SRX17952594 GSM6658034_r1 SRP403423 PRJNA891954 SRS15469799 GSM6658034 GSM6658034 RNA-Seq TRANSCRIPTOMIC cDNA Mice were euthanized by intraperitoneal injection of 20% urethane and the hypothalamus of mice was isolated and snap frozen in liquid nitrogen. RNA was isolated using RNAqueous (AM1914, Ambion) according to the manufacturer's protocol. Dynabeads mRNA DIRECT Micro Kit (Ambion) was used to isolate Intact mRNA. The total mRNA samples were depleted of up to 99.9% of 5S, 5.8S, 18S, and 28S rRNA using the RiboMinus Eukaryote System v2 (Life Technologies, Carlsbad, CA, USA). Barcoded cDNA libraries were constructed from the rRNA-depleted mRNA samples and prepared using the Ion Total-RNA Seq Kit v2 (Life Technologies). Ion Torrent Proton SRX17952595 GSM6658035_r1 SRP403423 PRJNA891954 SRS15469800 GSM6658035 GSM6658035 RNA-Seq TRANSCRIPTOMIC cDNA Mice were euthanized by intraperitoneal injection of 20% urethane and the hypothalamus of mice was isolated and snap frozen in liquid nitrogen. RNA was isolated using RNAqueous (AM1914, Ambion) according to the manufacturer's protocol. Dynabeads mRNA DIRECT Micro Kit (Ambion) was used to isolate Intact mRNA. The total mRNA samples were depleted of up to 99.9% of 5S, 5.8S, 18S, and 28S rRNA using the RiboMinus Eukaryote System v2 (Life Technologies, Carlsbad, CA, USA). Barcoded cDNA libraries were constructed from the rRNA-depleted mRNA samples and prepared using the Ion Total-RNA Seq Kit v2 (Life Technologies). Ion Torrent Proton SRX17952596 GSM6658036_r1 SRP403423 PRJNA891954 SRS15469801 GSM6658036 GSM6658036 RNA-Seq TRANSCRIPTOMIC cDNA Mice were euthanized by intraperitoneal injection of 20% urethane and the hypothalamus of mice was isolated and snap frozen in liquid nitrogen. RNA was isolated using RNAqueous (AM1914, Ambion) according to the manufacturer's protocol. Dynabeads mRNA DIRECT Micro Kit (Ambion) was used to isolate Intact mRNA. The total mRNA samples were depleted of up to 99.9% of 5S, 5.8S, 18S, and 28S rRNA using the RiboMinus Eukaryote System v2 (Life Technologies, Carlsbad, CA, USA). Barcoded cDNA libraries were constructed from the rRNA-depleted mRNA samples and prepared using the Ion Total-RNA Seq Kit v2 (Life Technologies). Ion Torrent Proton SRX17952597 GSM6658037_r1 SRP403423 PRJNA891954 SRS15469802 GSM6658037 GSM6658037 RNA-Seq TRANSCRIPTOMIC cDNA Mice were euthanized by intraperitoneal injection of 20% urethane and the hypothalamus of mice was isolated and snap frozen in liquid nitrogen. RNA was isolated using RNAqueous (AM1914, Ambion) according to the manufacturer's protocol. Dynabeads mRNA DIRECT Micro Kit (Ambion) was used to isolate Intact mRNA. The total mRNA samples were depleted of up to 99.9% of 5S, 5.8S, 18S, and 28S rRNA using the RiboMinus Eukaryote System v2 (Life Technologies, Carlsbad, CA, USA). Barcoded cDNA libraries were constructed from the rRNA-depleted mRNA samples and prepared using the Ion Total-RNA Seq Kit v2 (Life Technologies). Ion Torrent Proton