SRS15540319 SAMN31462905 97227 Gomphonema parvulum bioproject 894532 PRJNA894532 strain VA_01 collection_date 2021-05 depth surface env_broad_scale Shallow (<=500cm) stagnant ephemeral pool approximately 200 m2 in size. env_local_scale It is located on a site that had been surface mined for coal in the 1980s and 1990s, reclaimed following the guidelines at that time, and is now part of a preserve managed by the University of Virginias College at Wise. This pool spontaneously formed in a low-lying area and was not a purposefully constructed retention pond. It is bordered along one side by the shrub Eleagnus umbellate, an introduced/invasive species previously used for surface mine reclamation, which provides partial shade. This body of water supports local fauna and amphibian egg clutches are often found there in the early spring. env_medium water geo_loc_name USA: Wise, Virginia isol_growth_condt Water collected from the pool was used to produce Pond Agar medium to culture micro-algae, (0% bacto-agar, pH 7.4, buffered with 00M Tris). Microeukaryotes were collected by centrifuging two liters of the collected water at 3000xg for 15 minutes, pellets were resuspended in a small amount of sterile distilled water, spread onto Pond Agar, and incubated at room temperature with natural light (windowsill). Plates were screened every few days using a dissecting microscope to identify small isolated colonies. Colonies were transferred to new Pond Agar plates using a flame sterilized inoculation loop and/or a lab spatula. Isolates were re-screened and transferred over the course of several weeks to produce culture plates with a single alga. lat_lon 36.58 N 82.33 W altitude 779m BioSampleModel MIGS.eu BioSampleModel MIGS/MIMS/MIMARKS.water