SRX18337830 GSM6749144_r1 SRP409098 PRJNA904046 SRS15826669 GSM6749144 GSM6749144 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337831 GSM6749145_r1 SRP409098 PRJNA904046 SRS15826671 GSM6749145 GSM6749145 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337832 GSM6749146_r1 SRP409098 PRJNA904046 SRS15826670 GSM6749146 GSM6749146 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337833 GSM6749147_r1 SRP409098 PRJNA904046 SRS15826672 GSM6749147 GSM6749147 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337834 GSM6749148_r1 SRP409098 PRJNA904046 SRS15826673 GSM6749148 GSM6749148 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337835 GSM6749149_r1 SRP409098 PRJNA904046 SRS15826674 GSM6749149 GSM6749149 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337836 GSM6749150_r1 SRP409098 PRJNA904046 SRS15826675 GSM6749150 GSM6749150 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337837 GSM6749151_r1 SRP409098 PRJNA904046 SRS15826676 GSM6749151 GSM6749151 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337838 GSM6749152_r1 SRP409098 PRJNA904046 SRS15826677 GSM6749152 GSM6749152 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337839 GSM6749153_r1 SRP409098 PRJNA904046 SRS15826678 GSM6749153 GSM6749153 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337840 GSM6749154_r1 SRP409098 PRJNA904046 SRS15826679 GSM6749154 GSM6749154 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337841 GSM6749155_r1 SRP409098 PRJNA904046 SRS15826680 GSM6749155 GSM6749155 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337842 GSM6749156_r1 SRP409098 PRJNA904046 SRS15826681 GSM6749156 GSM6749156 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337843 GSM6749157_r1 SRP409098 PRJNA904046 SRS15826682 GSM6749157 GSM6749157 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337844 GSM6749158_r1 SRP409098 PRJNA904046 SRS15826683 GSM6749158 GSM6749158 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337845 GSM6749159_r1 SRP409098 PRJNA904046 SRS15826684 GSM6749159 GSM6749159 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337846 GSM6749184_r1 SRP409098 PRJNA904046 SRS15826685 GSM6749184 GSM6749184 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337847 GSM6749185_r1 SRP409098 PRJNA904046 SRS15826686 GSM6749185 GSM6749185 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337848 GSM6749186_r1 SRP409098 PRJNA904046 SRS15826687 GSM6749186 GSM6749186 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337849 GSM6749187_r1 SRP409098 PRJNA904046 SRS15826688 GSM6749187 GSM6749187 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337850 GSM6749188_r1 SRP409098 PRJNA904046 SRS15826689 GSM6749188 GSM6749188 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337851 GSM6749189_r1 SRP409098 PRJNA904046 SRS15826690 GSM6749189 GSM6749189 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337852 GSM6749190_r1 SRP409098 PRJNA904046 SRS15826691 GSM6749190 GSM6749190 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337853 GSM6749191_r1 SRP409098 PRJNA904046 SRS15826692 GSM6749191 GSM6749191 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337854 GSM6749160_r1 SRP409098 PRJNA904046 SRS15826693 GSM6749160 GSM6749160 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337855 GSM6749161_r1 SRP409098 PRJNA904046 SRS15826694 GSM6749161 GSM6749161 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337856 GSM6749162_r1 SRP409098 PRJNA904046 SRS15826695 GSM6749162 GSM6749162 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337857 GSM6749163_r1 SRP409098 PRJNA904046 SRS15826696 GSM6749163 GSM6749163 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337858 GSM6749164_r1 SRP409098 PRJNA904046 SRS15826697 GSM6749164 GSM6749164 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337859 GSM6749165_r1 SRP409098 PRJNA904046 SRS15826698 GSM6749165 GSM6749165 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337860 GSM6749166_r1 SRP409098 PRJNA904046 SRS15826699 GSM6749166 GSM6749166 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337861 GSM6749167_r1 SRP409098 PRJNA904046 SRS15826700 GSM6749167 GSM6749167 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337862 GSM6749192_r1 SRP409098 PRJNA904046 SRS15826701 GSM6749192 GSM6749192 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337863 GSM6749193_r1 SRP409098 PRJNA904046 SRS15826702 GSM6749193 GSM6749193 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337864 GSM6749194_r1 SRP409098 PRJNA904046 SRS15826703 GSM6749194 GSM6749194 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337865 GSM6749195_r1 SRP409098 PRJNA904046 SRS15826704 GSM6749195 GSM6749195 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337866 GSM6749196_r1 SRP409098 PRJNA904046 SRS15826705 GSM6749196 GSM6749196 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337867 GSM6749197_r1 SRP409098 PRJNA904046 SRS15826706 GSM6749197 GSM6749197 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337868 GSM6749198_r1 SRP409098 PRJNA904046 SRS15826707 GSM6749198 GSM6749198 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337869 GSM6749199_r1 SRP409098 PRJNA904046 SRS15826709 GSM6749199 GSM6749199 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337870 GSM6749168_r1 SRP409098 PRJNA904046 SRS15826708 GSM6749168 GSM6749168 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337871 GSM6749169_r1 SRP409098 PRJNA904046 SRS15826710 GSM6749169 GSM6749169 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337872 GSM6749170_r1 SRP409098 PRJNA904046 SRS15826711 GSM6749170 GSM6749170 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337873 GSM6749171_r1 SRP409098 PRJNA904046 SRS15826712 GSM6749171 GSM6749171 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337874 GSM6749172_r1 SRP409098 PRJNA904046 SRS15826714 GSM6749172 GSM6749172 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337875 GSM6749173_r1 SRP409098 PRJNA904046 SRS15826713 GSM6749173 GSM6749173 