SRX18327096 GSM6744415_r1 SRP408910 PRJNA903804 SRS15816375 GSM6744415 GSM6744415 Bisulfite-Seq GENOMIC RANDOM For collection of meiotic spikelets with meiocytes at prophase of meiosis I, tillers whose flag leaf auricle was about 3 cm under the auricle of the penultimate leaf were chosen and 0.4-0.6 cm long fresh spikelets were collected. Meiotic spikelets collected above were put on a clean slide. The anthers were gently dissected out and collected using dissecting needles under the dissection microscope.The anthers were put on a clean slide with 5 µl DEPC water/1× PBS added. Under an inverted microscope (with 10× objective), a 1 ml syringe needle was use to dissect one end of the anthers. Because the meiocytes at this stage produce callose, they stick together and form worm-like cell clusters. Gentle pressure was applied to the anthers to allow the separation of wormlike cell clusters from anther walls. The cell clusters were collected with capillary glass pipettes without taking any somatic cells. scBS-seq libraries were constructed using a reported protocol (Clark et al., 2017). The library was constructed using the CUT&Tag kit (Hyperactive® Universal CUT&Tag Assay Kit for Illumina, Vazyme Biotech Co.,Ltd). Illumina NovaSeq 6000 SRX18327097 GSM6744416_r1 SRP408910 PRJNA903804 SRS15816376 GSM6744416 GSM6744416 Bisulfite-Seq GENOMIC RANDOM For collection of meiotic spikelets with meiocytes at prophase of meiosis I, tillers whose flag leaf auricle was about 3 cm under the auricle of the penultimate leaf were chosen and 0.4-0.6 cm long fresh spikelets were collected. Meiotic spikelets collected above were put on a clean slide. The anthers were gently dissected out and collected using dissecting needles under the dissection microscope.The anthers were put on a clean slide with 5 µl DEPC water/1× PBS added. Under an inverted microscope (with 10× objective), a 1 ml syringe needle was use to dissect one end of the anthers. Because the meiocytes at this stage produce callose, they stick together and form worm-like cell clusters. Gentle pressure was applied to the anthers to allow the separation of wormlike cell clusters from anther walls. The cell clusters were collected with capillary glass pipettes without taking any somatic cells. scBS-seq libraries were constructed using a reported protocol (Clark et al., 2017). The library was constructed using the CUT&Tag kit (Hyperactive® Universal CUT&Tag Assay Kit for Illumina, Vazyme Biotech Co.,Ltd). Illumina NovaSeq 6000 SRX18327098 GSM6744417_r1 SRP408910 PRJNA903804 SRS15816377 GSM6744417 GSM6744417 Bisulfite-Seq GENOMIC RANDOM For collection of meiotic spikelets with meiocytes at prophase of meiosis I, tillers whose flag leaf auricle was about 3 cm under the auricle of the penultimate leaf were chosen and 0.4-0.6 cm long fresh spikelets were collected. Meiotic spikelets collected above were put on a clean slide. The anthers were gently dissected out and collected using dissecting needles under the dissection microscope.The anthers were put on a clean slide with 5 µl DEPC water/1× PBS added. Under an inverted microscope (with 10× objective), a 1 ml syringe needle was use to dissect one end of the anthers. Because the meiocytes at this stage produce callose, they stick together and form worm-like cell clusters. Gentle pressure was applied to the anthers to allow the separation of wormlike cell clusters from anther walls. The cell clusters were collected with capillary glass pipettes without taking any somatic cells. scBS-seq libraries were constructed using a reported protocol (Clark et al., 2017). The library was constructed using the CUT&Tag kit (Hyperactive® Universal CUT&Tag Assay Kit for Illumina, Vazyme Biotech Co.,Ltd). Illumina NovaSeq 6000 SRX18327099 GSM6744418_r1 SRP408910 PRJNA903804 SRS15816378 GSM6744418 GSM6744418 Bisulfite-Seq GENOMIC RANDOM For collection of meiotic spikelets with meiocytes at prophase of meiosis I, tillers whose flag leaf auricle was about 3 cm