SRX18289691 GSM6735356_r1 SRP408310 PRJNA902397 SRS15781427 GSM6735356 GSM6735356 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Livers (> 0.5g) were chopped in cold DMEM (Gibco) and digested with the liver dissociation kit, mouse (Miltenyi Biotec) in DMEM according to the manufacturer's instructions. Samples were dissociated with the gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec) as per the default manufacturers protocol for murine liver digestion. The single cell suspension was filtered through a 70 µM mesh strainer and washed with cold DMEM, resuspended in RBC lysis buffer (eBioscience) then washed twice in autoMACs buffer with BSA (Miltenyi Biotec). CD45+ cells were isolated with CD45 microbeads (Miltenyi Biotec) according to the manufacturer's protocol with the autoMACs separator. The single cell suspension was prepared according to the 10X Genomics single cell sample prep protocol. 6000-8000 cells per sample were loaded onto the 10X Chromium Controller RNA-seq libraries were prepared using Chromium Single Cell 5' v2 Reagent Kits (10X Genomics). libraries were sequenced by UCSD IGM Center on an Illumina NGS HiSeq 4000, with 2x 75 paired-end kits. Illumina HiSeq 4000 SRX18289692 GSM6735357_r1 SRP408310 PRJNA902397 SRS15781428 GSM6735357 GSM6735357 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Livers (> 0.5g) were chopped in cold DMEM (Gibco) and digested with the liver dissociation kit, mouse (Miltenyi Biotec) in DMEM according to the manufacturer's instructions. Samples were dissociated with the gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec) as per the default manufacturers protocol for murine liver digestion. The single cell suspension was filtered through a 70 µM mesh strainer and washed with cold DMEM, resuspended in RBC lysis buffer (eBioscience) then washed twice in autoMACs buffer with BSA (Miltenyi Biotec). CD45+ cells were isolated with CD45 microbeads (Miltenyi Biotec) according to the manufacturer's protocol with the autoMACs separator. The single cell suspension was prepared according to the 10X Genomics single cell sample prep protocol. 6000-8000 cells per sample were loaded onto the 10X Chromium Controller RNA-seq libraries were prepared using Chromium Single Cell 5' v2 Reagent Kits (10X Genomics). libraries were sequenced by UCSD IGM Center on an Illumina NGS HiSeq 4000, with 2x 75 paired-end kits. Illumina HiSeq 4000 SRX18289693 GSM6735358_r1 SRP408310 PRJNA902397 SRS15781429 GSM6735358 GSM6735358 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Livers (> 0.5g) were chopped in cold DMEM (Gibco) and digested with the liver dissociation kit, mouse (Miltenyi Biotec) in DMEM according to the manufacturer's instructions. Samples were dissociated with the gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec) as per the default manufacturers protocol for murine liver digestion. The single cell suspension was filtered through a 70 µM mesh strainer and washed with cold DMEM, resuspended in RBC lysis buffer (eBioscience) then washed twice in autoMACs buffer with BSA (Miltenyi Biotec). CD45+ cells were isolated with CD45 microbeads (Miltenyi Biotec) according to the manufacturer's protocol with the autoMACs separator. The single cell suspension was prepared according to the 10X Genomics single cell sample prep protocol. 6000-8000 cells per sample were loaded onto the 10X Chromium Controller RNA-seq libraries were prepared using Chromium Single Cell 5' v2 Reagent Kits (10X Genomics). libraries were sequenced by UCSD IGM Center on an Illumina NGS HiSeq 4000, with 2x 75 paired-end kits. Illumina HiSeq 4000 SRX18289694 GSM6735359_r1 SRP408310 PRJNA902397 SRS15781430 GSM6735359 GSM6735359 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Livers (> 0.5g) were chopped in cold DMEM (Gibco) and digested with the liver dissociation kit, mouse (Miltenyi Biotec) in DMEM according to the manufacturer's instructions. Samples were dissociated with the gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec) as per the default manufacturers protocol for murine liver digestion. The single cell suspension was filtered through a 70 µM mesh strainer and washed with cold DMEM, resuspended in RBC lysis buffer (eBioscience) then washed twice in autoMACs buffer with BSA (Miltenyi Biotec). CD45+ cells were isolated with CD45 microbeads (Miltenyi Biotec) according to the manufacturer's protocol with the autoMACs separator. The single cell suspension was prepared according to the 10X Genomics single cell sample prep protocol. 