MNase-Seq of gDNA from Pyrococcus furiosus, exponential growth, replicate 1

SRX18273151 Pfuriosus_MNase_exponential_Rep1 MNase-Seq of gDNA from Pyrococcus furiosus, exponential growth, replicate 1 SRP408047 PRJNA901420 MNase digestion, gel purification, library synthesis by NEBNext Ultra II DNA kit with no fragmentation SRS15833182 SAMN31712712 Pfuriosus_MNase_exponential_Rep1 MNase-Seq GENOMIC RANDOM NextSeq 550 SRX18273152 Pfuriosus_MNase_exponential_Rep2 MNase-Seq of gDNA from Pyrococcus furiosus, exponential growth, replicate 2 SRP408047 PRJNA901420 MNase digestion, gel purification, library synthesis by NEBNext Ultra II DNA kit with no fragmentation SRS15833182 SAMN31712712 Pfuriosus_MNase_exponential_Rep2 MNase-Seq GENOMIC RANDOM NextSeq 550 SRX18273153 pCRISPR7_invitro_histones_tenth_Rep1 Spacer in vitro integration into pCRISPR7 with 1/10 saturating concentration histones A/B, rep 1 SRP408047 PRJNA901420 Amplicon library with one degenerate primer and one spacer-specific primer SRS15833182 SAMN31712712 pCRISPR7_invitro_histones_tenth_Rep1 AMPLICON SYNTHETIC PCR NextSeq 550 SRX18273154 pCRISPR7_invitro_histones_zero_Rep1 Spacer in vitro integration into pCRISPR7 with no histones A/B, rep 1 SRP408047 PRJNA901420 Amplicon library with one degenerate primer and one spacer-specific primer SRS15833182 SAMN31712712 pCRISPR7_invitro_histones_zero_Rep1 AMPLICON SYNTHETIC PCR NextSeq 550 SRX18273155 pCRISPR7_MNase_100 MNase-Seq of pCRISPR7 plasmid with purified histones A and B, 100 units MNase SRP408047 PRJNA901420 MNase digestion, gel purification, library synthesis by NEBNext Ultra II DNA kit with no fragmentation SRS15833182 SAMN31712712 pCRISPR7_MNase_100 MNase-Seq SYNTHETIC RANDOM Illumina MiSeq SRX18273156 pCRISPR7_MNase_500 MNase-Seq of pCRISPR7 plasmid with purified histones A and B, 500 units MNase SRP408047 PRJNA901420 MNase digestion, gel purification, library synthesis by NEBNext Ultra II DNA kit with no fragmentation SRS15833182 SAMN31712712 pCRISPR7_MNase_500 MNase-Seq SYNTHETIC RANDOM Illumina MiSeq SRX18273157 pCRISPR7_invitro_histones_full_Rep1 Spacer in vitro integration into pCRISPR7 with saturating concentration histones A/B, rep 1 SRP408047 PRJNA901420 Amplicon library with one degenerate primer and one spacer-specific primer SRS15833182 SAMN31712712 pCRISPR7_invitro_histones_full_Rep1 AMPLICON SYNTHETIC PCR NextSeq 550 SRX18273158 pCRISPR7_invitro_histones_half_Rep1 Spacer in vitro integration into pCRISPR7 with 1/2 saturating concentration histones A/B, rep 1 SRP408047 PRJNA901420 Amplicon library with one degenerate primer and one spacer-specific primer SRS15833182 SAMN31712712 pCRISPR7_invitro_histones_half_Rep1 AMPLICON SYNTHETIC PCR NextSeq 550 SRX18273159 pCRISPR7_invitro_histones_full_Rep2 Spacer in vitro integration into pCRISPR7 with saturating concentration histones A/B, rep 2 SRP408047 PRJNA901420 Amplicon library with one degenerate primer and one spacer-specific primer SRS15833182 SAMN31712712 pCRISPR7_invitro_histones_full_Rep2 AMPLICON SYNTHETIC PCR NextSeq 550 SRX18273160 pCRISPR7_invitro_histones_half_Rep2 Spacer in vitro integration into pCRISPR7 with 1/2 saturating concentration histones A/B, rep 2 SRP408047 PRJNA901420 Amplicon library with one degenerate primer and one spacer-specific primer SRS15833182 SAMN31712712 pCRISPR7_invitro_histones_half_Rep2 AMPLICON SYNTHETIC PCR NextSeq 550 SRX18273161 pCRISPR7_invitro_histones_tenth_Rep2 Spacer in vitro integration into pCRISPR7 with 1/10 saturating concentration histones A/B, rep 2 SRP408047 PRJNA901420 Amplicon library with one degenerate primer and one spacer-specific primer SRS15833182 SAMN31712712 pCRISPR7_invitro_histones_tenth_Rep2 AMPLICON SYNTHETIC PCR NextSeq 550 SRX18273162 pCRISPR7_invitro_histones_zero_Rep2 Spacer in vitro integration into pCRISPR7 with no histones A/B, rep 2 SRP408047 PRJNA901420 Amplicon library with one degenerate primer and one spacer-specific primer SRS15833182 SAMN31712712 pCRISPR7_invitro_histones_zero_Rep2 