SRX17962476 GSM6659280_r1 SRP403578 PRJNA892229 SRS15479034 GSM6659280 GSM6659280 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA On the 22nd day from the first cisplatin/GENUS treatment, samples were anesthetized with isofluoren and cardiac perfused with ice cold DPBS (Thermo Fisher ScientificScienctific, 14190235). Two hippocampi per animal were isolated and four hippocampi from two animals were homogenized using adult neural tissue dissociation kit (Miltenyi bio, 130-092-628). The resulting single cell suspension was processed to generate a cDNA library with targeted recovery of 5000 cells using 10x Genomics Chromium™ Single Cell 3' Library & Gel Bead Kit v3.1 (1000215, 1000268, 1000127). The library was sequenced with NovaSeq 6000 (Illumina) and NovaSeq S4 Flow Cell at MIT bioinformatics core. Illumina NovaSeq 6000 SRX17962477 GSM6659281_r1 SRP403578 PRJNA892229 SRS15479035 GSM6659281 GSM6659281 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA On the 22nd day from the first cisplatin/GENUS treatment, samples were anesthetized with isofluoren and cardiac perfused with ice cold DPBS (Thermo Fisher ScientificScienctific, 14190235). Two hippocampi per animal were isolated and four hippocampi from two animals were homogenized using adult neural tissue dissociation kit (Miltenyi bio, 130-092-628). The resulting single cell suspension was processed to generate a cDNA library with targeted recovery of 5000 cells using 10x Genomics Chromium™ Single Cell 3' Library & Gel Bead Kit v3.1 (1000215, 1000268, 1000127). The library was sequenced with NovaSeq 6000 (Illumina) and NovaSeq S4 Flow Cell at MIT bioinformatics core. Illumina NovaSeq 6000 SRX17962478 GSM6659282_r1 SRP403578 PRJNA892229 SRS15479036 GSM6659282 GSM6659282 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA On the 22nd day from the first cisplatin/GENUS treatment, samples were anesthetized with isofluoren and cardiac perfused with ice cold DPBS (Thermo Fisher ScientificScienctific, 14190235). Two hippocampi per animal were isolated and four hippocampi from two animals were homogenized using adult neural tissue dissociation kit (Miltenyi bio, 130-092-628). The resulting single cell suspension was processed to generate a cDNA library with targeted recovery of 5000 cells using 10x Genomics Chromium™ Single Cell 3' Library & Gel Bead Kit v3.1 (1000215, 1000268, 1000127). The library was sequenced with NovaSeq 6000 (Illumina) and NovaSeq S4 Flow Cell at MIT bioinformatics core. Illumina NovaSeq 6000 SRX17962479 GSM6659283_r1 SRP403578 PRJNA892229 SRS15479037 GSM6659283 GSM6659283 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA On the 22nd day from the first cisplatin/GENUS treatment, samples were anesthetized with isofluoren and cardiac perfused with ice cold DPBS (Thermo Fisher ScientificScienctific, 14190235). Two hippocampi per animal were isolated and four hippocampi from two animals were homogenized using adult neural tissue dissociation kit (Miltenyi bio, 130-092-628). The resulting single cell suspension was processed to generate a cDNA library with targeted recovery of 5000 cells using 10x Genomics Chromium™ Single Cell 3' Library & Gel Bead Kit v3.1 (1000215, 1000268, 1000127). The library was sequenced with NovaSeq 6000 (Illumina) and NovaSeq S4 Flow Cell at MIT bioinformatics core. Illumina NovaSeq 6000 SRX17962480 GSM6659284_r1 SRP403578 PRJNA892229 SRS15479039 GSM6659284 GSM6659284 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA On the 22nd day from the first cisplatin/GENUS treatment, samples were anesthetized with isofluoren and cardiac perfused with ice cold DPBS (Thermo Fisher ScientificScienctific, 14190235). Two hippocampi per animal were isolated and four hippocampi from two animals were homogenized using adult neural tissue dissociation kit (Miltenyi bio, 130-092-628). The resulting single cell suspension was processed to generate a cDNA library with targeted recovery of 5000 cells using 10x Genomics Chromium™ Single Cell 3' Library & Gel Bead Kit v3.1 (1000215, 1000268, 1000127). The library was sequenced with NovaSeq 6000 (Illumina) and NovaSeq S4 Flow Cell at MIT bioinformatics core. Illumina NovaSeq 6000 SRX17962481 GSM6659285_r1 SRP403578 PRJNA892229 SRS15479038 GSM6659285 GSM6659285 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA On the 22nd day from the first cisplatin/GENUS treatment, samples were anesthetized with isofluoren and cardiac perfused with ice cold DPBS (Thermo Fisher ScientificScienctific, 14190235). Two hippocampi per animal were isolated and four hippocampi from two animals were homogenized using adult neural tissue dissociation kit (Miltenyi bio, 130-092-628). The resulting single cell suspension was processed to generate a cDNA library with targeted recovery of 5000 cells using 10x Genomics Chromium™ Single Cell 3' Library & Gel Bead Kit v3.1 (1000215, 1000268, 1000127). The library was sequenced with NovaSeq 6000 (Illumina) and NovaSeq S4 Flow Cell at MIT bioinformatics core. Illumina NovaSeq 6000 