SRX18390977 femvitro4 SRP410059 PRJNA905798 Genomic DNA extraction was performed by the Nucleospin Tissue XS kit (Macherey-Nagel) after washing tick samples in 1% bleach solution and tick tissue preparation. After quality control, DNA was diluted to 1 ng/ L. To amplicon sequencing of the V3-V4 region of bacterial 16S rRNA was performed by primers 341F (5-CCTAYGGGRBGCASCAG-3) and 806R (5-GGACTACNNGGGTATCTAAT-3). The PCR was performed using Phusion High-Fidelity PCR Master Mix (New England Biolabs) followed by agarose gel electrophoresis. PCR product mixtures were purified using the Qiagen Gel Extraction kit (Qiagen, Germany). Libraries were genereted by NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) and quantified by Qubit and qPCR. Sequencing depth was set as 50k raw reads per sample. SRS15875091 SAMN31871511 femvitro4 AMPLICON METAGENOMIC PCR Illumina NovaSeq 6000 SRX18390978 femvitroAB1 SRP410059 PRJNA905798 Genomic DNA extraction was performed by the Nucleospin Tissue XS kit (Macherey-Nagel) after washing tick samples in 1% bleach solution and tick tissue preparation. After quality control, DNA was diluted to 1 ng/ L. To amplicon sequencing of the V3-V4 region of bacterial 16S rRNA was performed by primers 341F (5-CCTAYGGGRBGCASCAG-3) and 806R (5-GGACTACNNGGGTATCTAAT-3). The PCR was performed using Phusion High-Fidelity PCR Master Mix (New England Biolabs) followed by agarose gel electrophoresis. PCR product mixtures were purified using the Qiagen Gel Extraction kit (Qiagen, Germany). Libraries were genereted by NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) and quantified by Qubit and qPCR. Sequencing depth was set as 50k raw reads per sample. SRS15875092 SAMN31871512 femvitroAB1 AMPLICON METAGENOMIC PCR Illumina NovaSeq 6000 SRX18390979 femvivo5 SRP410059 PRJNA905798 Genomic DNA extraction was performed by the Nucleospin Tissue XS kit (Macherey-Nagel) after washing tick samples in 1% bleach solution and tick tissue preparation. After quality control, DNA was diluted to 1 ng/ L. To amplicon sequencing of the V3-V4 region of bacterial 16S rRNA was performed by primers 341F (5-CCTAYGGGRBGCASCAG-3) and 806R (5-GGACTACNNGGGTATCTAAT-3). The PCR was performed using Phusion High-Fidelity PCR Master Mix (New England Biolabs) followed by agarose gel electrophoresis. PCR product mixtures were purified using the Qiagen Gel Extraction kit (Qiagen, Germany). Libraries were genereted by NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) and quantified by Qubit and qPCR. Sequencing depth was set as 50k raw reads per sample. SRS15875093 SAMN31871521 femvivo5 AMPLICON METAGENOMIC PCR Illumina NovaSeq 6000 SRX18390980 femvivo6 SRP410059 PRJNA905798 Genomic DNA extraction was performed by the Nucleospin Tissue XS kit (Macherey-Nagel) after washing tick samples in 1% bleach solution and tick tissue preparation. After quality control, DNA was diluted to 1 ng/ L. To amplicon sequencing of the V3-V4 region of bacterial 16S rRNA was performed by primers 341F (5-CCTAYGGGRBGCASCAG-3) and 806R (5-GGACTACNNGGGTATCTAAT-3). The PCR was performed using Phusion High-Fidelity PCR Master Mix (New England Biolabs) followed by agarose gel electrophoresis. PCR product mixtures were purified using the Qiagen Gel Extraction kit (Qiagen, Germany). Libraries were genereted by NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) and quantified by Qubit and qPCR. Sequencing depth was set as 50k raw reads per sample. SRS15875094 SAMN31871522 femvivo6 AMPLICON METAGENOMIC PCR Illumina NovaSeq 6000 SRX18390981 femvivo7 SRP410059 PRJNA905798 Genomic DNA extraction was performed by the Nucleospin Tissue XS kit (Macherey-Nagel) after washing tick samples in 1% bleach solution and tick tissue preparation. After quality control, DNA was diluted to 1 ng/ L. To amplicon sequencing of the V3-V4 region of bacterial 16S rRNA was performed by primers 341F (5-CCTAYGGGRBGCASCAG-3) and 806R (5-GGACTACNNGGGTATCTAAT-3). The PCR was performed using Phusion High-Fidelity PCR Master Mix (New England Biolabs) followed by agarose gel electrophoresis. PCR product mixtures were purified using the Qiagen Gel Extraction kit (Qiagen, Germany). Libraries were genereted by NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) and quantified by Qubit and qPCR. Sequencing depth was set as 50k raw reads per sample. SRS15875095 SAMN31871523 femvivo7 AMPLICON METAGENOMIC PCR Illumina NovaSeq 6000 SRX18390982 femvivo8 SRP410059 PRJNA905798 Genomic DNA extraction was performed by the Nucleospin Tissue XS kit (Macherey-Nagel) after washing tick samples in 1% bleach solution and tick tissue preparation. After quality control, DNA was diluted to 1 ng/ L. To amplicon sequencing of the V3-V4 region of bacterial 16S rRNA was performed by primers 341F (5-CCTAYGGGRBGCASCAG-3) and 806R (5-GGACTACNNGGGTATCTAAT-3). The PCR was performed using Phusion High-Fidelity PCR Master Mix (New England Biolabs) followed by agarose gel electrophoresis. PCR product mixtures were purified using the Qiagen Gel Extraction