under the auricle of the penultimate leaf were chosen and 0.4-0.6 cm long fresh spikelets were collected. Meiotic spikelets collected above were put on a clean slide. The anthers were gently dissected out and collected using dissecting needles under the dissection microscope.The anthers were put on a clean slide with 5 µl DEPC water/1× PBS added. Under an inverted microscope (with 10× objective), a 1 ml syringe needle was use to dissect one end of the anthers. Because the meiocytes at this stage produce callose, they stick together and form worm-like cell clusters. Gentle pressure was applied to the anthers to allow the separation of wormlike cell clusters from anther walls. The cell clusters were collected with capillary glass pipettes without taking any somatic cells. scBS-seq libraries were constructed using a reported protocol (Clark et al., 2017). The library was constructed using the CUT&Tag kit (Hyperactive® Universal CUT&Tag Assay Kit for Illumina, Vazyme Biotech Co.,Ltd). Illumina NovaSeq 6000 SRX18327100 GSM6744419_r1 SRP408910 PRJNA903804 SRS15816379 GSM6744419 GSM6744419 OTHER GENOMIC other For collection of meiotic spikelets with meiocytes at prophase of meiosis I, tillers whose flag leaf auricle was about 3 cm under the auricle of the penultimate leaf were chosen and 0.4-0.6 cm long fresh spikelets were collected. Meiotic spikelets collected above were put on a clean slide. The anthers were gently dissected out and collected using dissecting needles under the dissection microscope.The anthers were put on a clean slide with 5 µl DEPC water/1× PBS added. Under an inverted microscope (with 10× objective), a 1 ml syringe needle was use to dissect one end of the anthers. Because the meiocytes at this stage produce callose, they stick together and form worm-like cell clusters. Gentle pressure was applied to the anthers to allow the separation of wormlike cell clusters from anther walls. The cell clusters were collected with capillary glass pipettes without taking any somatic cells. scBS-seq libraries were constructed using a reported protocol (Clark et al., 2017). The library was constructed using the CUT&Tag kit (Hyperactive® Universal CUT&Tag Assay Kit for Illumina, Vazyme Biotech Co.,Ltd). Illumina NovaSeq 6000 SRX18327101 GSM6744420_r1 SRP408910 PRJNA903804 SRS15816380 GSM6744420 GSM6744420 OTHER GENOMIC other For collection of meiotic spikelets with meiocytes at prophase of meiosis I, tillers whose flag leaf auricle was about 3 cm under the auricle of the penultimate leaf were chosen and 0.4-0.6 cm long fresh spikelets were collected. Meiotic spikelets collected above were put on a clean slide. The anthers were gently dissected out and collected using dissecting needles under the dissection microscope.The anthers were put on a clean slide with 5 µl DEPC water/1× PBS added. Under an inverted microscope (with 10× objective), a 1 ml syringe needle was use to dissect one end of the anthers. Because the meiocytes at this stage produce callose, they stick together and form worm-like cell clusters. Gentle pressure was applied to the anthers to allow the separation of wormlike cell clusters from anther walls. The cell clusters were collected with capillary glass pipettes without taking any somatic cells. scBS-seq libraries were constructed using a reported protocol (Clark et al., 2017). The library was constructed using the CUT&Tag kit (Hyperactive® Universal CUT&Tag Assay Kit for Illumina, Vazyme Biotech Co.,Ltd). Illumina NovaSeq 6000 SRX18327102 GSM6744421_r1 SRP408910 PRJNA903804 SRS15816382 GSM6744421 GSM6744421 OTHER GENOMIC other For collection of meiotic spikelets with meiocytes at prophase of meiosis I, tillers whose flag leaf auricle was about 3 cm under the auricle of the penultimate leaf were chosen and 0.4-0.6 cm long fresh spikelets were collected. Meiotic spikelets collected above were put on a clean slide. The anthers were gently dissected out and collected using dissecting needles under the dissection