SRX18327736 GSM6745501_r1 SRP408937 PRJNA903876 SRS15816990 GSM6745501 GSM6745501 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA CD45+ cells freshyl isolated from the heart were washed in PBS and submitted for scRNA-seq library preparation using the 10X Genomics Platform. Following QC, the single cell suspension was loaded onto Chromium Chip B (10X Genomics PN 2000060) and GEM generation, cDNA synthesis, cDNA amplification, and library preparation of 4,500-9,300 cells proceeded using the Chromium Single Cell 3' Reagent Kit v3 (10X Genomics PN 1000075) according to the manufacturer's protocol. cDNA amplification included 11 cycles and 44-87ng of the material was used to prepare sequencing libraries with 12 cycles of PCR. Indexed libraries were pooled equimolar and sequenced on a NovaSeq 6000 in a PE28/91 paired end run using the NovaSeq 6000 SP, S1, or S2 Reagent Kit (100 cycles) (Illumina). An average of 201 million paired reads was generated per sample Illumina NovaSeq 6000 SRX18327737 GSM6745500_r1 SRP408937 PRJNA903876 SRS15816991 GSM6745500 GSM6745500 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA CD45+ cells freshyl isolated from the heart were washed in PBS and submitted for scRNA-seq library preparation using the 10X Genomics Platform. Following QC, the single cell suspension was loaded onto Chromium Chip B (10X Genomics PN 2000060) and GEM generation, cDNA synthesis, cDNA amplification, and library preparation of 4,500-9,300 cells proceeded using the Chromium Single Cell 3' Reagent Kit v3 (10X Genomics PN 1000075) according to the manufacturer's protocol. cDNA amplification included 11 cycles and 44-87ng of the material was used to prepare sequencing libraries with 12 cycles of PCR. Indexed libraries were pooled equimolar and sequenced on a NovaSeq 6000 in a PE28/91 paired end run using the NovaSeq 6000 SP, S1, or S2 Reagent Kit (100 cycles) (Illumina). An average of 201 million paired reads was generated per sample Illumina NovaSeq 6000 SRX18327738 GSM6745502_r1 SRP408937 PRJNA903876 SRS15816992 GSM6745502 GSM6745502 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA CD45+ cells freshyl isolated from the heart were washed in PBS and submitted for scRNA-seq library preparation using the 10X Genomics Platform. Following QC, the single cell suspension was loaded onto Chromium Chip B (10X Genomics PN 2000060) and GEM generation, cDNA synthesis, cDNA amplification, and library preparation of 4,500-9,300 cells proceeded using the Chromium Single Cell 3' Reagent Kit v3 (10X Genomics PN 1000075) according to the manufacturer's protocol. cDNA amplification included 11 cycles and 44-87ng of the material was used to prepare sequencing libraries with 12 cycles of PCR. Indexed libraries were pooled equimolar and sequenced on a NovaSeq 6000 in a PE28/91 paired end run using the NovaSeq 6000 SP, S1, or S2 Reagent Kit (100 cycles) (Illumina). An average of 201 million paired reads was generated per sample Illumina NovaSeq 6000 SRX18327739 GSM6745503_r1 SRP408937 PRJNA903876 SRS15816993 GSM6745503 GSM6745503 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA CD45+ cells freshyl isolated from the heart were washed in PBS and submitted for scRNA-seq library preparation using the 10X Genomics Platform. Following QC, the single cell suspension was loaded onto Chromium Chip B (10X Genomics PN 2000060) and GEM generation, cDNA synthesis, cDNA amplification, and library preparation of 4,500-9,300 cells proceeded using the Chromium Single Cell 3' Reagent Kit v3 (10X Genomics PN 1000075) according to the manufacturer's protocol. cDNA amplification included 11 cycles and 44-87ng of the material was used to prepare sequencing libraries with 12 cycles of PCR. Indexed libraries were pooled equimolar and sequenced on a NovaSeq 6000 in a PE28/91 paired end run using the NovaSeq 6000 SP, S1, or S2 Reagent Kit (100 cycles) (Illumina). An average of 201 million paired reads was generated per sample Illumina NovaSeq 6000 SRX18327740 GSM6745504_r1 SRP408937 PRJNA903876 SRS15816994 GSM6745504 GSM6745504 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA CD45+ cells freshyl isolated from the heart were washed in PBS and submitted for scRNA-seq library preparation using the 10X Genomics Platform. Following QC, the single cell suspension was loaded onto Chromium Chip B (10X Genomics PN 2000060) and GEM generation, cDNA synthesis, cDNA amplification, and library preparation of 4,500-9,300 cells proceeded using the Chromium Single Cell 3' Reagent Kit v3 (10X Genomics PN 1000075) according to the manufacturer's protocol. cDNA amplification included 11 cycles and 44-87ng of the material was used to prepare sequencing libraries with 12 cycles of PCR. Indexed libraries were pooled equimolar and sequenced on a NovaSeq 6000 in a PE28/91 paired end run using the NovaSeq 6000 SP, S1, or S2 Reagent Kit (100 cycles) (Illumina). An average of 201 million paired reads was generated per sample Illumina NovaSeq 6000 SRX18327741 GSM6745505_r1 SRP408937 PRJNA903876 SRS15816995 GSM6745505 GSM6745505 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA CD45+ cells freshyl isolated from the heart were washed in PBS and submitted for scRNA-seq library preparation using the 10X Genomics Platform. Following QC, the single cell suspension was loaded onto Chromium Chip B (10X Genomics PN 2000060) and GEM generation, cDNA synthesis, cDNA amplification, and library preparation of 4,500-9,300 cells proceeded using the Chromium Single Cell 3' Reagent Kit v3 (10X Genomics PN 1000075) according to the manufacturer's protocol. cDNA amplification included 11 cycles and 44-87ng of the material was used to prepare sequencing libraries with 12 cycles of PCR. Indexed libraries were pooled equimolar and sequenced on a NovaSeq 6000 in a PE28/91 paired end run using the NovaSeq 6000 SP, S1, or S2 Reagent Kit (100 cycles) (Illumina). An average of 201 million paired reads was generated per sample Illumina NovaSeq 6000