SRX17962482 GSM6659286_r1 SRP403578 PRJNA892229 SRS15479040 GSM6659286 GSM6659286 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA On the 22nd day from the first cisplatin/GENUS treatment, samples were anesthetized with isofluoren and cardiac perfused with ice cold DPBS (Thermo Fisher ScientificScienctific, 14190235). Two hippocampi per animal were isolated and four hippocampi from two animals were homogenized using adult neural tissue dissociation kit (Miltenyi bio, 130-092-628). The resulting single cell suspension was processed to generate a cDNA library with targeted recovery of 5000 cells using 10x Genomics Chromium™ Single Cell 3' Library & Gel Bead Kit v3.1 (1000215, 1000268, 1000127). The library was sequenced with NovaSeq 6000 (Illumina) and NovaSeq S4 Flow Cell at MIT bioinformatics core. Illumina NovaSeq 6000 SRX17962483 GSM6659287_r1 SRP403578 PRJNA892229 SRS15479041 GSM6659287 GSM6659287 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA On the 22nd day from the first cisplatin/GENUS treatment, samples were anesthetized with isofluoren and cardiac perfused with ice cold DPBS (Thermo Fisher ScientificScienctific, 14190235). Two hippocampi per animal were isolated and four hippocampi from two animals were homogenized using adult neural tissue dissociation kit (Miltenyi bio, 130-092-628). The resulting single cell suspension was processed to generate a cDNA library with targeted recovery of 5000 cells using 10x Genomics Chromium™ Single Cell 3' Library & Gel Bead Kit v3.1 (1000215, 1000268, 1000127). The library was sequenced with NovaSeq 6000 (Illumina) and NovaSeq S4 Flow Cell at MIT bioinformatics core. Illumina NovaSeq 6000 SRX17962484 GSM6659288_r1 SRP403578 PRJNA892229 SRS15479042 GSM6659288 GSM6659288 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA On the 22nd day from the first cisplatin/GENUS treatment, samples were anesthetized with isofluoren and cardiac perfused with ice cold DPBS (Thermo Fisher ScientificScienctific, 14190235). Two hippocampi per animal were isolated and four hippocampi from two animals were homogenized using adult neural tissue dissociation kit (Miltenyi bio, 130-092-628). The resulting single cell suspension was processed to generate a cDNA library with targeted recovery of 5000 cells using 10x Genomics Chromium™ Single Cell 3' Library & Gel Bead Kit v3.1 (1000215, 1000268, 1000127). The library was sequenced with NovaSeq 6000 (Illumina) and NovaSeq S4 Flow Cell at MIT bioinformatics core. Illumina NovaSeq 6000 SRX17962485 GSM6659289_r1 SRP403578 PRJNA892229 SRS15479043 GSM6659289 GSM6659289 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA On the 22nd day from the first cisplatin/GENUS treatment, samples were anesthetized with isofluoren and cardiac perfused with ice cold DPBS (Thermo Fisher ScientificScienctific, 14190235). Two hippocampi per animal were isolated and four hippocampi from two animals were homogenized using adult neural tissue dissociation kit (Miltenyi bio, 130-092-628). The resulting single cell suspension was processed to generate a cDNA library with targeted recovery of 5000 cells using 10x Genomics Chromium™ Single Cell 3' Library & Gel Bead Kit v3.1 (1000215, 1000268, 1000127). The library was sequenced with NovaSeq 6000 (Illumina) and NovaSeq S4 Flow Cell at MIT bioinformatics core. Illumina NovaSeq 6000 SRX17962486 GSM6659290_r1 SRP403578 PRJNA892229 SRS15479044 GSM6659290 GSM6659290 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA On the 22nd day from the first cisplatin/GENUS treatment, samples were anesthetized with isofluoren and cardiac perfused with ice cold DPBS (Thermo Fisher ScientificScienctific, 14190235). Two hippocampi per animal were isolated and four hippocampi from two animals were homogenized using adult neural tissue dissociation kit (Miltenyi bio, 130-092-628). The resulting single cell suspension was processed to generate a cDNA library with targeted recovery of 5000 cells using 10x Genomics Chromium™ Single Cell 3' Library & Gel Bead Kit v3.1 (1000215, 1000268, 1000127). The library was sequenced with NovaSeq 6000 (Illumina) and NovaSeq S4 Flow Cell at MIT bioinformatics core. Illumina NovaSeq 6000 SRX17962487 GSM6659291_r1 SRP403578 PRJNA892229 SRS15479045 GSM6659291 GSM6659291 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA On the 22nd day from the first cisplatin/GENUS treatment, samples were anesthetized with isofluoren and cardiac perfused with ice cold DPBS (Thermo Fisher ScientificScienctific, 14190235). Two hippocampi per animal were isolated and four hippocampi from two animals were homogenized using adult neural tissue dissociation kit (Miltenyi bio, 130-092-628). The resulting single cell suspension was processed to generate a cDNA library with targeted recovery of 5000 cells using 10x Genomics Chromium™ Single Cell 3' Library & Gel Bead Kit v3.1 (1000215, 1000268, 1000127). The library was sequenced with NovaSeq 6000 (Illumina) and NovaSeq S4 Flow Cell at MIT bioinformatics core. Illumina NovaSeq 6000