GSM6732454: Patient 3 CD8+ [Pt03_CD8 RNA-seq]; Homo sapiens; RNA-Seq

SRX18273072 GSM6732454_r1 GSM6732454: Patient 3 CD8+ [Pt03_CD8 RNA-seq]; Homo sapiens; RNA-Seq SRP408054 PRJNA901910 SRS15765561 GSM6732454 GSM6732454 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from CD4+ and CD8+ sorted TIL with the Qiagen RNEasy Mini Kit. 100ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol (NuGEN Technologies, Inc., San Carlos, CA). These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample. NextSeq 500 SRX18273073 GSM6732455_r1 GSM6732455: Patient 7 CD4+ [Pt07_CD4 RNA-seq]; Homo sapiens; RNA-Seq SRP408054 PRJNA901910 SRS15765562 GSM6732455 GSM6732455 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from CD4+ and CD8+ sorted TIL with the Qiagen RNEasy Mini Kit. 100ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol (NuGEN Technologies, Inc., San Carlos, CA). These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample. NextSeq 500 SRX18273074 GSM6732480_r1 GSM6732480: Patient 21 CD8+ [Pt21_CD8 RNA-seq]; Homo sapiens; RNA-Seq SRP408054 PRJNA901910 SRS15765563 GSM6732480 GSM6732480 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from CD4+ and CD8+ sorted TIL with the Qiagen RNEasy Mini Kit. 100ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol (NuGEN Technologies, Inc., San Carlos, CA). These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample. NextSeq 500 SRX18273075 GSM6732481_r1 GSM6732481: Patient 21 CD4+CD8+ [Pt21_DP RNA-seq]; Homo sapiens; RNA-Seq SRP408054 PRJNA901910 SRS15765564 GSM6732481 GSM6732481 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from CD4+ and CD8+ sorted TIL with the Qiagen RNEasy Mini Kit. 100ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol (NuGEN Technologies, Inc., San Carlos, CA). These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample. NextSeq 500 SRX18273076 GSM6732482_r1 GSM6732482: Patient 22 CD4+ [Pt22_CD4 RNA-seq]; Homo sapiens; RNA-Seq SRP408054 PRJNA901910 SRS15765565 GSM6732482 GSM6732482 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from CD4+ and CD8+ sorted TIL with the Qiagen RNEasy Mini Kit. 100ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol (NuGEN Technologies, Inc., San Carlos, CA). These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample. NextSeq 500 SRX18273077 GSM6732483_r1 GSM6732483: Patient 22 CD8+ [Pt22_CD8 RNA-seq]; Homo sapiens; RNA-Seq SRP408054 PRJNA901910 SRS15765566 GSM6732483 GSM6732483 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from CD4+ and CD8+ sorted TIL with the Qiagen RNEasy Mini Kit. 100ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol (NuGEN Technologies, Inc., San Carlos, CA). These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample. NextSeq 500 SRX18273078 GSM6732484_r1 GSM6732484: Patient 22 CD4+CD8+ [Pt22_DP RNA-seq]; Homo sapiens; RNA-Seq SRP408054 PRJNA901910 SRS15765567 GSM6732484 GSM6732484 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from CD4+ and CD8+ sorted TIL with the Qiagen RNEasy Mini Kit. 100ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol (NuGEN Technologies, Inc., San Carlos, CA). These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample. NextSeq 500 SRX18273079 GSM6732485_r1 GSM6732485: Patient 23 CD4+ [Pt23_CD4 RNA-seq]; Homo sapiens; RNA-Seq SRP408054 PRJNA901910 SRS15765568 GSM6732485 GSM6732485 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from CD4+ and CD8+ sorted TIL with the Qiagen RNEasy Mini Kit. 100ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol (NuGEN Technologies, Inc., San Carlos, CA). These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample. NextSeq 500 SRX18273080 GSM6732487_r1 GSM6732487: Patient 24 CD8+ [Pt24_CD8 RNA-seq]; Homo sapiens; RNA-Seq SRP408054 PRJNA901910 SRS15765569 GSM6732487 GSM6732487 