SRX18311682 DMSO11 SRP408642 bp0 PCR on the gDNA samples to amplify the sgRNA guide sequence region and to append the Illumina (HiSeq) compatible adapters and barcodes. A two-step PCR was performed: First, the amount of input gDNA for each sample was calculated in order to achieve 500X library coverage. For each replicate sample, 22 separate 100 l PCR reactions with 10 g of input genomic DNA in each reaction with Herculase II Fusion DNA Polymerase (Agilent, Cat#600677) were completed in an ABI Veriti Thermal Cycler (Applied Biosystems). Amplicons from the first PCRs were then pooled together and mixed thoroughly to be used as the template for the second PCR reaction. The second PCR was designed to attach standard Illumina adaptors and unique barcodes to the amplicons from the first PCR to allow for HiSeq reactions and demultiplexing of the reads, respectively. The second PCR was conducted on 5 l of the first PCR amplicon as the template, in 100 l reactions with a total of 22 reactions. Primers for the second PCR (Table S2) were synthesized as IDT DNA Ultramer Oligonucleotides (Integrated DNA Technologies) and also included a staggered region with variable lengths of sequence to enhance the library complexity. SRS15800369 DMSO11 DMSO11 WGS GENOMIC PCR Illumina HiSeq 2500 SRX18311683 DMSO21 SRP408642 bp0 PCR on the gDNA samples to amplify the sgRNA guide sequence region and to append the Illumina (HiSeq) compatible adapters and barcodes. A two-step PCR was performed: First, the amount of input gDNA for each sample was calculated in order to achieve 500X library coverage. For each replicate sample, 22 separate 100 l PCR reactions with 10 g of input genomic DNA in each reaction with Herculase II Fusion DNA Polymerase (Agilent, Cat#600677) were completed in an ABI Veriti Thermal Cycler (Applied Biosystems). Amplicons from the first PCRs were then pooled together and mixed thoroughly to be used as the template for the second PCR reaction. The second PCR was designed to attach standard Illumina adaptors and unique barcodes to the amplicons from the first PCR to allow for HiSeq reactions and demultiplexing of the reads, respectively. The second PCR was conducted on 5 l of the first PCR amplicon as the template, in 100 l reactions with a total of 22 reactions. Primers for the second PCR (Table S2) were synthesized as IDT DNA Ultramer Oligonucleotides (Integrated DNA Technologies) and also included a staggered region with variable lengths of sequence to enhance the library complexity. SRS15800370 DMSO21 DMSO21 WGS GENOMIC PCR Illumina HiSeq 2500 SRX18311684 Day011 SRP408642 bp0 PCR on the gDNA samples to amplify the sgRNA guide sequence region and to append the Illumina (HiSeq) compatible adapters and barcodes. A two-step PCR was performed: First, the amount of input gDNA for each sample was calculated in order to achieve 500X library coverage. For each replicate sample, 22 separate 100 l PCR reactions with 10 g of input genomic DNA in each reaction with Herculase II Fusion DNA Polymerase (Agilent, Cat#600677) were completed in an ABI Veriti Thermal Cycler (Applied Biosystems). Amplicons from the first PCRs were then pooled together and mixed thoroughly to be used as the template for the second PCR reaction. The second PCR was designed to attach standard Illumina adaptors and unique barcodes to the amplicons from the first PCR to allow for HiSeq reactions and demultiplexing of the reads, respectively. The second PCR was conducted on 5 l of the first PCR amplicon as the template, in 100 l reactions with a total of 22 reactions. Primers for the second PCR (Table S2) were synthesized as IDT DNA Ultramer Oligonucleotides (Integrated DNA Technologies) and also included a staggered region with variable lengths of sequence to enhance the library complexity. SRS15800371 Day011 Day011 WGS GENOMIC PCR Illumina HiSeq 2500 SRX18311685 Day021 SRP408642 bp0 PCR on the gDNA samples to amplify the sgRNA guide sequence region and to append the Illumina (HiSeq) compatible adapters and barcodes. A two-step PCR was performed: First, the amount of input gDNA for each sample was calculated in order to achieve 500X library coverage. For each replicate sample, 22 separate 100 l PCR reactions with 10 g of input genomic DNA in each reaction with Herculase II Fusion DNA Polymerase (Agilent, Cat#600677) were completed in an ABI Veriti Thermal Cycler (Applied Biosystems). Amplicons from the