GSM6736276: Choroid plexus tissue, Ins2 overexpressed, rep1 [A1]; Mus musculus; RNA-Seq

SRX18293002 GSM6736276_r1 GSM6736276: Choroid plexus tissue, Ins2 overexpressed, rep1 [A1]; Mus musculus; RNA-Seq SRP408415 PRJNA902709 SRS15784415 GSM6736276 GSM6736276 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted by affinity chromatography using PicoPure RNA isolation kit (Thermo Fisher Scientific) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP for directional library construction. Directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification. Illumina NovaSeq 6000 SRX18293003 GSM6736277_r1 GSM6736277: Choroid plexus tissue, Ins2 overexpressed, rep2 [A2]; Mus musculus; RNA-Seq SRP408415 PRJNA902709 SRS15784416 GSM6736277 GSM6736277 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted by affinity chromatography using PicoPure RNA isolation kit (Thermo Fisher Scientific) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP for directional library construction. Directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification. Illumina NovaSeq 6000 SRX18293004 GSM6736278_r1 GSM6736278: Choroid plexus tissue, Ins2 overexpressed, rep3 [A3]; Mus musculus; RNA-Seq SRP408415 PRJNA902709 SRS15784417 GSM6736278 GSM6736278 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted by affinity chromatography using PicoPure RNA isolation kit (Thermo Fisher Scientific) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP for directional library construction. Directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification. Illumina NovaSeq 6000 SRX18293005 GSM6736279_r1 GSM6736279: Choroid plexus tissue, Ins2 overexpressed, rep4 [A4]; Mus musculus; RNA-Seq SRP408415 PRJNA902709 SRS15784418 GSM6736279 GSM6736279 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted by affinity chromatography using PicoPure RNA isolation kit (Thermo Fisher Scientific) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP for directional library construction. Directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification. Illumina NovaSeq 6000 SRX18293006 GSM6736280_r1 GSM6736280: Choroid plexus tissue, Ins2 overexpressed, rep5 [A5]; Mus musculus; RNA-Seq SRP408415 PRJNA902709 SRS15784420 GSM6736280 GSM6736280 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted by affinity chromatography using PicoPure RNA isolation kit (Thermo Fisher Scientific) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP for directional library construction. Directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification. Illumina NovaSeq 6000 SRX18293007 GSM6736281_r1 GSM6736281: Choroid plexus tissue, Ins2 overexpressed, rep6 [A6]; Mus musculus; RNA-Seq SRP408415 PRJNA902709 SRS15784419 GSM6736281 GSM6736281 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted by affinity chromatography using PicoPure RNA isolation kit (Thermo Fisher Scientific) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP for directional library construction. Directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification. Illumina NovaSeq 6000 SRX18293008 GSM6736282_r1 GSM6736282: Choroid plexus tissue, control, rep1 [B1]; Mus musculus; RNA-Seq SRP408415 PRJNA902709 SRS15784421 GSM6736282 GSM6736282 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted by affinity chromatography using PicoPure RNA isolation kit (Thermo Fisher Scientific) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP for directional library construction. Directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification. Illumina NovaSeq 6000 SRX18293009 GSM6736283_r1 GSM6736283: Choroid plexus tissue, control, rep2 [B2]; Mus musculus; RNA-Seq SRP408415 PRJNA902709 SRS15784422 GSM6736283 GSM6736283 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted by affinity chromatography using PicoPure RNA isolation kit (Thermo Fisher Scientific) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP for directional library construction. Directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification. Illumina NovaSeq 6000 SRX18293010 GSM6736284_r1 GSM6736284: Choroid plexus tissue, control, rep3 [B3]; Mus musculus; RNA-Seq SRP408415 PRJNA902709 SRS15784424 GSM6736284 GSM6736284 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted by affinity chromatography using PicoPure RNA isolation kit (Thermo Fisher Scientific) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP for directional library construction. Directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification. Illumina NovaSeq 6000 SRX18293011 GSM6736285_r1 GSM6736285: Choroid plexus tissue, control, rep4 [B4]; Mus musculus; RNA-Seq SRP408415 PRJNA902709 SRS15784423 GSM6736285 GSM6736285 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted by affinity chromatography using PicoPure RNA isolation kit (Thermo Fisher Scientific) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP for directional library construction. Directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification. Illumina NovaSeq 6000 SRX18293012 GSM6736286_r1 GSM6736286: Choroid plexus tissue, control, rep5 [B5]; Mus musculus; RNA-Seq SRP408415 PRJNA902709 SRS15784425 GSM6736286 GSM6736286 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted by affinity