SRX17971736 GSM6664614_r1 SRP403716 PRJNA892642 SRS15488008 GSM6664614 GSM6664614 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA RNA from all samples was prepared using TriZol reagent followed by Rneasy Mini Kit purification, following manufacturer protocol. RNA purity was confirmed by NanoDrop Spectrophotometry and integrity confirmed by Agilent BioAnalyzer. Only RNA with a RIN > 8.0 was used for sequencing. Libraries were constructed using the 10X Genomics chromium single cell 3' reagent v2 chemistry kit for all placental tissue samples and using the chromium single cell 3' reagent v3 chemistry kit for all trophoblast organoid samples at a read depth of 50,000 reads/cell for undifferentiated and a read depth of 150,000 reads/cell for EVT-differentiated PD-TOrg samples. NextSeq 500 SRX17971737 GSM6664615_r1 SRP403716 PRJNA892642 SRS15488007 GSM6664615 GSM6664615 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA RNA from all samples was prepared using TriZol reagent followed by Rneasy Mini Kit purification, following manufacturer protocol. RNA purity was confirmed by NanoDrop Spectrophotometry and integrity confirmed by Agilent BioAnalyzer. Only RNA with a RIN > 8.0 was used for sequencing. Libraries were constructed using the 10X Genomics chromium single cell 3' reagent v2 chemistry kit for all placental tissue samples and using the chromium single cell 3' reagent v3 chemistry kit for all trophoblast organoid samples at a read depth of 50,000 reads/cell for undifferentiated and a read depth of 150,000 reads/cell for EVT-differentiated PD-TOrg samples. NextSeq 500