first PCRs were then pooled together and mixed thoroughly to be used as the template for the second PCR reaction. The second PCR was designed to attach standard Illumina adaptors and unique barcodes to the amplicons from the first PCR to allow for HiSeq reactions and demultiplexing of the reads, respectively. The second PCR was conducted on 5 l of the first PCR amplicon as the template, in 100 l reactions with a total of 22 reactions. Primers for the second PCR (Table S2) were synthesized as IDT DNA Ultramer Oligonucleotides (Integrated DNA Technologies) and also included a staggered region with variable lengths of sequence to enhance the library complexity. SRS15800372 Day021 Day021 WGS GENOMIC PCR Illumina HiSeq 2500 SRX18311686 DMSO12 SRP408642 bp0 PCR on the gDNA samples to amplify the sgRNA guide sequence region and to append the Illumina (HiSeq) compatible adapters and barcodes. A two-step PCR was performed: First, the amount of input gDNA for each sample was calculated in order to achieve 500X library coverage. For each replicate sample, 22 separate 100 l PCR reactions with 10 g of input genomic DNA in each reaction with Herculase II Fusion DNA Polymerase (Agilent, Cat#600677) were completed in an ABI Veriti Thermal Cycler (Applied Biosystems). Amplicons from the first PCRs were then pooled together and mixed thoroughly to be used as the template for the second PCR reaction. The second PCR was designed to attach standard Illumina adaptors and unique barcodes to the amplicons from the first PCR to allow for HiSeq reactions and demultiplexing of the reads, respectively. The second PCR was conducted on 5 l of the first PCR amplicon as the template, in 100 l reactions with a total of 22 reactions. Primers for the second PCR (Table S2) were synthesized as IDT DNA Ultramer Oligonucleotides (Integrated DNA Technologies) and also included a staggered region with variable lengths of sequence to enhance the library complexity. SRS15800374 DMSO12 DMSO12 WGS GENOMIC PCR Illumina HiSeq 2500 SRX18311687 DMSO22 SRP408642 bp0 PCR on the gDNA samples to amplify the sgRNA guide sequence region and to append the Illumina (HiSeq) compatible adapters and barcodes. A two-step PCR was performed: First, the amount of input gDNA for each sample was calculated in order to achieve 500X library coverage. For each replicate sample, 22 separate 100 l PCR reactions with 10 g of input genomic DNA in each reaction with Herculase II Fusion DNA Polymerase (Agilent, Cat#600677) were completed in an ABI Veriti Thermal Cycler (Applied Biosystems). Amplicons from the first PCRs were then pooled together and mixed thoroughly to be used as the template for the second PCR reaction. The second PCR was designed to attach standard Illumina adaptors and unique barcodes to the amplicons from the first PCR to allow for HiSeq reactions and demultiplexing of the reads, respectively. The second PCR was conducted on 5 l of the first PCR amplicon as the template, in 100 l reactions with a total of 22 reactions. Primers for the second PCR (Table S2) were synthesized as IDT DNA Ultramer Oligonucleotides (Integrated DNA Technologies) and also included a staggered region with variable lengths of sequence to enhance the library complexity. SRS15800373 DMSO22 DMSO22 WGS GENOMIC PCR Illumina HiSeq 2500 SRX18311688 AP30012 SRP408642 bp0 PCR on the gDNA samples to amplify the sgRNA guide sequence region and to append the Illumina (HiSeq) compatible adapters and barcodes. A two-step PCR was performed: First, the amount of input gDNA for each sample was calculated in order to achieve 500X library coverage. For each replicate sample, 22 separate 100 l PCR reactions with 10 g of input genomic DNA in each reaction with Herculase II Fusion DNA Polymerase (Agilent, Cat#600677) were completed in an ABI Veriti Thermal Cycler (Applied Biosystems). Amplicons from the first PCRs were then pooled together and mixed thoroughly to be used as the template for the second PCR reaction. The second PCR was designed to attach standard Illumina adaptors and unique barcodes to the amplicons from the first PCR to allow for HiSeq reactions and demultiplexing of the reads, respectively. The second PCR was conducted on 5 l of the first PCR amplicon as the template, in 100 l reactions with a total of 22 reactions. Primers for the second PCR (Table S2) were synthesized as IDT DNA Ultramer Oligonucleotides (Integrated DNA Technologies) and