microscope.The anthers were put on a clean slide with 5 µl DEPC water/1× PBS added. Under an inverted microscope (with 10× objective), a 1 ml syringe needle was use to dissect one end of the anthers. Because the meiocytes at this stage produce callose, they stick together and form worm-like cell clusters. Gentle pressure was applied to the anthers to allow the separation of wormlike cell clusters from anther walls. The cell clusters were collected with capillary glass pipettes without taking any somatic cells. scBS-seq libraries were constructed using a reported protocol (Clark et al., 2017). The library was constructed using the CUT&Tag kit (Hyperactive® Universal CUT&Tag Assay Kit for Illumina, Vazyme Biotech Co.,Ltd). Illumina NovaSeq 6000 SRX18327103 GSM6744422_r1 SRP408910 PRJNA903804 SRS15816381 GSM6744422 GSM6744422 OTHER GENOMIC other For collection of meiotic spikelets with meiocytes at prophase of meiosis I, tillers whose flag leaf auricle was about 3 cm under the auricle of the penultimate leaf were chosen and 0.4-0.6 cm long fresh spikelets were collected. Meiotic spikelets collected above were put on a clean slide. The anthers were gently dissected out and collected using dissecting needles under the dissection microscope.The anthers were put on a clean slide with 5 µl DEPC water/1× PBS added. Under an inverted microscope (with 10× objective), a 1 ml syringe needle was use to dissect one end of the anthers. Because the meiocytes at this stage produce callose, they stick together and form worm-like cell clusters. Gentle pressure was applied to the anthers to allow the separation of wormlike cell clusters from anther walls. The cell clusters were collected with capillary glass pipettes without taking any somatic cells. scBS-seq libraries were constructed using a reported protocol (Clark et al., 2017). The library was constructed using the CUT&Tag kit (Hyperactive® Universal CUT&Tag Assay Kit for Illumina, Vazyme Biotech Co.,Ltd). Illumina NovaSeq 6000 SRX18327104 GSM6744423_r1 SRP408910 PRJNA903804 SRS15816383 GSM6744423 GSM6744423 OTHER GENOMIC other For collection of meiotic spikelets with meiocytes at prophase of meiosis I, tillers whose flag leaf auricle was about 3 cm under the auricle of the penultimate leaf were chosen and 0.4-0.6 cm long fresh spikelets were collected. Meiotic spikelets collected above were put on a clean slide. The anthers were gently dissected out and collected using dissecting needles under the dissection microscope.The anthers were put on a clean slide with 5 µl DEPC water/1× PBS added. Under an inverted microscope (with 10× objective), a 1 ml syringe needle was use to dissect one end of the anthers. Because the meiocytes at this stage produce callose, they stick together and form worm-like cell clusters. Gentle pressure was applied to the anthers to allow the separation of wormlike cell clusters from anther walls. The cell clusters were collected with capillary glass pipettes without taking any somatic cells. scBS-seq libraries were constructed using a reported protocol (Clark et al., 2017). The library was constructed using the CUT&Tag kit (Hyperactive® Universal CUT&Tag Assay Kit for Illumina, Vazyme Biotech Co.,Ltd). Illumina NovaSeq 6000 SRX18327105 GSM6744424_r1 SRP408910 PRJNA903804 SRS15816384 GSM6744424 GSM6744424 OTHER GENOMIC other For collection of meiotic spikelets with meiocytes at prophase of meiosis I, tillers whose flag leaf auricle was about 3 cm under the auricle of the penultimate leaf were chosen and 0.4-0.6 cm long fresh spikelets were collected. Meiotic spikelets collected above were put on a clean slide. The anthers were gently dissected out and collected using dissecting needles under the dissection microscope.The anthers were put on a clean slide with 5 µl DEPC water/1× PBS added. Under an inverted microscope (with 10× objective), a 1 ml syringe needle was use to dissect one end of the anthers. Because the meiocytes at this stage produce callose, they stick