6000-8000 cells per sample were loaded onto the 10X Chromium Controller RNA-seq libraries were prepared using Chromium Single Cell 5' v2 Reagent Kits (10X Genomics). libraries were sequenced by UCSD IGM Center on an Illumina NGS HiSeq 4000, with 2x 75 paired-end kits. Illumina HiSeq 4000 SRX18289695 GSM6735360_r1 SRP408310 PRJNA902397 SRS15781431 GSM6735360 GSM6735360 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Livers (> 0.5g) were chopped in cold DMEM (Gibco) and digested with the liver dissociation kit, mouse (Miltenyi Biotec) in DMEM according to the manufacturer's instructions. Samples were dissociated with the gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec) as per the default manufacturers protocol for murine liver digestion. The single cell suspension was filtered through a 70 µM mesh strainer and washed with cold DMEM, resuspended in RBC lysis buffer (eBioscience) then washed twice in autoMACs buffer with BSA (Miltenyi Biotec). CD45+ cells were isolated with CD45 microbeads (Miltenyi Biotec) according to the manufacturer's protocol with the autoMACs separator. The single cell suspension was prepared according to the 10X Genomics single cell sample prep protocol. 6000-8000 cells per sample were loaded onto the 10X Chromium Controller RNA-seq libraries were prepared using Chromium Single Cell 5' v2 Reagent Kits (10X Genomics). libraries were sequenced by UCSD IGM Center on an Illumina NGS HiSeq 4000, with 2x 75 paired-end kits. Illumina HiSeq 4000 SRX18289696 GSM6735361_r1 SRP408310 PRJNA902397 SRS15781432 GSM6735361 GSM6735361 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Livers (> 0.5g) were chopped in cold DMEM (Gibco) and digested with the liver dissociation kit, mouse (Miltenyi Biotec) in DMEM according to the manufacturer's instructions. Samples were dissociated with the gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec) as per the default manufacturers protocol for murine liver digestion. The single cell suspension was filtered through a 70 µM mesh strainer and washed with cold DMEM, resuspended in RBC lysis buffer (eBioscience) then washed twice in autoMACs buffer with BSA (Miltenyi Biotec). CD45+ cells were isolated with CD45 microbeads (Miltenyi Biotec) according to the manufacturer's protocol with the autoMACs separator. The single cell suspension was prepared according to the 10X Genomics single cell sample prep protocol. 6000-8000 cells per sample were loaded onto the 10X Chromium Controller RNA-seq libraries were prepared using Chromium Single Cell 5' v2 Reagent Kits (10X Genomics). libraries were sequenced by UCSD IGM Center on an Illumina NGS HiSeq 4000, with 2x 75 paired-end kits. Illumina HiSeq 4000 SRX18289697 GSM6735362_r1 SRP408310 PRJNA902397 SRS15781433 GSM6735362 GSM6735362 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Livers (> 0.5g) were chopped in cold DMEM (Gibco) and digested with the liver dissociation kit, mouse (Miltenyi Biotec) in DMEM according to the manufacturer's instructions. Samples were dissociated with the gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec) as per the default manufacturers protocol for murine liver digestion. The single cell suspension was filtered through a 70 µM mesh strainer and washed with cold DMEM, resuspended in RBC lysis buffer (eBioscience) then washed twice in autoMACs buffer with BSA (Miltenyi Biotec). CD45+ cells were isolated with CD45 microbeads (Miltenyi Biotec) according to the manufacturer's protocol with the autoMACs separator. The single cell suspension was prepared according to the 10X Genomics single cell sample prep protocol. 6000-8000 cells per sample were loaded onto the 10X Chromium Controller RNA-seq libraries were prepared using Chromium Single Cell 5' v2 Reagent Kits (10X Genomics). libraries were sequenced by UCSD IGM Center on an Illumina NGS HiSeq 4000, with 2x 75 paired-end kits. Illumina HiSeq 4000 SRX18289698 GSM6735363_r1 SRP408310 PRJNA902397 SRS15781434 GSM6735363 GSM6735363 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Livers (> 0.5g) were chopped in cold DMEM (Gibco) and digested with the liver dissociation kit, mouse (Miltenyi Biotec) in DMEM according to the manufacturer's instructions. Samples were dissociated with the gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec) as per the default manufacturers protocol for murine liver digestion. The single cell suspension was filtered through a 70 µM mesh strainer and washed with cold DMEM, resuspended in RBC lysis buffer (eBioscience) then washed twice in autoMACs buffer with BSA (Miltenyi Biotec). CD45+ cells were isolated with CD45 microbeads (Miltenyi Biotec) according to the manufacturer's protocol with the autoMACs separator. The single cell suspension was prepared according to the 10X Genomics single cell sample prep protocol. 