SRX18358749 GSM6753257_r1 SRP409384 PRJNA904656 SRS15845481 GSM6753257 GSM6753257 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA brains were extracted followed by isolation of nuclei using FACS. For experiments utilizing the 10x Genomics platform, the following reagents were used: Chromium Next GEM Single Cell 3′ Library kit v3.1 (PN-1000158), Chromium Next GEM Single Cell 3′ GEM Kit v3.1 (PN-1000130), Chromium Next GEM Single Cell 3' Gel Bead Kit v3.1 (PN-1000129) and Dynabeads MyOne SILANE (PN-2000048) and were used according to the manufacturer's instructions in the Chromium Single Cell 3′ Reagents Kits V3.1 User Guide. Single nuclei droplets were generated using 10X Genomics Droplet generator and in total 10,000 nuclei per sample were targeted. Sequencing and read mapping: Generated libraries were sequenced on an Illumina NextSeq 500 at the Next Generation Sequencing Facility[ZS1] of the Max Planck Institute of Biochemistry with >50 x 103 reads per nucleus followed by de-multiplexing and mapping to the mouse genome (build mm10) using CellRanger v3.0 (10x Genomics) with standard settings and inclusion of introns for nuclear transcripts. Count matrixes were further analyzed with Seurat V4.2.0 R package NextSeq 500 SRX18358750 GSM6753258_r1 SRP409384 PRJNA904656 SRS15845482 GSM6753258 GSM6753258 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA brains were extracted followed by isolation of nuclei using FACS. For experiments utilizing the 10x Genomics platform, the following reagents were used: Chromium Next GEM Single Cell 3′ Library kit v3.1 (PN-1000158), Chromium Next GEM Single Cell 3′ GEM Kit v3.1 (PN-1000130), Chromium Next GEM Single Cell 3' Gel Bead Kit v3.1 (PN-1000129) and Dynabeads MyOne SILANE (PN-2000048) and were used according to the manufacturer's instructions in the Chromium Single Cell 3′ Reagents Kits V3.1 User Guide. Single nuclei droplets were generated using 10X Genomics Droplet generator and in total 10,000 nuclei per sample were targeted. Sequencing and read mapping: Generated libraries were sequenced on an Illumina NextSeq 500 at the Next Generation Sequencing Facility[ZS1] of the Max Planck Institute of Biochemistry with >50 x 103 reads per nucleus followed by de-multiplexing and mapping to the mouse genome (build mm10) using CellRanger v3.0 (10x Genomics) with standard settings and inclusion of introns for nuclear transcripts. Count matrixes were further analyzed with Seurat V4.2.0 R package NextSeq 500 SRX18358751 GSM6753259_r1 SRP409384 PRJNA904656 SRS15845483 GSM6753259 GSM6753259 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA brains were extracted followed by isolation of nuclei using FACS. For experiments utilizing the 10x Genomics platform, the following reagents were used: Chromium Next GEM Single Cell 3′ Library kit v3.1 (PN-1000158), Chromium Next GEM Single Cell 3′ GEM Kit v3.1 (PN-1000130), Chromium Next GEM Single Cell 3' Gel Bead Kit v3.1 (PN-1000129) and Dynabeads MyOne SILANE (PN-2000048) and were used according to the manufacturer's instructions in the Chromium Single Cell 3′ Reagents Kits V3.1 User Guide. Single nuclei droplets were generated using 10X Genomics Droplet generator and in total 10,000 nuclei per sample were targeted. Sequencing and read mapping: Generated libraries were sequenced on an Illumina NextSeq 500 at the Next Generation Sequencing Facility[ZS1] of the Max Planck Institute of Biochemistry with >50 x 103 reads per nucleus followed by de-multiplexing and mapping to the mouse genome (build mm10) using CellRanger v3.0 (10x Genomics) with standard settings and inclusion of introns for nuclear transcripts. Count matrixes were further analyzed with Seurat V4.2.0 R package NextSeq 500 