SRX17935818 GSM6655992_r1 SRP403252 PRJNA891715 SRS15453467 GSM6655992 GSM6655992 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000 SRX17935819 GSM6655993_r1 SRP403252 PRJNA891715 SRS15453469 GSM6655993 GSM6655993 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000 SRX17935820 GSM6655994_r1 SRP403252 PRJNA891715 SRS15453468 GSM6655994 GSM6655994 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000 SRX17935821 GSM6655995_r1 SRP403252 PRJNA891715 SRS15453470 GSM6655995 GSM6655995 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000 SRX17935822 GSM6655996_r1 SRP403252 PRJNA891715 SRS15453471 GSM6655996 GSM6655996 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000 SRX17935823 GSM6655997_r1 SRP403252 PRJNA891715 SRS15453472 GSM6655997 GSM6655997 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000 SRX17935824 GSM6655998_r1 SRP403252 PRJNA891715 SRS15453473 GSM6655998 GSM6655998 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000 SRX17935825 GSM6655999_r1 SRP403252 PRJNA891715 SRS15453474 GSM6655999 GSM6655999 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000 SRX17935826 GSM6656000_r1 SRP403252 PRJNA891715 SRS15453475 GSM6656000 GSM6656000 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000 SRX17935827 GSM6656001_r1 SRP403252 PRJNA891715 SRS15453476 GSM6656001 GSM6656001 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000 SRX17935828 GSM6656002_r1 SRP403252 PRJNA891715 SRS15453477 GSM6656002 GSM6656002 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000 SRX17935829 GSM6656003_r1 SRP403252 PRJNA891715 SRS15453478 GSM6656003 GSM6656003 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000 SRX17935830 GSM6656004_r1 SRP403252 PRJNA891715 SRS15453479 GSM6656004 GSM6656004 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000 SRX17935831 GSM6656005_r1 SRP403252 PRJNA891715 SRS15453480 GSM6656005 GSM6656005 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000 SRX17935832 GSM6656006_r1 SRP403252 PRJNA891715 SRS15453481 GSM6656006 GSM6656006 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000 SRX17935833 GSM6656007_r1 SRP403252 PRJNA891715 SRS15453482 GSM6656007 GSM6656007 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000 SRX17935834 GSM6656008_r1 SRP403252 PRJNA891715 SRS15453483 GSM6656008 GSM6656008 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000 SRX17935835 GSM6656009_r1 SRP403252 PRJNA891715 SRS15453484 GSM6656009 GSM6656009 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000 SRX17935836 GSM6656010_r1 SRP403252 PRJNA891715 SRS15453485 GSM6656010 GSM6656010 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000 SRX17935837 GSM6656011_r1 SRP403252 PRJNA891715 SRS15453486 GSM6656011 GSM6656011 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000 SRX17935838 GSM6656012_r1 SRP403252 PRJNA891715 SRS15453487 GSM6656012 GSM6656012 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000 SRX17935839 GSM6656013_r1 SRP403252 PRJNA891715 SRS15453488 GSM6656013 GSM6656013 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000 SRX17935840 GSM6656022_r1 SRP403252 PRJNA891715 SRS15453489 GSM6656022 GSM6656022 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000 SRX17935841 GSM6656023_r1 SRP403252 PRJNA891715 SRS15453490 GSM6656023 GSM6656023 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000 SRX17935842 GSM6656024_r1 SRP403252 PRJNA891715 SRS15453491 GSM6656024 GSM6656024 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000 SRX17935843 GSM6656025_r1 SRP403252 PRJNA891715 SRS15453492 GSM6656025 GSM6656025 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000 SRX17935844 GSM6656026_r1 SRP403252 PRJNA891715 SRS15453493 GSM6656026 GSM6656026 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000 SRX17935845 GSM6656027_r1 SRP403252 PRJNA891715 SRS15453494 GSM6656027 GSM6656027 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000 SRX17935846 GSM6656014_r1 SRP403252 PRJNA891715 SRS15453495 GSM6656014 GSM6656014 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000 SRX17935847 GSM6656015_r1 SRP403252 PRJNA891715 SRS15453496 GSM6656015 GSM6656015 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000 SRX17935848 GSM6656016_r1 SRP403252 PRJNA891715 SRS15453497 GSM6656016 GSM6656016 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000 SRX17935849 GSM6656017_r1 SRP403252 PRJNA891715 SRS15453498 GSM6656017 GSM6656017 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000 SRX17935850 GSM6656018_r1 SRP403252 PRJNA891715 SRS15453499 GSM6656018 GSM6656018 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000 SRX17935851 GSM6656019_r1 SRP403252 PRJNA891715 SRS15453500 GSM6656019 GSM6656019 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000 SRX17935852 GSM6656020_r1 SRP403252 PRJNA891715 SRS15453501 GSM6656020 GSM6656020 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000 SRX17935853 GSM6656021_r1 SRP403252 PRJNA891715 SRS15453502 GSM6656021 GSM6656021 RNA-Seq TRANSCRIPTOMIC cDNA Total mRNA were isolated from mouse kidney and human RPTECs using a RNA kit from Qiagen (RNEASY Mini prep kit, Cat# 74104). Total RNA integrity was determined using Agilent 4200 Tapestation. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq-6000 using paired end reads extending 150 bases. Illumina NovaSeq 6000