<?xml version="1.0" encoding="UTF-8"?>
<STUDY_SET xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
  <STUDY center_name="GEO" alias="GSE217941" accession="SRP407842">
    <IDENTIFIERS>
      <PRIMARY_ID>SRP407842</PRIMARY_ID>
      <EXTERNAL_ID namespace="BioProject" label="primary">PRJNA901441</EXTERNAL_ID>
      <EXTERNAL_ID namespace="GEO">GSE217941</EXTERNAL_ID>
    </IDENTIFIERS>
    <DESCRIPTOR>
      <STUDY_TITLE>Map active enhancer and gene promoter regions in the SCN</STUDY_TITLE>
      <STUDY_TYPE existing_study_type="Other"/>
      <STUDY_ABSTRACT>Histone ChIP-seq was performed at varied time of the day to map the gene promoter and active enhancer sites in the central clock; SCN (suprachiasmatic nuclei). Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for the histone modifications H3K4me3 and H3K27ac in adult mouse SCN and cortex at distinct time of the day . Overall design: WT C57Bl/6J mice were used for SCN tissue collection at 6 distinct time-points starting from ZT3 at every 4 hours, where lights on at 7 am (ZT0) and lights off at 7 pm (ZT12). We also collected cortical punches at ZT3 and ZT15 to compare and establish SCN enriched chromatin modifications. For histone chromatin immunoprecipitation two separate biological replicates per time-point per tissue-type was collected where each biological replicate composed of 3-4 individual SCN or cortical punches.</STUDY_ABSTRACT>
      <CENTER_PROJECT_NAME>GSE217941</CENTER_PROJECT_NAME>
    </DESCRIPTOR>
    <STUDY_LINKS>
      <STUDY_LINK>
        <XREF_LINK>
          <DB>pubmed</DB>
          <ID>37156620</ID>
        </XREF_LINK>
      </STUDY_LINK>
    </STUDY_LINKS>
    <STUDY_ATTRIBUTES>
      <STUDY_ATTRIBUTE>
        <TAG>parent_bioproject</TAG>
        <VALUE>PRJNA901434</VALUE>
      </STUDY_ATTRIBUTE>
    </STUDY_ATTRIBUTES>
  </STUDY>
</STUDY_SET>