also included a staggered region with variable lengths of sequence to enhance the library complexity. SRS15800375 AP30012 AP30012 WGS GENOMIC PCR Illumina HiSeq 2500 SRX18311689 AP30022 SRP408642 bp0 PCR on the gDNA samples to amplify the sgRNA guide sequence region and to append the Illumina (HiSeq) compatible adapters and barcodes. A two-step PCR was performed: First, the amount of input gDNA for each sample was calculated in order to achieve 500X library coverage. For each replicate sample, 22 separate 100 l PCR reactions with 10 g of input genomic DNA in each reaction with Herculase II Fusion DNA Polymerase (Agilent, Cat#600677) were completed in an ABI Veriti Thermal Cycler (Applied Biosystems). Amplicons from the first PCRs were then pooled together and mixed thoroughly to be used as the template for the second PCR reaction. The second PCR was designed to attach standard Illumina adaptors and unique barcodes to the amplicons from the first PCR to allow for HiSeq reactions and demultiplexing of the reads, respectively. The second PCR was conducted on 5 l of the first PCR amplicon as the template, in 100 l reactions with a total of 22 reactions. Primers for the second PCR (Table S2) were synthesized as IDT DNA Ultramer Oligonucleotides (Integrated DNA Technologies) and also included a staggered region with variable lengths of sequence to enhance the library complexity. SRS15800376 AP30022 AP30022 WGS GENOMIC PCR Illumina HiSeq 2500 SRX18311690 AP75012 SRP408642 bp0 PCR on the gDNA samples to amplify the sgRNA guide sequence region and to append the Illumina (HiSeq) compatible adapters and barcodes. A two-step PCR was performed: First, the amount of input gDNA for each sample was calculated in order to achieve 500X library coverage. For each replicate sample, 22 separate 100 l PCR reactions with 10 g of input genomic DNA in each reaction with Herculase II Fusion DNA Polymerase (Agilent, Cat#600677) were completed in an ABI Veriti Thermal Cycler (Applied Biosystems). Amplicons from the first PCRs were then pooled together and mixed thoroughly to be used as the template for the second PCR reaction. The second PCR was designed to attach standard Illumina adaptors and unique barcodes to the amplicons from the first PCR to allow for HiSeq reactions and demultiplexing of the reads, respectively. The second PCR was conducted on 5 l of the first PCR amplicon as the template, in 100 l reactions with a total of 22 reactions. Primers for the second PCR (Table S2) were synthesized as IDT DNA Ultramer Oligonucleotides (Integrated DNA Technologies) and also included a staggered region with variable lengths of sequence to enhance the library complexity. SRS15800377 AP75012 AP75012 WGS GENOMIC PCR Illumina HiSeq 2500 SRX18311691 AP75022 SRP408642 bp0 PCR on the gDNA samples to amplify the sgRNA guide sequence region and to append the Illumina (HiSeq) compatible adapters and barcodes. A two-step PCR was performed: First, the amount of input gDNA for each sample was calculated in order to achieve 500X library coverage. For each replicate sample, 22 separate 100 l PCR reactions with 10 g of input genomic DNA in each reaction with Herculase II Fusion DNA Polymerase (Agilent, Cat#600677) were completed in an ABI Veriti Thermal Cycler (Applied Biosystems). Amplicons from the first PCRs were then pooled together and mixed thoroughly to be used as the template for the second PCR reaction. The second PCR was designed to attach standard Illumina adaptors and unique barcodes to the amplicons from the first PCR to allow for HiSeq reactions and demultiplexing of the reads, respectively. The second PCR was conducted on 5 l of the first PCR amplicon as the template, in 100 l reactions with a total of 22 reactions. Primers for the second PCR (Table S2) were synthesized as IDT DNA Ultramer Oligonucleotides (Integrated DNA Technologies) and also included a staggered region with variable lengths of sequence to enhance the library complexity. SRS15800378 AP75022 AP75022 WGS GENOMIC PCR Illumina HiSeq 2500 SRX18311692 LDK30012 SRP408642 bp0 PCR on the gDNA samples to amplify the sgRNA guide sequence region and to append the Illumina (HiSeq) compatible adapters and barcodes. A two-step PCR was performed: First, the amount of input gDNA for each sample was calculated in order to achieve 500X library coverage. For each replicate sample, 22 separate 100 l PCR reactions with 10 g of input