SRX18358752 GSM6753260_r1 SRP409384 PRJNA904656 SRS15845484 GSM6753260 GSM6753260 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA brains were extracted followed by isolation of nuclei using FACS. For experiments utilizing the 10x Genomics platform, the following reagents were used: Chromium Next GEM Single Cell 3′ Library kit v3.1 (PN-1000158), Chromium Next GEM Single Cell 3′ GEM Kit v3.1 (PN-1000130), Chromium Next GEM Single Cell 3' Gel Bead Kit v3.1 (PN-1000129) and Dynabeads MyOne SILANE (PN-2000048) and were used according to the manufacturer's instructions in the Chromium Single Cell 3′ Reagents Kits V3.1 User Guide. Single nuclei droplets were generated using 10X Genomics Droplet generator and in total 10,000 nuclei per sample were targeted. Sequencing and read mapping: Generated libraries were sequenced on an Illumina NextSeq 500 at the Next Generation Sequencing Facility[ZS1] of the Max Planck Institute of Biochemistry with >50 x 103 reads per nucleus followed by de-multiplexing and mapping to the mouse genome (build mm10) using CellRanger v3.0 (10x Genomics) with standard settings and inclusion of introns for nuclear transcripts. Count matrixes were further analyzed with Seurat V4.2.0 R package NextSeq 500 SRX18358753 GSM6753261_r1 SRP409384 PRJNA904656 SRS15845485 GSM6753261 GSM6753261 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA brains were extracted followed by isolation of nuclei using FACS. For experiments utilizing the 10x Genomics platform, the following reagents were used: Chromium Next GEM Single Cell 3′ Library kit v3.1 (PN-1000158), Chromium Next GEM Single Cell 3′ GEM Kit v3.1 (PN-1000130), Chromium Next GEM Single Cell 3' Gel Bead Kit v3.1 (PN-1000129) and Dynabeads MyOne SILANE (PN-2000048) and were used according to the manufacturer's instructions in the Chromium Single Cell 3′ Reagents Kits V3.1 User Guide. Single nuclei droplets were generated using 10X Genomics Droplet generator and in total 10,000 nuclei per sample were targeted. Sequencing and read mapping: Generated libraries were sequenced on an Illumina NextSeq 500 at the Next Generation Sequencing Facility[ZS1] of the Max Planck Institute of Biochemistry with >50 x 103 reads per nucleus followed by de-multiplexing and mapping to the mouse genome (build mm10) using CellRanger v3.0 (10x Genomics) with standard settings and inclusion of introns for nuclear transcripts. Count matrixes were further analyzed with Seurat V4.2.0 R package NextSeq 500 SRX18358754 GSM6753262_r1 SRP409384 PRJNA904656 SRS15845486 GSM6753262 GSM6753262 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA brains were extracted followed by isolation of nuclei using FACS. For experiments utilizing the 10x Genomics platform, the following reagents were used: Chromium Next GEM Single Cell 3′ Library kit v3.1 (PN-1000158), Chromium Next GEM Single Cell 3′ GEM Kit v3.1 (PN-1000130), Chromium Next GEM Single Cell 3' Gel Bead Kit v3.1 (PN-1000129) and Dynabeads MyOne SILANE (PN-2000048) and were used according to the manufacturer's instructions in the Chromium Single Cell 3′ Reagents Kits V3.1 User Guide. Single nuclei droplets were generated using 10X Genomics Droplet generator and in total 10,000 nuclei per sample were targeted. Sequencing and read mapping: Generated libraries were sequenced on an Illumina NextSeq 500 at the Next Generation Sequencing Facility[ZS1] of the Max Planck Institute of Biochemistry with >50 x 103 reads per nucleus followed by de-multiplexing and mapping to the mouse genome (build mm10) using CellRanger v3.0 (10x Genomics) with standard settings and inclusion of introns for nuclear transcripts. Count matrixes were further analyzed with Seurat V4.2.0 R package NextSeq 500