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from CD4+ and CD8+ sorted TIL with the Qiagen RNEasy Mini Kit. 100ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol (NuGEN Technologies, Inc., San Carlos, CA). These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample. NextSeq 500 SRX18273081 GSM6732486_r1 GSM6732486: Patient 24 CD4+ [Pt24_CD4 RNA-seq]; Homo sapiens; RNA-Seq SRP408054 PRJNA901910 SRS15765570 GSM6732486 GSM6732486 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from CD4+ and CD8+ sorted TIL with the Qiagen RNEasy Mini Kit. 100ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol (NuGEN Technologies, Inc., San Carlos, CA). These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample. NextSeq 500 SRX18273082 GSM6732456_r1 GSM6732456: Patient 7 CD8+ [Pt07_CD8 RNA-seq]; Homo sapiens; RNA-Seq SRP408054 PRJNA901910 SRS15765571 GSM6732456 GSM6732456 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from CD4+ and CD8+ sorted TIL with the Qiagen RNEasy Mini Kit. 100ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol (NuGEN Technologies, Inc., San Carlos, CA). These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample. NextSeq 500 SRX18273083 GSM6732457_r1 GSM6732457: Patient 7 CD4+CD8+ [Pt07_DP RNA-seq]; Homo sapiens; RNA-Seq SRP408054 PRJNA901910 SRS15765572 GSM6732457 GSM6732457 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from CD4+ and CD8+ sorted TIL with the Qiagen RNEasy Mini Kit. 100ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol (NuGEN Technologies, Inc., San Carlos, CA). These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample. NextSeq 500 SRX18273084 GSM6732458_r1 GSM6732458: Patient 8 CD4+ [Pt08_CD4 RNA-seq]; Homo sapiens; RNA-Seq SRP408054 PRJNA901910 SRS15765573 GSM6732458 GSM6732458 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from CD4+ and CD8+ sorted TIL with the Qiagen RNEasy Mini Kit. 100ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol (NuGEN Technologies, Inc., San Carlos, CA). These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample. NextSeq 500 SRX18273085 GSM6732459_r1 GSM6732459: Patient 8 CD4+CD8+ [Pt08_DP RNA-seq]; Homo sapiens; RNA-Seq SRP408054 PRJNA901910 SRS15765574 GSM6732459 GSM6732459 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from CD4+ and CD8+ sorted TIL with the Qiagen RNEasy Mini Kit. 100ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol (NuGEN Technologies, Inc., San Carlos, CA). These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample. NextSeq 500 SRX18273086 GSM6732460_r1 GSM6732460: Patient 9 CD4+ [Pt09_CD4 RNA-seq]; Homo sapiens; RNA-Seq SRP408054 PRJNA901910 SRS15765575 GSM6732460 GSM6732460 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from CD4+ and CD8+ sorted TIL with the Qiagen RNEasy Mini Kit. 100ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol (NuGEN Technologies, Inc., San Carlos, CA). These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample. NextSeq 500 SRX18273087 GSM6732461_r1 GSM6732461: Patient 9 CD8+ [Pt09_CD8 RNA-seq]; Homo sapiens; RNA-Seq SRP408054 PRJNA901910 SRS15765576 GSM6732461 GSM6732461 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from CD4+ and CD8+ sorted TIL with the Qiagen RNEasy Mini Kit. 100ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol (NuGEN Technologies, Inc., San Carlos, CA). These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample. NextSeq 500 SRX18273088 GSM6732462_r1 GSM6732462: Patient 9 CD4+CD8+ [Pt09_DP RNA-seq]; Homo sapiens; RNA-Seq SRP408054 PRJNA901910 SRS15765577 GSM6732462 GSM6732462 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from CD4+ and CD8+ sorted TIL with the Qiagen RNEasy Mini Kit. 100ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol (NuGEN Technologies, Inc., San Carlos, CA). These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample. NextSeq 