SRX18366919 GSM6754171_r1 SRP409511 PRJNA904769 SRS15853352 GSM6754171 GSM6754171 MNase-Seq GENOMIC MNase 1 x 106 cells were harvested and transferred into lysis buffer. Nuclei were then pelleted, washed and resuspended in digestion buffer containing 2 mM CaCl2 and 2,500 gel units micrococcal nuclease (NEB, M0247S) with shaking at 37℃ for 5 min. The reaction was stopped by EDTA and protease was added to digest proteins. Then fragmented and naked genomic DNA were released. The sequencing libraries were prepared from 10 ng of purified mono-nucleosome-DNAs using the VAHTS® Universal DNA Library Prep Kit (Vazyme, ND607) with 10 cycles of PCR. Illumina NovaSeq 6000 SRX18366920 GSM6754172_r1 SRP409511 PRJNA904769 SRS15853353 GSM6754172 GSM6754172 MNase-Seq GENOMIC MNase 1 x 106 cells were harvested and transferred into lysis buffer. Nuclei were then pelleted, washed and resuspended in digestion buffer containing 2 mM CaCl2 and 2,500 gel units micrococcal nuclease (NEB, M0247S) with shaking at 37℃ for 5 min. The reaction was stopped by EDTA and protease was added to digest proteins. Then fragmented and naked genomic DNA were released. The sequencing libraries were prepared from 10 ng of purified mono-nucleosome-DNAs using the VAHTS® Universal DNA Library Prep Kit (Vazyme, ND607) with 10 cycles of PCR. Illumina NovaSeq 6000 SRX18366921 GSM6754173_r1 SRP409511 PRJNA904769 SRS15853354 GSM6754173 GSM6754173 MNase-Seq GENOMIC MNase 1 x 106 cells were harvested and transferred into lysis buffer. Nuclei were then pelleted, washed and resuspended in digestion buffer containing 2 mM CaCl2 and 2,500 gel units micrococcal nuclease (NEB, M0247S) with shaking at 37℃ for 5 min. The reaction was stopped by EDTA and protease was added to digest proteins. Then fragmented and naked genomic DNA were released. The sequencing libraries were prepared from 10 ng of purified mono-nucleosome-DNAs using the VAHTS® Universal DNA Library Prep Kit (Vazyme, ND607) with 10 cycles of PCR. Illumina NovaSeq 6000 SRX18366922 GSM6754174_r1 SRP409511 PRJNA904769 SRS15853355 GSM6754174 GSM6754174 MNase-Seq GENOMIC MNase 1 x 106 cells were harvested and transferred into lysis buffer. Nuclei were then pelleted, washed and resuspended in digestion buffer containing 2 mM CaCl2 and 2,500 gel units micrococcal nuclease (NEB, M0247S) with shaking at 37℃ for 5 min. The reaction was stopped by EDTA and protease was added to digest proteins. Then fragmented and naked genomic DNA were released. The sequencing libraries were prepared from 10 ng of purified mono-nucleosome-DNAs using the VAHTS® Universal DNA Library Prep Kit (Vazyme, ND607) with 10 cycles of PCR. Illumina NovaSeq 6000