SRX18298171 GSM6737656_r1 SRP392101 PRJNA869455 SRS15789140 GSM6737656 GSM6737656 OTHER GENOMIC other eSPAN in mESC, mouse B cells and HeLa cells were performed exactly as previously described (Li et al., 2020).GRO-seq (global run-on sequencing) was performed as previous described (Barbieri, E. et al., 2020). GRO-seq libraries were prepared and deep sequencing was performed by Columbia University Genome Center. For CUT&TAG, CUT&RUN and eSPAN libraries, the supernatants were purified with ChIP DNA concentrator columns and library PCR was performed using standard Illumina Nextera Dual Indexing primers. Samples were pooled and sequenced using paired-end sequencing on Illumina NextSeq 500 platforms at Columbia University Genome Center. NextSeq 500 SRX18298173 GSM6737658_r1 SRP392101 PRJNA869455 SRS15789142 GSM6737658 GSM6737658 OTHER GENOMIC other eSPAN in mESC, mouse B cells and HeLa cells were performed exactly as previously described (Li et al., 2020).GRO-seq (global run-on sequencing) was performed as previous described (Barbieri, E. et al., 2020). GRO-seq libraries were prepared and deep sequencing was performed by Columbia University Genome Center. For CUT&TAG, CUT&RUN and eSPAN libraries, the supernatants were purified with ChIP DNA concentrator columns and library PCR was performed using standard Illumina Nextera Dual Indexing primers. Samples were pooled and sequenced using paired-end sequencing on Illumina NextSeq 500 platforms at Columbia University Genome Center. NextSeq 500 SRX18298174 GSM6737659_r1 SRP392101 PRJNA869455 SRS15789143 GSM6737659 GSM6737659 OTHER GENOMIC other eSPAN in mESC, mouse B cells and HeLa cells were performed exactly as previously described (Li et al., 2020).GRO-seq (global run-on sequencing) was performed as previous described (Barbieri, E. et al., 2020). GRO-seq libraries were prepared and deep sequencing was performed by Columbia University Genome Center. For CUT&TAG, CUT&RUN and eSPAN libraries, the supernatants were purified with ChIP DNA concentrator columns and library PCR was performed using standard Illumina Nextera Dual Indexing primers. Samples were pooled and sequenced using paired-end sequencing on Illumina NextSeq 500 platforms at Columbia University Genome Center. NextSeq 500 SRX18298175 GSM6737660_r1 SRP392101 PRJNA869455 SRS15789144 GSM6737660 GSM6737660 OTHER GENOMIC other eSPAN in mESC, mouse B cells and HeLa cells were performed exactly as previously described (Li et al., 2020).GRO-seq (global run-on sequencing) was performed as previous described (Barbieri, E. et al., 2020). GRO-seq libraries were prepared and deep sequencing was performed by Columbia University Genome Center. For CUT&TAG, CUT&RUN and eSPAN libraries, the supernatants were purified with ChIP DNA concentrator columns and library PCR was performed using standard Illumina Nextera Dual Indexing primers. Samples were pooled and sequenced using paired-end sequencing on Illumina NextSeq 500 platforms at Columbia University Genome Center. NextSeq 500 SRX18298176 GSM6737661_r1 SRP392101 PRJNA869455 SRS15789145 GSM6737661 GSM6737661 OTHER GENOMIC other eSPAN in mESC, mouse B cells and HeLa cells were performed exactly as previously described (Li et al., 2020).GRO-seq (global run-on sequencing) was performed as previous described (Barbieri, E. et al., 2020). GRO-seq libraries were prepared and deep sequencing was performed by Columbia University Genome Center. For CUT&TAG, CUT&RUN and eSPAN libraries, the supernatants were purified with ChIP DNA concentrator columns and library PCR was performed using standard Illumina Nextera Dual Indexing primers. Samples were pooled and sequenced using paired-end sequencing on Illumina NextSeq 500 platforms at Columbia University Genome Center. NextSeq 500 SRX18298177 GSM6737662_r1 SRP392101 PRJNA869455 SRS15789146 GSM6737662 GSM6737662 OTHER GENOMIC other eSPAN in mESC, mouse B cells and HeLa cells were performed exactly as previously described (Li et al., 2020).GRO-seq (global run-on sequencing) was performed as previous described (Barbieri, E. et al., 2020). GRO-seq libraries were prepared and deep sequencing was performed by Columbia University Genome Center. For CUT&TAG, CUT&RUN and eSPAN libraries, the supernatants were purified with ChIP DNA