genomic DNA in each reaction with Herculase II Fusion DNA Polymerase (Agilent, Cat#600677) were completed in an ABI Veriti Thermal Cycler (Applied Biosystems). Amplicons from the first PCRs were then pooled together and mixed thoroughly to be used as the template for the second PCR reaction. The second PCR was designed to attach standard Illumina adaptors and unique barcodes to the amplicons from the first PCR to allow for HiSeq reactions and demultiplexing of the reads, respectively. The second PCR was conducted on 5 l of the first PCR amplicon as the template, in 100 l reactions with a total of 22 reactions. Primers for the second PCR (Table S2) were synthesized as IDT DNA Ultramer Oligonucleotides (Integrated DNA Technologies) and also included a staggered region with variable lengths of sequence to enhance the library complexity. SRS15800379 LDK30012 LDK30012 WGS GENOMIC PCR Illumina HiSeq 2500 SRX18311693 LDK30022 SRP408642 bp0 PCR on the gDNA samples to amplify the sgRNA guide sequence region and to append the Illumina (HiSeq) compatible adapters and barcodes. A two-step PCR was performed: First, the amount of input gDNA for each sample was calculated in order to achieve 500X library coverage. For each replicate sample, 22 separate 100 l PCR reactions with 10 g of input genomic DNA in each reaction with Herculase II Fusion DNA Polymerase (Agilent, Cat#600677) were completed in an ABI Veriti Thermal Cycler (Applied Biosystems). Amplicons from the first PCRs were then pooled together and mixed thoroughly to be used as the template for the second PCR reaction. The second PCR was designed to attach standard Illumina adaptors and unique barcodes to the amplicons from the first PCR to allow for HiSeq reactions and demultiplexing of the reads, respectively. The second PCR was conducted on 5 l of the first PCR amplicon as the template, in 100 l reactions with a total of 22 reactions. Primers for the second PCR (Table S2) were synthesized as IDT DNA Ultramer Oligonucleotides (Integrated DNA Technologies) and also included a staggered region with variable lengths of sequence to enhance the library complexity. SRS15800380 LDK30022 LDK30022 WGS GENOMIC PCR Illumina HiSeq 2500 SRX18311694 AP30011 SRP408642 bp0 PCR on the gDNA samples to amplify the sgRNA guide sequence region and to append the Illumina (HiSeq) compatible adapters and barcodes. A two-step PCR was performed: First, the amount of input gDNA for each sample was calculated in order to achieve 500X library coverage. For each replicate sample, 22 separate 100 l PCR reactions with 10 g of input genomic DNA in each reaction with Herculase II Fusion DNA Polymerase (Agilent, Cat#600677) were completed in an ABI Veriti Thermal Cycler (Applied Biosystems). Amplicons from the first PCRs were then pooled together and mixed thoroughly to be used as the template for the second PCR reaction. The second PCR was designed to attach standard Illumina adaptors and unique barcodes to the amplicons from the first PCR to allow for HiSeq reactions and demultiplexing of the reads, respectively. The second PCR was conducted on 5 l of the first PCR amplicon as the template, in 100 l reactions with a total of 22 reactions. Primers for the second PCR (Table S2) were synthesized as IDT DNA Ultramer Oligonucleotides (Integrated DNA Technologies) and also included a staggered region with variable lengths of sequence to enhance the library complexity. SRS15800381 AP30011 AP30011 WGS GENOMIC PCR Illumina HiSeq 2500 SRX18311695 LDK75012 SRP408642 bp0 PCR on the gDNA samples to amplify the sgRNA guide sequence region and to append the Illumina (HiSeq) compatible adapters and barcodes. A two-step PCR was performed: First, the amount of input gDNA for each sample was calculated in order to achieve 500X library coverage. For each replicate sample, 22 separate 100 l PCR reactions with 10 g of input genomic DNA in each reaction with Herculase II Fusion DNA Polymerase (Agilent, Cat#600677) were completed in an ABI Veriti Thermal Cycler (Applied Biosystems). Amplicons from the first PCRs were then pooled together and mixed thoroughly to be used as the template for the second PCR reaction. The second PCR was designed to attach standard Illumina adaptors and unique barcodes to the amplicons from the first PCR to allow for HiSeq reactions and demultiplexing of the reads, respectively. The second PCR was conducted on 5 l of the first PCR amplicon