<?xml version="1.0" encoding="UTF-8"?>
<EXPERIMENT_SET xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
  <EXPERIMENT accession="SRX18363590" alias="GSM6753921_r1">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX18363590</PRIMARY_ID>
      <EXTERNAL_ID namespace="GEO">GSM6753921_r1</EXTERNAL_ID>
    </IDENTIFIERS>
    <TITLE>GSM6753921: Cx-6-10, 6-months old, cortex; Mus musculus; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP409471">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP409471</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA904737</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS15850048">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS15850048</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM6753921</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>GSM6753921</LIBRARY_NAME>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Cortex powder was first homogenized in 500µL of cold TRIzol and then 500µL of chloroform:isoamyl alcohol 24:1 (Sigma Aldrich) were added. Samples were vortexed for 20 seconds and incubated 2 min at room temperature. The samples were then centrifuged at 10,000g for 18 min at 4°C. The aqueous phase, that contains RNAs, was transferred in a new Eppendorf tube. An equal volume of ethanol 100% was added and the mixture was then loaded into a Zymo-Spin™ IIICG Column from the Quick-RNA™ Miniprep Kit. Further steps were conducted according to the manufacturer's protocol. The RNA concentration in ng/µL was determined using a Nanodrop system (Thermo Fisher Sscientific). Library preparation was initiated with 10ng of total RNA. RNA-seq libraries were prepared using NEBNext® single cell/low input RNA library preparation Kit (New England Biolabs).</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>NextSeq 500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX18363591" alias="GSM6753922_r1">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX18363591</PRIMARY_ID>
      <EXTERNAL_ID namespace="GEO">GSM6753922_r1</EXTERNAL_ID>
    </IDENTIFIERS>
    <TITLE>GSM6753922: Cx-6-11, 6-months old, cortex; Mus musculus; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP409471">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP409471</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA904737</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS15850049">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS15850049</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM6753922</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>GSM6753922</LIBRARY_NAME>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Cortex powder was first homogenized in 500µL of cold TRIzol and then 500µL of chloroform:isoamyl alcohol 24:1 (Sigma Aldrich) were added. Samples were vortexed for 20 seconds and incubated 2 min at room temperature. The samples were then centrifuged at 10,000g for 18 min at 4°C. The aqueous phase, that contains RNAs, was transferred in a new Eppendorf tube. An equal volume of ethanol 100% was added and the mixture was then loaded into a Zymo-Spin™ IIICG Column from the Quick-RNA™ Miniprep Kit. Further steps were conducted according to the manufacturer's protocol. The RNA concentration in ng/µL was determined using a Nanodrop system (Thermo Fisher Sscientific). Library preparation was initiated with 10ng of total RNA. RNA-seq libraries were prepared using NEBNext® single cell/low input RNA library preparation Kit (New England Biolabs).</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>NextSeq 500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX18363592" alias="GSM6753923_r1">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX18363592</PRIMARY_ID>
      <EXTERNAL_ID namespace="GEO">GSM6753923_r1</EXTERNAL_ID>
    </IDENTIFIERS>
    <TITLE>GSM6753923: Cx-6-17, 6-months old, cortex; Mus musculus; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP409471">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP409471</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA904737</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS15850050">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS15850050</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM6753923</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>GSM6753923</LIBRARY_NAME>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Cortex powder was first homogenized in 500µL of cold TRIzol and then 500µL of chloroform:isoamyl alcohol 24:1 (Sigma Aldrich) were added. Samples were vortexed for 20 seconds and incubated 2 min at room temperature. The samples were then centrifuged at 10,000g for 18 min at 4°C. The aqueous phase, that contains RNAs, was transferred in a new Eppendorf tube. An equal volume of ethanol 100% was added and the mixture was then loaded into a Zymo-Spin™ IIICG Column from the Quick-RNA™ Miniprep Kit. Further steps were conducted according to the manufacturer's protocol. The RNA concentration in ng/µL was determined using a Nanodrop system (Thermo Fisher Sscientific). Library preparation was initiated with 10ng of total RNA. RNA-seq libraries were prepared using NEBNext® single cell/low input RNA library preparation Kit (New England Biolabs).</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>NextSeq 500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX18363593" alias="GSM6753924_r1">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX18363593</PRIMARY_ID>
      <EXTERNAL_ID namespace="GEO">GSM6753924_r1</EXTERNAL_ID>