concentrator columns and library PCR was performed using standard Illumina Nextera Dual Indexing primers. Samples were pooled and sequenced using paired-end sequencing on Illumina NextSeq 500 platforms at Columbia University Genome Center. NextSeq 500 SRX18298178 GSM6737663_r1 SRP392101 PRJNA869455 SRS15789147 GSM6737663 GSM6737663 OTHER GENOMIC other eSPAN in mESC, mouse B cells and HeLa cells were performed exactly as previously described (Li et al., 2020).GRO-seq (global run-on sequencing) was performed as previous described (Barbieri, E. et al., 2020). GRO-seq libraries were prepared and deep sequencing was performed by Columbia University Genome Center. For CUT&TAG, CUT&RUN and eSPAN libraries, the supernatants were purified with ChIP DNA concentrator columns and library PCR was performed using standard Illumina Nextera Dual Indexing primers. Samples were pooled and sequenced using paired-end sequencing on Illumina NextSeq 500 platforms at Columbia University Genome Center. NextSeq 500 SRX18298179 GSM6737664_r1 SRP392101 PRJNA869455 SRS15789148 GSM6737664 GSM6737664 OTHER GENOMIC other eSPAN in mESC, mouse B cells and HeLa cells were performed exactly as previously described (Li et al., 2020).GRO-seq (global run-on sequencing) was performed as previous described (Barbieri, E. et al., 2020). GRO-seq libraries were prepared and deep sequencing was performed by Columbia University Genome Center. For CUT&TAG, CUT&RUN and eSPAN libraries, the supernatants were purified with ChIP DNA concentrator columns and library PCR was performed using standard Illumina Nextera Dual Indexing primers. Samples were pooled and sequenced using paired-end sequencing on Illumina NextSeq 500 platforms at Columbia University Genome Center. NextSeq 500 SRX18298180 GSM6737665_r1 SRP392101 PRJNA869455 SRS15789149 GSM6737665 GSM6737665 OTHER GENOMIC other eSPAN in mESC, mouse B cells and HeLa cells were performed exactly as previously described (Li et al., 2020).GRO-seq (global run-on sequencing) was performed as previous described (Barbieri, E. et al., 2020). GRO-seq libraries were prepared and deep sequencing was performed by Columbia University Genome Center. For CUT&TAG, CUT&RUN and eSPAN libraries, the supernatants were purified with ChIP DNA concentrator columns and library PCR was performed using standard Illumina Nextera Dual Indexing primers. Samples were pooled and sequenced using paired-end sequencing on Illumina NextSeq 500 platforms at Columbia University Genome Center. NextSeq 500 SRX18298182 GSM6737667_r1 SRP392101 PRJNA869455 SRS15789151 GSM6737667 GSM6737667 OTHER GENOMIC other eSPAN in mESC, mouse B cells and HeLa cells were performed exactly as previously described (Li et al., 2020).GRO-seq (global run-on sequencing) was performed as previous described (Barbieri, E. et al., 2020). GRO-seq libraries were prepared and deep sequencing was performed by Columbia University Genome Center. For CUT&TAG, CUT&RUN and eSPAN libraries, the supernatants were purified with ChIP DNA concentrator columns and library PCR was performed using standard Illumina Nextera Dual Indexing primers. Samples were pooled and sequenced using paired-end sequencing on Illumina NextSeq 500 platforms at Columbia University Genome Center. NextSeq 500 SRX18298183 GSM6737668_r1 SRP392101 PRJNA869455 SRS15789152 GSM6737668 GSM6737668 OTHER GENOMIC other eSPAN in mESC, mouse B cells and HeLa cells were performed exactly as previously described (Li et al., 2020).GRO-seq (global run-on sequencing) was performed as previous described (Barbieri, E. et al., 2020). GRO-seq libraries were prepared and deep sequencing was performed by Columbia University Genome Center. For CUT&TAG, CUT&RUN and eSPAN libraries, the supernatants were purified with ChIP DNA concentrator columns and library PCR was performed using standard Illumina Nextera Dual Indexing primers. Samples were pooled and sequenced using paired-end sequencing on Illumina NextSeq 500 platforms at Columbia University Genome Center. NextSeq 500 SRX18298184 GSM6737669_r1 SRP392101 PRJNA869455 SRS15789153 GSM6737669 GSM6737669 OTHER GENOMIC other eSPAN in mESC, mouse B cells and HeLa cells were performed exactly as previously described (Li et al., 2020).GRO-seq (global run-on sequencing) was