SRX18014750 GSM6674754_r1 SRP404414 PRJNA893695 SRS15522999 GSM6674754 GSM6674754 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA The frozen PBMCs were quickly thawed at 37°C and gently resuspended by serial additions of DMEM + 10% heat-inactivated FBS, for a final volume of 14ml. Following centrifugation at 300g for 5 min, cells were washed twice with 5ml DMEM + 10% HI-FBS to remove cell debris. Cell counting and viability assessment was done using the LIVE/DEAD™ Viability kit (ThermoFisher Scientific), according to manufacturer's guidelines. Single-cell library: The samples were loaded onto a 10X Genomics Chromium G chip aiming to capture 10,000 cells per samples. The reverse transcription and library preparation was performed using the Chromium Single Cell 3′ v2 Reagent Kits following manufacturers guidelines. The cDNA library quality was assessed using a TapeStation (Agilent). Sequencing: The cDNA libraries were sequenced on a HiSeq 4000 sequencer. Illumina HiSeq 4000 SRX18014751 GSM6674755_r1 SRP404414 PRJNA893695 SRS15523000 GSM6674755 GSM6674755 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA The frozen PBMCs were quickly thawed at 37°C and gently resuspended by serial additions of DMEM + 10% heat-inactivated FBS, for a final volume of 14ml. Following centrifugation at 300g for 5 min, cells were washed twice with 5ml DMEM + 10% HI-FBS to remove cell debris. Cell counting and viability assessment was done using the LIVE/DEAD™ Viability kit (ThermoFisher Scientific), according to manufacturer's guidelines. Single-cell library: The samples were loaded onto a 10X Genomics Chromium G chip aiming to capture 10,000 cells per samples. The reverse transcription and library preparation was performed using the Chromium Single Cell 3′ v2 Reagent Kits following manufacturers guidelines. The cDNA library quality was assessed using a TapeStation (Agilent). Sequencing: The cDNA libraries were sequenced on a HiSeq 4000 sequencer. Illumina HiSeq 4000