together and form worm-like cell clusters. Gentle pressure was applied to the anthers to allow the separation of wormlike cell clusters from anther walls. The cell clusters were collected with capillary glass pipettes without taking any somatic cells. scBS-seq libraries were constructed using a reported protocol (Clark et al., 2017). The library was constructed using the CUT&Tag kit (Hyperactive® Universal CUT&Tag Assay Kit for Illumina, Vazyme Biotech Co.,Ltd). Illumina NovaSeq 6000 SRX18327106 GSM6744425_r1 SRP408910 PRJNA903804 SRS15816385 GSM6744425 GSM6744425 OTHER GENOMIC other For collection of meiotic spikelets with meiocytes at prophase of meiosis I, tillers whose flag leaf auricle was about 3 cm under the auricle of the penultimate leaf were chosen and 0.4-0.6 cm long fresh spikelets were collected. Meiotic spikelets collected above were put on a clean slide. The anthers were gently dissected out and collected using dissecting needles under the dissection microscope.The anthers were put on a clean slide with 5 µl DEPC water/1× PBS added. Under an inverted microscope (with 10× objective), a 1 ml syringe needle was use to dissect one end of the anthers. Because the meiocytes at this stage produce callose, they stick together and form worm-like cell clusters. Gentle pressure was applied to the anthers to allow the separation of wormlike cell clusters from anther walls. The cell clusters were collected with capillary glass pipettes without taking any somatic cells. scBS-seq libraries were constructed using a reported protocol (Clark et al., 2017). The library was constructed using the CUT&Tag kit (Hyperactive® Universal CUT&Tag Assay Kit for Illumina, Vazyme Biotech Co.,Ltd). Illumina NovaSeq 6000 SRX18327107 GSM6744426_r1 SRP408910 PRJNA903804 SRS15816386 GSM6744426 GSM6744426 OTHER GENOMIC other For collection of meiotic spikelets with meiocytes at prophase of meiosis I, tillers whose flag leaf auricle was about 3 cm under the auricle of the penultimate leaf were chosen and 0.4-0.6 cm long fresh spikelets were collected. Meiotic spikelets collected above were put on a clean slide. The anthers were gently dissected out and collected using dissecting needles under the dissection microscope.The anthers were put on a clean slide with 5 µl DEPC water/1× PBS added. Under an inverted microscope (with 10× objective), a 1 ml syringe needle was use to dissect one end of the anthers. Because the meiocytes at this stage produce callose, they stick together and form worm-like cell clusters. Gentle pressure was applied to the anthers to allow the separation of wormlike cell clusters from anther walls. The cell clusters were collected with capillary glass pipettes without taking any somatic cells. scBS-seq libraries were constructed using a reported protocol (Clark et al., 2017). The library was constructed using the CUT&Tag kit (Hyperactive® Universal CUT&Tag Assay Kit for Illumina, Vazyme Biotech Co.,Ltd). Illumina NovaSeq 6000 SRX18327108 GSM6744427_r1 SRP408910 PRJNA903804 SRS15816387 GSM6744427 GSM6744427 Bisulfite-Seq GENOMIC RANDOM For collection of meiotic spikelets with meiocytes at prophase of meiosis I, tillers whose flag leaf auricle was about 3 cm under the auricle of the penultimate leaf were chosen and 0.4-0.6 cm long fresh spikelets were collected. Meiotic spikelets collected above were put on a clean slide. The anthers were gently dissected out and collected using dissecting needles under the dissection microscope.The anthers were put on a clean slide with 5 µl DEPC water/1× PBS added. Under an inverted microscope (with 10× objective), a 1 ml syringe needle was use to dissect one end of the anthers. Because the meiocytes at this stage produce callose, they stick together and form worm-like cell clusters. Gentle pressure was applied to the anthers to allow the separation of wormlike cell clusters from anther walls. The cell clusters were collected with capillary glass pipettes without taking any somatic