performed as previous described (Barbieri, E. et al., 2020). GRO-seq libraries were prepared and deep sequencing was performed by Columbia University Genome Center. For CUT&TAG, CUT&RUN and eSPAN libraries, the supernatants were purified with ChIP DNA concentrator columns and library PCR was performed using standard Illumina Nextera Dual Indexing primers. Samples were pooled and sequenced using paired-end sequencing on Illumina NextSeq 500 platforms at Columbia University Genome Center. NextSeq 500 SRX18298185 GSM6737670_r1 SRP392101 PRJNA869455 SRS15789154 GSM6737670 GSM6737670 OTHER GENOMIC other eSPAN in mESC, mouse B cells and HeLa cells were performed exactly as previously described (Li et al., 2020).GRO-seq (global run-on sequencing) was performed as previous described (Barbieri, E. et al., 2020). GRO-seq libraries were prepared and deep sequencing was performed by Columbia University Genome Center. For CUT&TAG, CUT&RUN and eSPAN libraries, the supernatants were purified with ChIP DNA concentrator columns and library PCR was performed using standard Illumina Nextera Dual Indexing primers. Samples were pooled and sequenced using paired-end sequencing on Illumina NextSeq 500 platforms at Columbia University Genome Center. NextSeq 500 SRX18298186 GSM6737671_r1 SRP392101 PRJNA869455 SRS15789155 GSM6737671 GSM6737671 OTHER GENOMIC other eSPAN in mESC, mouse B cells and HeLa cells were performed exactly as previously described (Li et al., 2020).GRO-seq (global run-on sequencing) was performed as previous described (Barbieri, E. et al., 2020). GRO-seq libraries were prepared and deep sequencing was performed by Columbia University Genome Center. For CUT&TAG, CUT&RUN and eSPAN libraries, the supernatants were purified with ChIP DNA concentrator columns and library PCR was performed using standard Illumina Nextera Dual Indexing primers. Samples were pooled and sequenced using paired-end sequencing on Illumina NextSeq 500 platforms at Columbia University Genome Center. NextSeq 500 SRX18298187 GSM6737672_r1 SRP392101 PRJNA869455 SRS15789156 GSM6737672 GSM6737672 OTHER GENOMIC other eSPAN in mESC, mouse B cells and HeLa cells were performed exactly as previously described (Li et al., 2020).GRO-seq (global run-on sequencing) was performed as previous described (Barbieri, E. et al., 2020). GRO-seq libraries were prepared and deep sequencing was performed by Columbia University Genome Center. For CUT&TAG, CUT&RUN and eSPAN libraries, the supernatants were purified with ChIP DNA concentrator columns and library PCR was performed using standard Illumina Nextera Dual Indexing primers. Samples were pooled and sequenced using paired-end sequencing on Illumina NextSeq 500 platforms at Columbia University Genome Center. NextSeq 500 SRX18298189 GSM6737674_r1 SRP392101 PRJNA869455 SRS15789158 GSM6737674 GSM6737674 OTHER GENOMIC other eSPAN in mESC, mouse B cells and HeLa cells were performed exactly as previously described (Li et al., 2020).GRO-seq (global run-on sequencing) was performed as previous described (Barbieri, E. et al., 2020). GRO-seq libraries were prepared and deep sequencing was performed by Columbia University Genome Center. For CUT&TAG, CUT&RUN and eSPAN libraries, the supernatants were purified with ChIP DNA concentrator columns and library PCR was performed using standard Illumina Nextera Dual Indexing primers. Samples were pooled and sequenced using paired-end sequencing on Illumina NextSeq 500 platforms at Columbia University Genome Center. NextSeq 500 SRX18298190 GSM6737675_r1 SRP392101 PRJNA869455 SRS15789159 GSM6737675 GSM6737675 OTHER GENOMIC other eSPAN in mESC, mouse B cells and HeLa cells were performed exactly as previously described (Li et al., 2020).GRO-seq (global run-on sequencing) was performed as previous described (Barbieri, E. et al., 2020). GRO-seq libraries were prepared and deep sequencing was performed by Columbia University Genome Center. For CUT&TAG, CUT&RUN and eSPAN libraries, the supernatants were purified with ChIP DNA concentrator columns and library PCR was performed using standard Illumina Nextera Dual Indexing primers. Samples were pooled and sequenced using paired-end sequencing on Illumina NextSeq 500 platforms at Columbia University Genome Center. NextSeq 500