    </IDENTIFIERS>
    <TITLE>GSM6753924: Cx-24-2, 24-months old, cortex; Mus musculus; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP409471">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP409471</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA904737</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS15850051">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS15850051</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM6753924</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>GSM6753924</LIBRARY_NAME>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Cortex powder was first homogenized in 500µL of cold TRIzol and then 500µL of chloroform:isoamyl alcohol 24:1 (Sigma Aldrich) were added. Samples were vortexed for 20 seconds and incubated 2 min at room temperature. The samples were then centrifuged at 10,000g for 18 min at 4°C. The aqueous phase, that contains RNAs, was transferred in a new Eppendorf tube. An equal volume of ethanol 100% was added and the mixture was then loaded into a Zymo-Spin™ IIICG Column from the Quick-RNA™ Miniprep Kit. Further steps were conducted according to the manufacturer's protocol. The RNA concentration in ng/µL was determined using a Nanodrop system (Thermo Fisher Sscientific). Library preparation was initiated with 10ng of total RNA. RNA-seq libraries were prepared using NEBNext® single cell/low input RNA library preparation Kit (New England Biolabs).</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>NextSeq 500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX18363594" alias="GSM6753925_r1">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX18363594</PRIMARY_ID>
      <EXTERNAL_ID namespace="GEO">GSM6753925_r1</EXTERNAL_ID>
    </IDENTIFIERS>
    <TITLE>GSM6753925: Cx-24-4, 24-months old, cortex; Mus musculus; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP409471">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP409471</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA904737</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS15850052">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS15850052</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM6753925</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>GSM6753925</LIBRARY_NAME>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Cortex powder was first homogenized in 500µL of cold TRIzol and then 500µL of chloroform:isoamyl alcohol 24:1 (Sigma Aldrich) were added. Samples were vortexed for 20 seconds and incubated 2 min at room temperature. The samples were then centrifuged at 10,000g for 18 min at 4°C. The aqueous phase, that contains RNAs, was transferred in a new Eppendorf tube. An equal volume of ethanol 100% was added and the mixture was then loaded into a Zymo-Spin™ IIICG Column from the Quick-RNA™ Miniprep Kit. Further steps were conducted according to the manufacturer's protocol. The RNA concentration in ng/µL was determined using a Nanodrop system (Thermo Fisher Sscientific). Library preparation was initiated with 10ng of total RNA. RNA-seq libraries were prepared using NEBNext® single cell/low input RNA library preparation Kit (New England Biolabs).</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>NextSeq 500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
  <EXPERIMENT accession="SRX18363595" alias="GSM6753926_r1">
    <IDENTIFIERS>
      <PRIMARY_ID>SRX18363595</PRIMARY_ID>
      <EXTERNAL_ID namespace="GEO">GSM6753926_r1</EXTERNAL_ID>
    </IDENTIFIERS>
    <TITLE>GSM6753926: Cx-24-5, 24-months old, cortex; Mus musculus; RNA-Seq</TITLE>
    <STUDY_REF accession="SRP409471">
      <IDENTIFIERS>
        <PRIMARY_ID>SRP409471</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioProject">PRJNA904737</EXTERNAL_ID>
      </IDENTIFIERS>
    </STUDY_REF>
    <DESIGN>
      <DESIGN_DESCRIPTION/>
      <SAMPLE_DESCRIPTOR accession="SRS15850053">
        <IDENTIFIERS>
          <PRIMARY_ID>SRS15850053</PRIMARY_ID>
          <EXTERNAL_ID namespace="GEO">GSM6753926</EXTERNAL_ID>
        </IDENTIFIERS>
      </SAMPLE_DESCRIPTOR>
      <LIBRARY_DESCRIPTOR>
        <LIBRARY_NAME>GSM6753926</LIBRARY_NAME>
        <LIBRARY_STRATEGY>RNA-Seq</LIBRARY_STRATEGY>
        <LIBRARY_SOURCE>TRANSCRIPTOMIC</LIBRARY_SOURCE>
        <LIBRARY_SELECTION>cDNA</LIBRARY_SELECTION>
        <LIBRARY_LAYOUT>
          <SINGLE/>
        </LIBRARY_LAYOUT>
        <LIBRARY_CONSTRUCTION_PROTOCOL>Cortex powder was first homogenized in 500µL of cold TRIzol and then 500µL of chloroform:isoamyl alcohol 24:1 (Sigma Aldrich) were added. Samples were vortexed for 20 seconds and incubated 2 min at room temperature. The samples were then centrifuged at 10,000g for 18 min at 4°C. The aqueous phase, that contains RNAs, was transferred in a new Eppendorf tube. An equal volume of ethanol 100% was added and the mixture was then loaded into a Zymo-Spin™ IIICG Column from the Quick-RNA™ Miniprep Kit. Further steps were conducted according to the manufacturer's protocol. The RNA concentration in ng/µL was determined using a Nanodrop system (Thermo Fisher Sscientific). Library preparation was initiated with 10ng of total RNA. RNA-seq libraries were prepared using NEBNext® single cell/low input RNA library preparation Kit (New England Biolabs).</LIBRARY_CONSTRUCTION_PROTOCOL>
      </LIBRARY_DESCRIPTOR>
    </DESIGN>
    <PLATFORM>
      <ILLUMINA>
        <INSTRUMENT_MODEL>NextSeq 500</INSTRUMENT_MODEL>
      </ILLUMINA>
    </PLATFORM>
  </EXPERIMENT>
</EXPERIMENT_SET>