cells. scBS-seq libraries were constructed using a reported protocol (Clark et al., 2017). The library was constructed using the CUT&Tag kit (Hyperactive® Universal CUT&Tag Assay Kit for Illumina, Vazyme Biotech Co.,Ltd). Illumina NovaSeq 6000 SRX18327109 GSM6744428_r1 SRP408910 PRJNA903804 SRS15816388 GSM6744428 GSM6744428 Bisulfite-Seq GENOMIC RANDOM For collection of meiotic spikelets with meiocytes at prophase of meiosis I, tillers whose flag leaf auricle was about 3 cm under the auricle of the penultimate leaf were chosen and 0.4-0.6 cm long fresh spikelets were collected. Meiotic spikelets collected above were put on a clean slide. The anthers were gently dissected out and collected using dissecting needles under the dissection microscope.The anthers were put on a clean slide with 5 µl DEPC water/1× PBS added. Under an inverted microscope (with 10× objective), a 1 ml syringe needle was use to dissect one end of the anthers. Because the meiocytes at this stage produce callose, they stick together and form worm-like cell clusters. Gentle pressure was applied to the anthers to allow the separation of wormlike cell clusters from anther walls. The cell clusters were collected with capillary glass pipettes without taking any somatic cells. scBS-seq libraries were constructed using a reported protocol (Clark et al., 2017). The library was constructed using the CUT&Tag kit (Hyperactive® Universal CUT&Tag Assay Kit for Illumina, Vazyme Biotech Co.,Ltd). Illumina NovaSeq 6000 SRX18327110 GSM6744429_r1 SRP408910 PRJNA903804 SRS15816389 GSM6744429 GSM6744429 Bisulfite-Seq GENOMIC RANDOM For collection of meiotic spikelets with meiocytes at prophase of meiosis I, tillers whose flag leaf auricle was about 3 cm under the auricle of the penultimate leaf were chosen and 0.4-0.6 cm long fresh spikelets were collected. Meiotic spikelets collected above were put on a clean slide. The anthers were gently dissected out and collected using dissecting needles under the dissection microscope.The anthers were put on a clean slide with 5 µl DEPC water/1× PBS added. Under an inverted microscope (with 10× objective), a 1 ml syringe needle was use to dissect one end of the anthers. Because the meiocytes at this stage produce callose, they stick together and form worm-like cell clusters. Gentle pressure was applied to the anthers to allow the separation of wormlike cell clusters from anther walls. The cell clusters were collected with capillary glass pipettes without taking any somatic cells. scBS-seq libraries were constructed using a reported protocol (Clark et al., 2017). The library was constructed using the CUT&Tag kit (Hyperactive® Universal CUT&Tag Assay Kit for Illumina, Vazyme Biotech Co.,Ltd). Illumina NovaSeq 6000 SRX18327111 GSM6744430_r1 SRP408910 PRJNA903804 SRS15816390 GSM6744430 GSM6744430 Bisulfite-Seq GENOMIC RANDOM For collection of meiotic spikelets with meiocytes at prophase of meiosis I, tillers whose flag leaf auricle was about 3 cm under the auricle of the penultimate leaf were chosen and 0.4-0.6 cm long fresh spikelets were collected. Meiotic spikelets collected above were put on a clean slide. The anthers were gently dissected out and collected using dissecting needles under the dissection microscope.The anthers were put on a clean slide with 5 µl DEPC water/1× PBS added. Under an inverted microscope (with 10× objective), a 1 ml syringe needle was use to dissect one end of the anthers. Because the meiocytes at this stage produce callose, they stick together and form worm-like cell clusters. Gentle pressure was applied to the anthers to allow the separation of wormlike cell clusters from anther walls. The cell clusters were collected with capillary glass pipettes without taking any somatic cells. scBS-seq libraries were constructed using a reported protocol (Clark et al., 2017). The library was constructed using the CUT&Tag kit (Hyperactive® Universal CUT&Tag Assay Kit for Illumina, Vazyme Biotech Co.,Ltd). Illumina NovaSeq 6000