SRP409132 PRJNA904131 NGS data for fungal ITS1 region from seedlings, seeds, embryos, and callus tissues of bread wheat (Triticum aestivum) In this study we attempted to produce fungal endophyte free wheat (Triticum aestivum) plants using several methods: treatment of seeds with fungicides, production of plants from rescued embryos, and regeneration of plants from callus tissue.