as the template, in 100 l reactions with a total of 22 reactions. Primers for the second PCR (Table S2) were synthesized as IDT DNA Ultramer Oligonucleotides (Integrated DNA Technologies) and also included a staggered region with variable lengths of sequence to enhance the library complexity. SRS15800382 LDK75012 LDK75012 WGS GENOMIC PCR Illumina HiSeq 2500 SRX18311696 LDK75022 SRP408642 bp0 PCR on the gDNA samples to amplify the sgRNA guide sequence region and to append the Illumina (HiSeq) compatible adapters and barcodes. A two-step PCR was performed: First, the amount of input gDNA for each sample was calculated in order to achieve 500X library coverage. For each replicate sample, 22 separate 100 l PCR reactions with 10 g of input genomic DNA in each reaction with Herculase II Fusion DNA Polymerase (Agilent, Cat#600677) were completed in an ABI Veriti Thermal Cycler (Applied Biosystems). Amplicons from the first PCRs were then pooled together and mixed thoroughly to be used as the template for the second PCR reaction. The second PCR was designed to attach standard Illumina adaptors and unique barcodes to the amplicons from the first PCR to allow for HiSeq reactions and demultiplexing of the reads, respectively. The second PCR was conducted on 5 l of the first PCR amplicon as the template, in 100 l reactions with a total of 22 reactions. Primers for the second PCR (Table S2) were synthesized as IDT DNA Ultramer Oligonucleotides (Integrated DNA Technologies) and also included a staggered region with variable lengths of sequence to enhance the library complexity. SRS15800383 LDK75022 LDK75022 WGS GENOMIC PCR Illumina HiSeq 2500 SRX18311697 Day012 SRP408642 bp0 PCR on the gDNA samples to amplify the sgRNA guide sequence region and to append the Illumina (HiSeq) compatible adapters and barcodes. A two-step PCR was performed: First, the amount of input gDNA for each sample was calculated in order to achieve 500X library coverage. For each replicate sample, 22 separate 100 l PCR reactions with 10 g of input genomic DNA in each reaction with Herculase II Fusion DNA Polymerase (Agilent, Cat#600677) were completed in an ABI Veriti Thermal Cycler (Applied Biosystems). Amplicons from the first PCRs were then pooled together and mixed thoroughly to be used as the template for the second PCR reaction. The second PCR was designed to attach standard Illumina adaptors and unique barcodes to the amplicons from the first PCR to allow for HiSeq reactions and demultiplexing of the reads, respectively. The second PCR was conducted on 5 l of the first PCR amplicon as the template, in 100 l reactions with a total of 22 reactions. Primers for the second PCR (Table S2) were synthesized as IDT DNA Ultramer Oligonucleotides (Integrated DNA Technologies) and also included a staggered region with variable lengths of sequence to enhance the library complexity. SRS15800384 Day012 Day012 WGS GENOMIC PCR Illumina HiSeq 2500 SRX18311698 Day022 SRP408642 bp0 PCR on the gDNA samples to amplify the sgRNA guide sequence region and to append the Illumina (HiSeq) compatible adapters and barcodes. A two-step PCR was performed: First, the amount of input gDNA for each sample was calculated in order to achieve 500X library coverage. For each replicate sample, 22 separate 100 l PCR reactions with 10 g of input genomic DNA in each reaction with Herculase II Fusion DNA Polymerase (Agilent, Cat#600677) were completed in an ABI Veriti Thermal Cycler (Applied Biosystems). Amplicons from the first PCRs were then pooled together and mixed thoroughly to be used as the template for the second PCR reaction. The second PCR was designed to attach standard Illumina adaptors and unique barcodes to the amplicons from the first PCR to allow for HiSeq reactions and demultiplexing of the reads, respectively. The second PCR was conducted on 5 l of the first PCR amplicon as the template, in 100 l reactions with a total of 22 reactions. Primers for the second PCR (Table S2) were synthesized as IDT DNA Ultramer Oligonucleotides (Integrated DNA Technologies) and also included a staggered region with variable lengths of sequence to enhance the library complexity. SRS15800386 Day022 Day022 WGS GENOMIC PCR Illumina HiSeq 2500 SRX18311699 AP30021 SRP408642 bp0 PCR on the gDNA samples to amplify the sgRNA guide sequence region and to append the Illumina (HiSeq) compatible adapters and barcodes. A two-step PCR was performed: First, the amount of input gDNA for each sample was calculated in order to achieve 500X library coverage. For each replicate sample, 22 separate 100 l PCR reactions with 10 g of input genomic DNA in each reaction with Herculase II Fusion DNA Polymerase (Agilent, Cat#600677) were completed in an ABI Veriti Thermal Cycler (Applied Biosystems). Amplicons from the first PCRs were then pooled together and mixed thoroughly to be used as the template for the second PCR reaction. The second PCR was designed to attach standard Illumina adaptors and unique barcodes to the amplicons from the first PCR to allow for HiSeq reactions and demultiplexing of the reads, respectively. The second PCR was conducted on 5 l of the first PCR amplicon as the template, in 100 l reactions with a total of 22 reactions. Primers for the second PCR (Table S2) were synthesized as IDT DNA Ultramer Oligonucleotides (Integrated DNA Technologies) and also included a staggered region with variable lengths of sequence to enhance the library complexity. SRS15800385 AP30021 AP30021 WGS GENOMIC PCR Illumina HiSeq 2500 SRX18311700 AP75011 SRP408642 bp0 PCR on the gDNA samples to amplify the sgRNA guide sequence region and to append the Illumina (HiSeq) compatible adapters and barcodes. A two-step PCR was performed: First, the amount of input gDNA for each sample was calculated in order to achieve 500X library coverage. For each replicate sample, 22 separate 100 l PCR reactions with 10 g of input genomic DNA in each reaction with Herculase II Fusion DNA Polymerase (Agilent, Cat#600677) were completed in an ABI Veriti Thermal Cycler (Applied Biosystems). Amplicons from the first PCRs were then pooled together and mixed thoroughly to be used as the template for the second PCR reaction. The second PCR was designed to attach standard Illumina adaptors and unique barcodes to the amplicons from the first PCR to allow for HiSeq reactions and demultiplexing of the reads, respectively. The second PCR was conducted on 5 l of the first PCR amplicon as the template, in 100 l reactions with a total of 22 reactions. Primers for the second PCR (Table S2) were synthesized as IDT DNA Ultramer Oligonucleotides (Integrated DNA Technologies) and also included a staggered region with variable lengths of sequence to enhance the library complexity. SRS15800387 AP75011 AP75011 WGS GENOMIC PCR Illumina HiSeq 2500 SRX18311701 AP75021 SRP408642 bp0 PCR on the gDNA samples to amplify the sgRNA guide sequence region and to append the Illumina (HiSeq) compatible adapters and barcodes. A two-step PCR was performed: First, the amount of input gDNA for each sample was calculated in order to achieve 500X library coverage. For each replicate sample, 22 separate 100 l PCR reactions with 10 g of input genomic DNA in each reaction with Herculase II Fusion DNA Polymerase (Agilent, Cat#600677) were completed in an ABI Veriti Thermal Cycler (Applied Biosystems). Amplicons from the first PCRs were then pooled together and mixed thoroughly to be used as the template for the second PCR reaction. The second PCR was designed to attach standard Illumina adaptors and unique barcodes to the amplicons from the first PCR to allow for HiSeq reactions and demultiplexing of the reads, respectively. The second PCR was conducted on 5 l of the first PCR amplicon as the template, in 100 l reactions with a total of 22 reactions. Primers for the second PCR (Table S2) were synthesized as IDT DNA Ultramer Oligonucleotides (Integrated DNA Technologies) and also included a staggered region with variable lengths of sequence to enhance the library complexity. SRS15800388 AP75021 AP75021 WGS GENOMIC PCR Illumina HiSeq 2500 SRX18311702 LDK30011 SRP408642 bp0 PCR on the gDNA samples to amplify the sgRNA guide sequence region and to append the Illumina (HiSeq) compatible adapters and barcodes. A two-step PCR was performed: First, the amount of input gDNA for each sample was calculated in order to achieve 500X library coverage. For each replicate sample, 22 separate 100 l PCR reactions with 10 g of input genomic DNA in each reaction with Herculase II Fusion DNA Polymerase (Agilent, Cat#600677) were completed in an ABI Veriti Thermal Cycler (Applied Biosystems). Amplicons from the first PCRs were then pooled together and mixed thoroughly to be used as the template for the second PCR reaction. The second PCR was designed to attach standard Illumina adaptors and unique