chromatography using PicoPure RNA isolation kit (Thermo Fisher Scientific) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP for directional library construction. Directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification. Illumina NovaSeq 6000 SRX18293013 GSM6736287_r1 GSM6736287: Hypothalamus tissue, Ins2 overexpressed in the choroid plexus, rep1 [F1]; Mus musculus; RNA-Seq SRP408415 PRJNA902709 SRS15784426 GSM6736287 GSM6736287 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted by affinity chromatography using PicoPure RNA isolation kit (Thermo Fisher Scientific) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP for directional library construction. Directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification. Illumina NovaSeq 6000 SRX18293014 GSM6736288_r1 GSM6736288: Hypothalamus tissue, Ins2 overexpressed in the choroid plexus, rep2 [F2]; Mus musculus; RNA-Seq SRP408415 PRJNA902709 SRS15784427 GSM6736288 GSM6736288 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted by affinity chromatography using PicoPure RNA isolation kit (Thermo Fisher Scientific) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP for directional library construction. Directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification. Illumina NovaSeq 6000 SRX18293015 GSM6736289_r1 GSM6736289: Hypothalamus tissue, Ins2 overexpressed in the choroid plexus, rep3 [F3]; Mus musculus; RNA-Seq SRP408415 PRJNA902709 SRS15784428 GSM6736289 GSM6736289 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted by affinity chromatography using PicoPure RNA isolation kit (Thermo Fisher Scientific) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP for directional library construction. Directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification. Illumina NovaSeq 6000 SRX18293016 GSM6736290_r1 GSM6736290: Hypothalamus tissue, Ins2 overexpressed in the choroid plexus, rep4 [F5]; Mus musculus; RNA-Seq SRP408415 PRJNA902709 SRS15784429 GSM6736290 GSM6736290 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted by affinity chromatography using PicoPure RNA isolation kit (Thermo Fisher Scientific) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP for directional library construction. Directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification. Illumina NovaSeq 6000 SRX18293017 GSM6736291_r1 GSM6736291: Hypothalamus tissue, Ins2 overexpressed in the choroid plexus, rep5 [F6]; Mus musculus; RNA-Seq SRP408415 PRJNA902709 SRS15784430 GSM6736291 GSM6736291 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted by affinity chromatography using PicoPure RNA isolation kit (Thermo Fisher Scientific) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP for directional library construction. Directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification. Illumina NovaSeq 6000 SRX18293018 GSM6736292_r1 GSM6736292: Hypothalamus tissue, control, rep1 [G1]; Mus musculus; RNA-Seq SRP408415 PRJNA902709 SRS15784431 GSM6736292 GSM6736292 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted by affinity chromatography using PicoPure RNA isolation kit (Thermo Fisher Scientific) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP for directional library construction. Directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification. Illumina NovaSeq 6000 SRX18293019 GSM6736293_r1 GSM6736293: Hypothalamus tissue, control, rep2 [G2]; Mus musculus; RNA-Seq SRP408415 PRJNA902709 SRS15784432 GSM6736293 GSM6736293 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted by affinity chromatography using PicoPure RNA isolation kit (Thermo Fisher Scientific) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP for directional library construction. Directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification. Illumina NovaSeq 6000 SRX18293020 GSM6736294_r1 GSM6736294: Hypothalamus tissue, control, rep3 [G3]; Mus musculus; RNA-Seq SRP408415 PRJNA902709 SRS15784433 GSM6736294 GSM6736294 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted by affinity chromatography using PicoPure RNA isolation kit (Thermo Fisher Scientific) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP for directional library construction. Directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification. Illumina NovaSeq 6000 SRX18293021 GSM6736295_r1 GSM6736295: Hypothalamus tissue, control, rep4 [G4]; Mus musculus; RNA-Seq SRP408415 PRJNA902709 SRS15784434 GSM6736295 GSM6736295 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted by affinity chromatography using PicoPure RNA isolation kit (Thermo Fisher Scientific) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP for directional library construction. Directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification. Illumina NovaSeq 6000 SRX18293022 GSM6736296_r1 GSM6736296: Hypothalamus tissue, control, rep6 [G5]; Mus musculus; RNA-Seq SRP408415 PRJNA902709 SRS15784435 GSM6736296 GSM6736296 RNA-Seq TRANSCRIPTOMIC cDNA RNA was extracted by affinity chromatography using PicoPure RNA isolation kit (Thermo Fisher Scientific) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP for directional library construction. Directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification. Illumina NovaSeq 6000