<?xml version="1.0" encoding="UTF-8"?>
<STUDY_SET xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
  <STUDY center_name="GEO" alias="GSE218490" accession="SRP409023">
    <IDENTIFIERS>
      <PRIMARY_ID>SRP409023</PRIMARY_ID>
      <EXTERNAL_ID namespace="BioProject" label="primary">PRJNA903926</EXTERNAL_ID>
      <EXTERNAL_ID namespace="GEO">GSE218490</EXTERNAL_ID>
    </IDENTIFIERS>
    <DESCRIPTOR>
      <STUDY_TITLE>Impact of heavy metals on cold acclimation of Salix viminalis roots</STUDY_TITLE>
      <STUDY_TYPE existing_study_type="Transcriptome Analysis"/>
      <STUDY_ABSTRACT>Frost is a major abiotic stress limiting plant growth and development. Climate change models predict an increase in the magnitude and frequency of late-frost events, which, together with an observed loss of soil insulation, will significantly damage roots. To withstand freezing stress, plants have evolved an adaptative process known as cold acclimation. While this process is well documented, it is known that the plant response to multiple stresses is unique and cannot be deduced from the response to each stress taken separately. Here, we investigate the impact of long-term metal exposure on the cold acclimation of Salix viminalis roots. To do so, we used physiological, transcriptomic and proteomic approaches. We found that while metal exposure significantly affected plants morphology and physiology, it did not impede cold acclimation. The impact of the simultaneous exposure to metals and cold acclimation on the transcriptome was unique, while at the proteomic level, the cold acclimation component seemed to be dominant. Further analysis revealed that metals strongly and negatively impacted the cellular antioxidant system. While this should have led to a loss of frost tolerance, it was not observed. A group of proteins was identified that could have played a role in compensating the impediment of the antioxidative system in metal-exposed roots. Overall design: Cuttings of 10 cm of one individual S. viminalis were rooted in containers filled with a mix of 25 kg of potting soil and 17.5 kg sand under a controlled environment (23/20°C, 60% relative humidity, 16/8h photoperiod). After three weeks, plantlets were transferred to individual pots and half of them were exposed to a mixture of heavy metals (1.5 ppm Cd, 175 ppm Cu, 30 ppm Ni, 500 ppm Zn). After 40 days, half of the plants were randomly chosen and cold-acclimated (7/5°C, 60% relative humidity, 16/8h photoperiod) while the remaining was kept under control conditions. A total of four temperature-polymetallic contamination combinations were obtained, namely Control soil Temperate (CT), Control soil Cold acclimated (CC), Metals-polluted soil Temperate (MT) and Metals-polluted soil Cold acclimated (MC). Three biological replicates per conditions were analysed using RNA-Seq approach</STUDY_ABSTRACT>
      <CENTER_PROJECT_NAME>GSE218490</CENTER_PROJECT_NAME>
    </DESCRIPTOR>
    <STUDY_LINKS>
      <STUDY_LINK>
        <XREF_LINK>
          <DB>pubmed</DB>
          <ID>38338824</ID>
        </XREF_LINK>
      </STUDY_LINK>
    </STUDY_LINKS>
  </STUDY>
</STUDY_SET>