500 SRX18273089 GSM6732463_r1 GSM6732463: Patient 11 CD4+ [Pt11_CD4 RNA-seq]; Homo sapiens; RNA-Seq SRP408054 PRJNA901910 SRS15765578 GSM6732463 GSM6732463 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from CD4+ and CD8+ sorted TIL with the Qiagen RNEasy Mini Kit. 100ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol (NuGEN Technologies, Inc., San Carlos, CA). These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample. NextSeq 500 SRX18273090 GSM6732464_r1 GSM6732464: Patient 11 CD8+ [Pt11_CD8 RNA-seq]; Homo sapiens; RNA-Seq SRP408054 PRJNA901910 SRS15765579 GSM6732464 GSM6732464 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from CD4+ and CD8+ sorted TIL with the Qiagen RNEasy Mini Kit. 100ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol (NuGEN Technologies, Inc., San Carlos, CA). These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample. NextSeq 500 SRX18273091 GSM6732465_r1 GSM6732465: Patient 11 CD4+CD8+ [Pt11_DP RNA-seq]; Homo sapiens; RNA-Seq SRP408054 PRJNA901910 SRS15765580 GSM6732465 GSM6732465 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from CD4+ and CD8+ sorted TIL with the Qiagen RNEasy Mini Kit. 100ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol (NuGEN Technologies, Inc., San Carlos, CA). These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample. NextSeq 500 SRX18273092 GSM6732466_r1 GSM6732466: Patient 14 CD4+ [Pt14_CD4 RNA-seq]; Homo sapiens; RNA-Seq SRP408054 PRJNA901910 SRS15765581 GSM6732466 GSM6732466 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from CD4+ and CD8+ sorted TIL with the Qiagen RNEasy Mini Kit. 100ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol (NuGEN Technologies, Inc., San Carlos, CA). These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample. NextSeq 500 SRX18273093 GSM6732467_r1 GSM6732467: Patient 14 CD8+ [Pt14_CD8 RNA-seq]; Homo sapiens; RNA-Seq SRP408054 PRJNA901910 SRS15765582 GSM6732467 GSM6732467 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from CD4+ and CD8+ sorted TIL with the Qiagen RNEasy Mini Kit. 100ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol (NuGEN Technologies, Inc., San Carlos, CA). These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample. NextSeq 500 SRX18273094 GSM6732468_r1 GSM6732468: Patient 15 CD4+ [Pt15_CD4 RNA-seq]; Homo sapiens; RNA-Seq SRP408054 PRJNA901910 SRS15765583 GSM6732468 GSM6732468 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from CD4+ and CD8+ sorted TIL with the Qiagen RNEasy Mini Kit. 100ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol (NuGEN Technologies, Inc., San Carlos, CA). These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample. NextSeq 500 SRX18273095 GSM6732469_r1 GSM6732469: Patient 15 CD4+CD8+ [Pt15_DP RNA-seq]; Homo sapiens; RNA-Seq SRP408054 PRJNA901910 SRS15765584 GSM6732469 GSM6732469 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from CD4+ and CD8+ sorted TIL with the Qiagen RNEasy Mini Kit. 100ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol (NuGEN Technologies, Inc., San Carlos, CA). These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample. NextSeq 500 SRX18273096 GSM6732470_r1 GSM6732470: Patient 16 CD4+ [Pt16_CD4 RNA-seq]; Homo sapiens; RNA-Seq SRP408054 PRJNA901910 SRS15765585 GSM6732470 GSM6732470 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from CD4+ and CD8+ sorted TIL with the Qiagen RNEasy Mini Kit. 100ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol (NuGEN Technologies, Inc., San Carlos, CA). These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample. NextSeq 500 SRX18273097 GSM6732471_r1 GSM6732471: Patient 16 CD8+ [Pt16_CD8 RNA-seq]; Homo sapiens; RNA-Seq SRP408054 PRJNA901910 SRS15765586 GSM6732471 GSM6732471 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from