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337876 GSM6749174_r1 SRP409098 PRJNA904046 SRS15826715 GSM6749174 GSM6749174 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337877 GSM6749175_r1 SRP409098 PRJNA904046 SRS15826716 GSM6749175 GSM6749175 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337878 GSM6749200_r1 SRP409098 PRJNA904046 SRS15826717 GSM6749200 GSM6749200 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337879 GSM6749201_r1 SRP409098 PRJNA904046 SRS15826718 GSM6749201 GSM6749201 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337880 GSM6749202_r1 SRP409098 PRJNA904046 SRS15826719 GSM6749202 GSM6749202 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337881 GSM6749203_r1 SRP409098 PRJNA904046 SRS15826720 GSM6749203 GSM6749203 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337882 GSM6749204_r1 SRP409098 PRJNA904046 SRS15826721 GSM6749204 GSM6749204 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337883 GSM6749205_r1 SRP409098 PRJNA904046 SRS15826722 GSM6749205 GSM6749205 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337884 GSM6749206_r1 SRP409098 PRJNA904046 SRS15826723 GSM6749206 GSM6749206 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337885 GSM6749207_r1 SRP409098 PRJNA904046 SRS15826724 GSM6749207 GSM6749207 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337886 GSM6749176_r1 SRP409098 PRJNA904046 SRS15826725 GSM6749176 GSM6749176 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337887 GSM6749177_r1 SRP409098 PRJNA904046 SRS15826726 GSM6749177 GSM6749177 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337888 GSM6749178_r1 SRP409098 PRJNA904046 SRS15826727 GSM6749178 GSM6749178 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337889 GSM6749179_r1 SRP409098 PRJNA904046 SRS15826728 GSM6749179 GSM6749179 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337890 GSM6749180_r1 SRP409098 PRJNA904046 SRS15826729 GSM6749180 GSM6749180 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337891 GSM6749181_r1 SRP409098 PRJNA904046 SRS15826730 GSM6749181 GSM6749181 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337892 GSM6749182_r1 SRP409098 PRJNA904046 SRS15826731 GSM6749182 GSM6749182 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337893 GSM6749183_r1 SRP409098 PRJNA904046 SRS15826732 GSM6749183 GSM6749183 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337894 GSM6749208_r1 SRP409098 PRJNA904046 SRS15826733 GSM6749208 GSM6749208 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337895 GSM6749209_r1 SRP409098 PRJNA904046 SRS15826734 GSM6749209 GSM6749209 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337896 GSM6749210_r1 SRP409098 PRJNA904046 SRS15826735 GSM6749210 GSM6749210 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337897 GSM6749211_r1 SRP409098 PRJNA904046 SRS15826736 GSM6749211 GSM6749211 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337898 GSM6749212_r1 SRP409098 PRJNA904046 SRS15826737 GSM6749212 GSM6749212 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500 SRX18337899 GSM6749213_r1 SRP409098 PRJNA904046 SRS15826738 GSM6749213 GSM6749213 ChIP-Seq GENOMIC ChIP 500.000 to 1.000.000 cells were used for ChIP per sample. Cross-linking was done with 1% formaldehyde in DMEM added directly to cells in culture dishes for 8 min at room temperature. Quenching was performed using 0.1M glycine (final concentration). The cells were washed with ice-cold PBS and scrapped with PBS with protease inhibitor cocktail (Cat. 11697498001, Roche). Lysis was performed in LB1 buffer (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% glycerol, 0.5% Igepal CA-630, 0.25% Triton X100, protease inhibitors) by rotating for 10 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was incubated in LB2 buffer (10mM Tris-HCl pH8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, protease inhibitors) by rotating for 5 min at +4C, followed by centrifugation at 2000g for 5 min. The pellet was resuspended in a buffer containing 50mM Tris-HCl pH8.0, 10mM EDTA, 1% SDS, the sonication was performed for 45 cycles, 30-sec intervals on/off. Debris was removed by centrifugation, and 10% from the sonicated material was saved as input. Prior to immunoprecipitation, 5ug antibody and 100ul Protein A beads were pre-incubated overnight at +4C in a total volume of 250ul, adjusted with PBS with 5mg/ml BSA, followed by three washes using the same solution. Immunoprecipitation was done by combining the sonicated material with the bead-antibody complexes, in a total volume of 400ul adjusted with ChIP dilution buffer (10mM Tris-HCl pH8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% sodium deoxycholate, 0.5% N-lauroylsarcosine, 1% Triton X100, protease inhibitors), by rotating overnight at +4C. After the incubation, the beads were washed 6 times with RIPA buffer (50mM HEPES-KOH pH7.5, 500mM LiCl, 1mM EDTA, 1% Igepal CA-630, 0.7% sodium deoxycholate) followed by one wash in TE (10mM Tris-HCl pH8.0, 1mM EDTA). Reverse-crosslinking was done in elution buffer containing 1% SDS, 100mM sodium bicarbonate, and 200mM NaCl at 65C for 9-15 hours. The input sample was also reverse-crosslinked. After this step, the beads were removed from the mixture, and the remaining DNA in the solution was treated with 8 ug RNAse A for 1h at 37C, followed by 80ug proteinase K for 2 hours at 55C. DNA was purified using MinElute columns and then used for ChIP-Seq library preparation using the NEXTflex Rapid DNA-Seq Kit (NOVA-5144-02) according to the manufacturer's protocol with some minor modifications. Briefly, we amplified the final libraries with 4 cycles of PCR, then performed an AMPure XP-based size selection (0.5x to eliminate larger fragments followed by 1.8x to extract the smaller fragments), and then continued with 8 more PCR cycles on the size selected material. The final libraries were then cleaned up with 0.8x vol AMPure XP beads. Illumina HiSeq 2500