA891631 SRS15445324 GSM6652401 GSM6652401 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA The cranial region from each embryo was harvested for single cell mRNA sequencing using a 10X Genomics platform. Embryos were dissected in PBS, the corresponding tissue (whole embryo for E7.5 and cranial region for E8.25-E9.5) was dissociated with papain, neutralized with FBS, and cells were pelleted and resuspended for partitioning on a 10X chromum controller 10x scRNA - Single Cell 3' v3 NextSeq 2000 SRX17927610 GSM6652402_r1 SRP403192 PRJNA891631 SRS15445325 GSM6652402 GSM6652402 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA The cranial region from each embryo was harvested for single cell mRNA sequencing using a 10X Genomics platform. Embryos were dissected in PBS, the corresponding tissue (whole embryo for E7.5 and cranial region for E8.25-E9.5) was dissociated with papain, neutralized with FBS, and cells were pelleted and resuspended for partitioning on a 10X chromum controller 10x scRNA - Single Cell 3' v3 NextSeq 2000