SRP408546 PRJNA902961 2015/2018-GCEF-16S-18S-ITS The study site was the Global Change Experimental Facility (GCEF) research station in Central Germany. Microbial community composition was determined by sequencing 16S/18S rRNA gene and ITS1 amplicons using soil DNA samples from intensive grassland treatment in 2015 and 2018 summers.