AMPLICON SYNTHETIC PCR NextSeq 550 SRX18273163 pCRISPR7_invitro_histones_full_Rep3 Spacer in vitro integration into pCRISPR7 with saturating concentration histones A/B, rep 3 SRP408047 PRJNA901420 Amplicon library with one degenerate primer and one spacer-specific primer SRS15833182 SAMN31712712 pCRISPR7_invitro_histones_full_Rep3 AMPLICON SYNTHETIC PCR NextSeq 550 SRX18273164 pCRISPR7_invitro_histones_half_Rep3 Spacer in vitro integration into pCRISPR7 with 1/2 saturating concentration histones A/B, rep 3 SRP408047 PRJNA901420 Amplicon library with one degenerate primer and one spacer-specific primer SRS15833182 SAMN31712712 pCRISPR7_invitro_histones_half_Rep3 AMPLICON SYNTHETIC PCR NextSeq 550 SRX18273165 pCRISPR7_invitro_histones_tenth_Rep3 Spacer in vitro integration into pCRISPR7 with 1/10 saturating concentration histones A/B, rep 3 SRP408047 PRJNA901420 Amplicon library with one degenerate primer and one spacer-specific primer SRS15833182 SAMN31712712 pCRISPR7_invitro_histones_tenth_Rep3 AMPLICON SYNTHETIC PCR NextSeq 550 SRX18273166 pCRISPR7_invitro_histones_zero_Rep3 Spacer in vitro integration into pCRISPR7 with no histones A/B, rep 3 SRP408047 PRJNA901420 Amplicon library with one degenerate primer and one spacer-specific primer SRS15833182 SAMN31712712 pCRISPR7_invitro_histones_zero_Rep3 AMPLICON SYNTHETIC PCR NextSeq 550 SRX18273167 Pfuriosus_MNase_exponential_Rep3 MNase-Seq of gDNA from Pyrococcus furiosus, exponential growth, replicate 3 SRP408047 PRJNA901420 MNase digestion, gel purification, library synthesis by NEBNext Ultra II DNA kit with no fragmentation SRS15833182 SAMN31712712 Pfuriosus_MNase_exponential_Rep3 MNase-Seq GENOMIC RANDOM NextSeq 550 SRX18273168 pCRISPR7_invitro_histones_full_Rep4 Spacer in vitro integration into pCRISPR7 with saturating concentration histones A/B, rep 4 SRP408047 PRJNA901420 Amplicon library with one degenerate primer and one spacer-specific primer SRS15833182 SAMN31712712 pCRISPR7_invitro_histones_full_Rep4 AMPLICON SYNTHETIC PCR NextSeq 550 SRX18273169 pCRISPR7_invitro_histones_half_Rep4 Spacer in vitro integration into pCRISPR7 with 1/2 saturating concentration histones A/B, rep 4 SRP408047 PRJNA901420 Amplicon library with one degenerate primer and one spacer-specific primer SRS15833182 SAMN31712712 pCRISPR7_invitro_histones_half_Rep4 AMPLICON SYNTHETIC PCR NextSeq 550 SRX18273170 pCRISPR7_invitro_histones_tenth_Rep4 Spacer in vitro integration into pCRISPR7 with 1/10 saturating concentration histones A/B, rep 4 SRP408047 PRJNA901420 Amplicon library with one degenerate primer and one spacer-specific primer SRS15833182 SAMN31712712 pCRISPR7_invitro_histones_tenth_Rep4 AMPLICON SYNTHETIC PCR NextSeq 550 SRX18273171 pCRISPR7_invitro_histones_zero_Rep4 Spacer in vitro integration into pCRISPR7 with no histones A/B, rep 4 SRP408047 PRJNA901420 Amplicon library with one degenerate primer and one spacer-specific primer SRS15833182 SAMN31712712 pCRISPR7_invitro_histones_zero_Rep4 AMPLICON SYNTHETIC PCR NextSeq 550 SRX18273172 pCRISPR7_invitro_histones_full_Rep5 Spacer in vitro integration into pCRISPR7 with saturating concentration histones A/B, rep 5 SRP408047 PRJNA901420 Amplicon library with one degenerate primer and one spacer-specific primer SRS15833182 SAMN31712712 pCRISPR7_invitro_histones_full_Rep5 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX18273173 pCRISPR7_invitro_histones_half_Rep5 Spacer in vitro integration into pCRISPR7 with 1/2 saturating concentration histones A/B, rep 5 SRP408047 PRJNA901420 Amplicon library with one degenerate primer and one spacer-specific primer SRS15833182 SAMN31712712 pCRISPR7_invitro_histones_half_Rep5 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX18273174 pCRISPR7_invitro_histones_tenth_Rep5 Spacer in vitro integration into pCRISPR7 with 1/10 saturating concentration histones A/B, rep 5 SRP408047 PRJNA901420 Amplicon library with one degenerate primer and one spacer-specific primer SRS15833182 SAMN31712712 