6000-8000 cells per sample were loaded onto the 10X Chromium Controller RNA-seq libraries were prepared using Chromium Single Cell 5' v2 Reagent Kits (10X Genomics). libraries were sequenced by UCSD IGM Center on an Illumina NGS HiSeq 4000, with 2x 75 paired-end kits. Illumina HiSeq 4000 SRX18289699 GSM6735364_r1 SRP408310 PRJNA902397 SRS15781435 GSM6735364 GSM6735364 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Livers (> 0.5g) were chopped in cold DMEM (Gibco) and digested with the liver dissociation kit, mouse (Miltenyi Biotec) in DMEM according to the manufacturer's instructions. Samples were dissociated with the gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec) as per the default manufacturers protocol for murine liver digestion. The single cell suspension was filtered through a 70 µM mesh strainer and washed with cold DMEM, resuspended in RBC lysis buffer (eBioscience) then washed twice in autoMACs buffer with BSA (Miltenyi Biotec). CD45+ cells were isolated with CD45 microbeads (Miltenyi Biotec) according to the manufacturer's protocol with the autoMACs separator. The single cell suspension was prepared according to the 10X Genomics single cell sample prep protocol. 6000-8000 cells per sample were loaded onto the 10X Chromium Controller RNA-seq libraries were prepared using Chromium Single Cell 5' v2 Reagent Kits (10X Genomics). libraries were sequenced by UCSD IGM Center on an Illumina NGS HiSeq 4000, with 2x 75 paired-end kits. Illumina HiSeq 4000 SRX18289700 GSM6735365_r1 SRP408310 PRJNA902397 SRS15781436 GSM6735365 GSM6735365 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Livers (> 0.5g) were chopped in cold DMEM (Gibco) and digested with the liver dissociation kit, mouse (Miltenyi Biotec) in DMEM according to the manufacturer's instructions. Samples were dissociated with the gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec) as per the default manufacturers protocol for murine liver digestion. The single cell suspension was filtered through a 70 µM mesh strainer and washed with cold DMEM, resuspended in RBC lysis buffer (eBioscience) then washed twice in autoMACs buffer with BSA (Miltenyi Biotec). CD45+ cells were isolated with CD45 microbeads (Miltenyi Biotec) according to the manufacturer's protocol with the autoMACs separator. The single cell suspension was prepared according to the 10X Genomics single cell sample prep protocol. 6000-8000 cells per sample were loaded onto the 10X Chromium Controller RNA-seq libraries were prepared using Chromium Single Cell 5' v2 Reagent Kits (10X Genomics). libraries were sequenced by UCSD IGM Center on an Illumina NGS HiSeq 4000, with 2x 75 paired-end kits. Illumina HiSeq 4000 SRX18289701 GSM6735366_r1 SRP408310 PRJNA902397 SRS15781437 GSM6735366 GSM6735366 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Livers (> 0.5g) were chopped in cold DMEM (Gibco) and digested with the liver dissociation kit, mouse (Miltenyi Biotec) in DMEM according to the manufacturer's instructions. Samples were dissociated with the gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec) as per the default manufacturers protocol for murine liver digestion. The single cell suspension was filtered through a 70 µM mesh strainer and washed with cold DMEM, resuspended in RBC lysis buffer (eBioscience) then washed twice in autoMACs buffer with BSA (Miltenyi Biotec). CD45+ cells were isolated with CD45 microbeads (Miltenyi Biotec) according to the manufacturer's protocol with the autoMACs separator. The single cell suspension was prepared according to the 10X Genomics single cell sample prep protocol. 6000-8000 cells per sample were loaded onto the 10X Chromium Controller RNA-seq libraries were prepared using Chromium Single Cell 5' v2 Reagent Kits (10X Genomics). libraries were sequenced by UCSD IGM Center on an Illumina NGS HiSeq 4000, with 2x 75 paired-end kits. Illumina HiSeq 4000 SRX18289702 GSM6735367_r1 SRP408310 PRJNA902397 SRS15781438 GSM6735367 GSM6735367 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Livers (> 0.5g) were chopped in cold DMEM (Gibco) and digested with the liver dissociation kit, mouse (Miltenyi Biotec) in DMEM according to the manufacturer's instructions. Samples were dissociated with the gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec) as per the default manufacturers protocol for murine liver digestion. The single cell suspension was filtered through a 70 µM mesh strainer and washed with cold DMEM, resuspended in RBC lysis buffer (eBioscience) then washed twice in autoMACs buffer with BSA (Miltenyi Biotec). CD45+ cells were isolated with CD45 microbeads (Miltenyi Biotec) according to the manufacturer's protocol with the autoMACs separator. The single cell suspension was prepared according to the 10X Genomics single cell sample prep protocol. 