barcodes to the amplicons from the first PCR to allow for HiSeq reactions and demultiplexing of the reads, respectively. The second PCR was conducted on 5 l of the first PCR amplicon as the template, in 100 l reactions with a total of 22 reactions. Primers for the second PCR (Table S2) were synthesized as IDT DNA Ultramer Oligonucleotides (Integrated DNA Technologies) and also included a staggered region with variable lengths of sequence to enhance the library complexity. SRS15800389 LDK30011 LDK30011 WGS GENOMIC PCR Illumina HiSeq 2500 SRX18311703 LDK30021 SRP408642 bp0 PCR on the gDNA samples to amplify the sgRNA guide sequence region and to append the Illumina (HiSeq) compatible adapters and barcodes. A two-step PCR was performed: First, the amount of input gDNA for each sample was calculated in order to achieve 500X library coverage. For each replicate sample, 22 separate 100 l PCR reactions with 10 g of input genomic DNA in each reaction with Herculase II Fusion DNA Polymerase (Agilent, Cat#600677) were completed in an ABI Veriti Thermal Cycler (Applied Biosystems). Amplicons from the first PCRs were then pooled together and mixed thoroughly to be used as the template for the second PCR reaction. The second PCR was designed to attach standard Illumina adaptors and unique barcodes to the amplicons from the first PCR to allow for HiSeq reactions and demultiplexing of the reads, respectively. The second PCR was conducted on 5 l of the first PCR amplicon as the template, in 100 l reactions with a total of 22 reactions. Primers for the second PCR (Table S2) were synthesized as IDT DNA Ultramer Oligonucleotides (Integrated DNA Technologies) and also included a staggered region with variable lengths of sequence to enhance the library complexity. SRS15800390 LDK30021 LDK30021 WGS GENOMIC PCR Illumina HiSeq 2500 SRX18311704 LDK75011 SRP408642 bp0 PCR on the gDNA samples to amplify the sgRNA guide sequence region and to append the Illumina (HiSeq) compatible adapters and barcodes. A two-step PCR was performed: First, the amount of input gDNA for each sample was calculated in order to achieve 500X library coverage. For each replicate sample, 22 separate 100 l PCR reactions with 10 g of input genomic DNA in each reaction with Herculase II Fusion DNA Polymerase (Agilent, Cat#600677) were completed in an ABI Veriti Thermal Cycler (Applied Biosystems). Amplicons from the first PCRs were then pooled together and mixed thoroughly to be used as the template for the second PCR reaction. The second PCR was designed to attach standard Illumina adaptors and unique barcodes to the amplicons from the first PCR to allow for HiSeq reactions and demultiplexing of the reads, respectively. The second PCR was conducted on 5 l of the first PCR amplicon as the template, in 100 l reactions with a total of 22 reactions. Primers for the second PCR (Table S2) were synthesized as IDT DNA Ultramer Oligonucleotides (Integrated DNA Technologies) and also included a staggered region with variable lengths of sequence to enhance the library complexity. SRS15800391 LDK75011 LDK75011 WGS GENOMIC PCR Illumina HiSeq 2500 SRX18311705 LDK75021 SRP408642 bp0 PCR on the gDNA samples to amplify the sgRNA guide sequence region and to append the Illumina (HiSeq) compatible adapters and barcodes. A two-step PCR was performed: First, the amount of input gDNA for each sample was calculated in order to achieve 500X library coverage. For each replicate sample, 22 separate 100 l PCR reactions with 10 g of input genomic DNA in each reaction with Herculase II Fusion DNA Polymerase (Agilent, Cat#600677) were completed in an ABI Veriti Thermal Cycler (Applied Biosystems). Amplicons from the first PCRs were then pooled together and mixed thoroughly to be used as the template for the second PCR reaction. The second PCR was designed to attach standard Illumina adaptors and unique barcodes to the amplicons from the first PCR to allow for HiSeq reactions and demultiplexing of the reads, respectively. The second PCR was conducted on 5 l of the first PCR amplicon as the template, in 100 l reactions with a total of 22 reactions. Primers for the second PCR (Table S2) were synthesized as IDT DNA Ultramer Oligonucleotides (Integrated DNA Technologies) and also included a staggered region with variable lengths of sequence to enhance the library complexity. SRS15800392 LDK75021 LDK75021 WGS GENOMIC PCR Illumina HiSeq 2500