pCRISPR7_invitro_histones_tenth_Rep5 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX18273175 pCRISPR7_invitro_histones_zero_Rep5 Spacer in vitro integration into pCRISPR7 with no histones A/B, rep 5 SRP408047 PRJNA901420 Amplicon library with one degenerate primer and one spacer-specific primer SRS15833182 SAMN31712712 pCRISPR7_invitro_histones_zero_Rep5 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX18273176 pCRISPR7_invitro_histones_full_Rep6 Spacer in vitro integration into pCRISPR7 with saturating concentration histones A/B, rep 6 SRP408047 PRJNA901420 Amplicon library with one degenerate primer and one spacer-specific primer SRS15833182 SAMN31712712 pCRISPR7_invitro_histones_full_Rep6 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX18273177 pCRISPR7_invitro_histones_half_Rep6 Spacer in vitro integration into pCRISPR7 with 1/2 saturating concentration histones A/B, rep 6 SRP408047 PRJNA901420 Amplicon library with one degenerate primer and one spacer-specific primer SRS15833182 SAMN31712712 pCRISPR7_invitro_histones_half_Rep6 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX18273178 Pfuriosus_MNase_stationary_Rep1 MNase-Seq of gDNA from Pyrococcus furiosus, stationary growth, replicate 1 SRP408047 PRJNA901420 MNase digestion, gel purification, library synthesis by NEBNext Ultra II DNA kit with no fragmentation SRS15833182 SAMN31712712 Pfuriosus_MNase_stationary_Rep1 MNase-Seq GENOMIC RANDOM NextSeq 550 SRX18273179 pCRISPR7_invitro_histones_tenth_Rep6 Spacer in vitro integration into pCRISPR7 with 1/10 saturating concentration histones A/B, rep 6 SRP408047 PRJNA901420 Amplicon library with one degenerate primer and one spacer-specific primer SRS15833182 SAMN31712712 pCRISPR7_invitro_histones_tenth_Rep6 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX18273180 pCRISPR7_invitro_histones_zero_Rep6 Spacer in vitro integration into pCRISPR7 with no histones A/B, rep 6 SRP408047 PRJNA901420 Amplicon library with one degenerate primer and one spacer-specific primer SRS15833182 SAMN31712712 pCRISPR7_invitro_histones_zero_Rep6 AMPLICON SYNTHETIC PCR Illumina MiSeq SRX18273181 pCRISPR7_invitro_Alba_full Spacer in vitro integration into pCRISPR7 with saturating concentration Alba SRP408047 PRJNA901420 Amplicon library with one degenerate primer and one spacer-specific primer SRS15833182 SAMN31712712 pCRISPR7_invitro_Alba_full AMPLICON SYNTHETIC PCR Illumina MiSeq SRX18273182 pCRISPR7_invitro_Alba_half Spacer in vitro integration into pCRISPR7 with 1/2 saturating concentration Alba SRP408047 PRJNA901420 Amplicon library with one degenerate primer and one spacer-specific primer SRS15833182 SAMN31712712 pCRISPR7_invitro_Alba_half AMPLICON SYNTHETIC PCR Illumina MiSeq SRX18273183 pCRISPR7_invitro_Alba_tenth Spacer in vitro integration into pCRISPR7 with 1/10 saturating concentration Alba SRP408047 PRJNA901420 Amplicon library with one degenerate primer and one spacer-specific primer SRS15833182 SAMN31712712 pCRISPR7_invitro_Alba_tenth AMPLICON SYNTHETIC PCR Illumina MiSeq SRX18273184 pCRISPR7_invitro_Alba_zero Spacer in vitro integration into pCRISPR7 with no Alba SRP408047 PRJNA901420 Amplicon library with one degenerate primer and one spacer-specific primer SRS15833182 SAMN31712712 pCRISPR7_invitro_Alba_zero AMPLICON SYNTHETIC PCR Illumina MiSeq SRX18273185 Pfuriosus_MNase_stationary_Rep2 MNase-Seq of gDNA from Pyrococcus furiosus, stationary growth, replicate 2 SRP408047 PRJNA901420 MNase digestion, gel purification, library synthesis by NEBNext Ultra II DNA kit with no fragmentation SRS15833182 SAMN31712712 Pfuriosus_MNase_stationary_Rep2 MNase-Seq GENOMIC RANDOM NextSeq 550 SRX18273186 Pfuriosus_MNase_stationary_Rep3 MNase-Seq of gDNA from Pyrococcus furiosus, stationary growth, replicate 3 SRP408047 PRJNA901420 MNase digestion, gel purification, library synthesis by NEBNext Ultra II DNA kit with no fragmentation SRS15833182 SAMN31712712 Pfuriosus_MNase_stationary_Rep3 MNase-Seq GENOMIC RANDOM NextSeq 550