6000-8000 cells per sample were loaded onto the 10X Chromium Controller RNA-seq libraries were prepared using Chromium Single Cell 5' v2 Reagent Kits (10X Genomics). libraries were sequenced by UCSD IGM Center on an Illumina NGS HiSeq 4000, with 2x 75 paired-end kits. Illumina HiSeq 4000 SRX18289703 GSM6735368_r1 SRP408310 PRJNA902397 SRS15781439 GSM6735368 GSM6735368 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Livers (> 0.5g) were chopped in cold DMEM (Gibco) and digested with the liver dissociation kit, mouse (Miltenyi Biotec) in DMEM according to the manufacturer's instructions. Samples were dissociated with the gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec) as per the default manufacturers protocol for murine liver digestion. The single cell suspension was filtered through a 70 µM mesh strainer and washed with cold DMEM, resuspended in RBC lysis buffer (eBioscience) then washed twice in autoMACs buffer with BSA (Miltenyi Biotec). CD45+ cells were isolated with CD45 microbeads (Miltenyi Biotec) according to the manufacturer's protocol with the autoMACs separator. The single cell suspension was prepared according to the 10X Genomics single cell sample prep protocol. 6000-8000 cells per sample were loaded onto the 10X Chromium Controller RNA-seq libraries were prepared using Chromium Single Cell 5' v2 Reagent Kits (10X Genomics). libraries were sequenced by UCSD IGM Center on an Illumina NGS HiSeq 4000, with 2x 75 paired-end kits. Illumina HiSeq 4000 SRX18289704 GSM6735369_r1 SRP408310 PRJNA902397 SRS15781440 GSM6735369 GSM6735369 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Livers (> 0.5g) were chopped in cold DMEM (Gibco) and digested with the liver dissociation kit, mouse (Miltenyi Biotec) in DMEM according to the manufacturer's instructions. Samples were dissociated with the gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec) as per the default manufacturers protocol for murine liver digestion. The single cell suspension was filtered through a 70 µM mesh strainer and washed with cold DMEM, resuspended in RBC lysis buffer (eBioscience) then washed twice in autoMACs buffer with BSA (Miltenyi Biotec). CD45+ cells were isolated with CD45 microbeads (Miltenyi Biotec) according to the manufacturer's protocol with the autoMACs separator. The single cell suspension was prepared according to the 10X Genomics single cell sample prep protocol. 6000-8000 cells per sample were loaded onto the 10X Chromium Controller RNA-seq libraries were prepared using Chromium Single Cell 5' v2 Reagent Kits (10X Genomics). libraries were sequenced by UCSD IGM Center on an Illumina NGS HiSeq 4000, with 2x 75 paired-end kits. Illumina HiSeq 4000 SRX18289705 GSM6735370_r1 SRP408310 PRJNA902397 SRS15781441 GSM6735370 GSM6735370 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Livers (> 0.5g) were chopped in cold DMEM (Gibco) and digested with the liver dissociation kit, mouse (Miltenyi Biotec) in DMEM according to the manufacturer's instructions. Samples were dissociated with the gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec) as per the default manufacturers protocol for murine liver digestion. The single cell suspension was filtered through a 70 µM mesh strainer and washed with cold DMEM, resuspended in RBC lysis buffer (eBioscience) then washed twice in autoMACs buffer with BSA (Miltenyi Biotec). CD45+ cells were isolated with CD45 microbeads (Miltenyi Biotec) according to the manufacturer's protocol with the autoMACs separator. The single cell suspension was prepared according to the 10X Genomics single cell sample prep protocol. 6000-8000 cells per sample were loaded onto the 10X Chromium Controller RNA-seq libraries were prepared using Chromium Single Cell 5' v2 Reagent Kits (10X Genomics). libraries were sequenced by UCSD IGM Center on an Illumina NGS HiSeq 4000, with 2x 75 paired-end kits. Illumina HiSeq 4000 SRX18289706 GSM6735371_r1 SRP408310 PRJNA902397 SRS15781442 GSM6735371 GSM6735371 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Livers (> 0.5g) were chopped in cold DMEM (Gibco) and digested with the liver dissociation kit, mouse (Miltenyi Biotec) in DMEM according to the manufacturer's instructions. Samples were dissociated with the gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec) as per the default manufacturers protocol for murine liver digestion. The single cell suspension was filtered through a 70 µM mesh strainer and washed with cold DMEM, resuspended in RBC lysis buffer (eBioscience) then washed twice in autoMACs buffer with BSA (Miltenyi Biotec). CD45+ cells were isolated with CD45 microbeads (Miltenyi Biotec) according to the manufacturer's protocol with the autoMACs separator. The single cell suspension was prepared according to the 10X Genomics single cell sample prep protocol. 6000-8000 cells per sample were loaded onto the 10X Chromium Controller RNA-seq libraries were prepared using Chromium Single Cell 5' v2 Reagent Kits (10X Genomics). libraries were sequenced by UCSD IGM Center on an Illumina NGS HiSeq 4000, with 2x 75 paired-end kits. Illumina HiSeq 4000