CD4+ and CD8+ sorted TIL with the Qiagen RNEasy Mini Kit. 100ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol (NuGEN Technologies, Inc., San Carlos, CA). These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample. NextSeq 500 SRX18273098 GSM6732472_r1 GSM6732472: Patient 16 CD4+CD8+ [Pt16_DP RNA-seq]; Homo sapiens; RNA-Seq SRP408054 PRJNA901910 SRS15765587 GSM6732472 GSM6732472 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from CD4+ and CD8+ sorted TIL with the Qiagen RNEasy Mini Kit. 100ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol (NuGEN Technologies, Inc., San Carlos, CA). These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample. NextSeq 500 SRX18273099 GSM6732473_r1 GSM6732473: Patient 18 CD4+ [Pt18_CD4 RNA-seq]; Homo sapiens; RNA-Seq SRP408054 PRJNA901910 SRS15765588 GSM6732473 GSM6732473 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from CD4+ and CD8+ sorted TIL with the Qiagen RNEasy Mini Kit. 100ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol (NuGEN Technologies, Inc., San Carlos, CA). These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample. NextSeq 500 SRX18273100 GSM6732474_r1 GSM6732474: Patient 18 CD8+ [Pt18_CD8 RNA-seq]; Homo sapiens; RNA-Seq SRP408054 PRJNA901910 SRS15765589 GSM6732474 GSM6732474 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from CD4+ and CD8+ sorted TIL with the Qiagen RNEasy Mini Kit. 100ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol (NuGEN Technologies, Inc., San Carlos, CA). These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample. NextSeq 500 SRX18273101 GSM6732475_r1 GSM6732475: Patient 18 CD4+CD8+ [Pt18_DP RNA-seq]; Homo sapiens; RNA-Seq SRP408054 PRJNA901910 SRS15765590 GSM6732475 GSM6732475 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from CD4+ and CD8+ sorted TIL with the Qiagen RNEasy Mini Kit. 100ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol (NuGEN Technologies, Inc., San Carlos, CA). These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample. NextSeq 500 SRX18273102 GSM6732476_r1 GSM6732476: Patient 20 CD4+ [Pt20_CD4 RNA-seq]; Homo sapiens; RNA-Seq SRP408054 PRJNA901910 SRS15765591 GSM6732476 GSM6732476 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from CD4+ and CD8+ sorted TIL with the Qiagen RNEasy Mini Kit. 100ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol (NuGEN Technologies, Inc., San Carlos, CA). These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample. NextSeq 500 SRX18273103 GSM6732477_r1 GSM6732477: Patient 20 CD8+ [Pt20_CD8 RNA-seq]; Homo sapiens; RNA-Seq SRP408054 PRJNA901910 SRS15765592 GSM6732477 GSM6732477 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from CD4+ and CD8+ sorted TIL with the Qiagen RNEasy Mini Kit. 100ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol (NuGEN Technologies, Inc., San Carlos, CA). These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample. NextSeq 500 SRX18273104 GSM6732478_r1 GSM6732478: Patient 20 CD4+CD8+ [Pt20_DP RNA-seq]; Homo sapiens; RNA-Seq SRP408054 PRJNA901910 SRS15765593 GSM6732478 GSM6732478 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from CD4+ and CD8+ sorted TIL with the Qiagen RNEasy Mini Kit. 100ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol (NuGEN Technologies, Inc., San Carlos, CA). These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample. NextSeq 500 SRX18273105 GSM6732479_r1 GSM6732479: Patient 21 CD4+ [Pt21_CD4 RNA-seq]; Homo sapiens; RNA-Seq SRP408054 PRJNA901910 SRS15765594 GSM6732479 GSM6732479 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from CD4+ and CD8+ sorted TIL with the Qiagen RNEasy Mini Kit. 100ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol (NuGEN Technologies, Inc., San Carlos, CA). These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample. NextSeq 500