kit (Qiagen, Germany). Libraries were genereted by NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) and quantified by Qubit and qPCR. Sequencing depth was set as 50k raw reads per sample. SRS15875096 SAMN31871524 femvivo8 AMPLICON METAGENOMIC PCR Illumina NovaSeq 6000 SRX18390983 femvitroAB2 SRP410059 PRJNA905798 Genomic DNA extraction was performed by the Nucleospin Tissue XS kit (Macherey-Nagel) after washing tick samples in 1% bleach solution and tick tissue preparation. After quality control, DNA was diluted to 1 ng/ L. To amplicon sequencing of the V3-V4 region of bacterial 16S rRNA was performed by primers 341F (5-CCTAYGGGRBGCASCAG-3) and 806R (5-GGACTACNNGGGTATCTAAT-3). The PCR was performed using Phusion High-Fidelity PCR Master Mix (New England Biolabs) followed by agarose gel electrophoresis. PCR product mixtures were purified using the Qiagen Gel Extraction kit (Qiagen, Germany). Libraries were genereted by NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) and quantified by Qubit and qPCR. Sequencing depth was set as 50k raw reads per sample. SRS15875097 SAMN31871513 femvitroAB2 AMPLICON METAGENOMIC PCR Illumina NovaSeq 6000 SRX18390984 femvitroAB3 SRP410059 PRJNA905798 Genomic DNA extraction was performed by the Nucleospin Tissue XS kit (Macherey-Nagel) after washing tick samples in 1% bleach solution and tick tissue preparation. After quality control, DNA was diluted to 1 ng/ L. To amplicon sequencing of the V3-V4 region of bacterial 16S rRNA was performed by primers 341F (5-CCTAYGGGRBGCASCAG-3) and 806R (5-GGACTACNNGGGTATCTAAT-3). The PCR was performed using Phusion High-Fidelity PCR Master Mix (New England Biolabs) followed by agarose gel electrophoresis. PCR product mixtures were purified using the Qiagen Gel Extraction kit (Qiagen, Germany). Libraries were genereted by NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) and quantified by Qubit and qPCR. Sequencing depth was set as 50k raw reads per sample. SRS15875098 SAMN31871514 femvitroAB3 AMPLICON METAGENOMIC PCR Illumina NovaSeq 6000 SRX18390985 femvitroAB4 SRP410059 PRJNA905798 Genomic DNA extraction was performed by the Nucleospin Tissue XS kit (Macherey-Nagel) after washing tick samples in 1% bleach solution and tick tissue preparation. After quality control, DNA was diluted to 1 ng/ L. To amplicon sequencing of the V3-V4 region of bacterial 16S rRNA was performed by primers 341F (5-CCTAYGGGRBGCASCAG-3) and 806R (5-GGACTACNNGGGTATCTAAT-3). The PCR was performed using Phusion High-Fidelity PCR Master Mix (New England Biolabs) followed by agarose gel electrophoresis. PCR product mixtures were purified using the Qiagen Gel Extraction kit (Qiagen, Germany). Libraries were genereted by NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) and quantified by Qubit and qPCR. Sequencing depth was set as 50k raw reads per sample. SRS15875099 SAMN31871515 femvitroAB4 AMPLICON METAGENOMIC PCR Illumina NovaSeq 6000 SRX18390986 femvitroAB5 SRP410059 PRJNA905798 Genomic DNA extraction was performed by the Nucleospin Tissue XS kit (Macherey-Nagel) after washing tick samples in 1% bleach solution and tick tissue preparation. After quality control, DNA was diluted to 1 ng/ L. To amplicon sequencing of the V3-V4 region of bacterial 16S rRNA was performed by primers 341F (5-CCTAYGGGRBGCASCAG-3) and 806R (5-GGACTACNNGGGTATCTAAT-3). The PCR was performed using Phusion High-Fidelity PCR Master Mix (New England Biolabs) followed by agarose gel electrophoresis. PCR product mixtures were purified using the Qiagen Gel Extraction kit (Qiagen, Germany). Libraries were genereted by NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) and quantified by Qubit and qPCR. Sequencing depth was set as 50k raw reads per sample. SRS15875100 SAMN31871516 femvitroAB5 AMPLICON METAGENOMIC PCR Illumina NovaSeq 6000 SRX18390987 femvitroAB6 SRP410059 PRJNA905798 Genomic DNA extraction was performed by the Nucleospin Tissue XS kit (Macherey-Nagel) after washing tick samples in 1% bleach solution and tick tissue preparation. After quality control, DNA was diluted to 1 ng/ L. To amplicon sequencing of the V3-V4 region of bacterial 16S rRNA was performed by primers 341F (5-CCTAYGGGRBGCASCAG-3) and 806R (5-GGACTACNNGGGTATCTAAT-3). The PCR was performed using Phusion High-Fidelity PCR Master Mix (New England Biolabs) followed by agarose gel electrophoresis. PCR product mixtures were purified using the Qiagen Gel Extraction kit (Qiagen, Germany). Libraries were genereted by NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) and quantified by Qubit and qPCR. Sequencing depth was set as 50k raw reads per sample. SRS15875101 SAMN31871517 femvitroAB6 AMPLICON METAGENOMIC PCR Illumina NovaSeq 6000 SRX18390988 femvivo1 SRP410059 PRJNA905798 Genomic DNA extraction was performed by the Nucleospin Tissue XS kit (Macherey-Nagel) after washing tick samples in 1% bleach solution and tick tissue preparation. After quality control, DNA was diluted to 1 ng/ L. To amplicon sequencing of the V3-V4 region of bacterial 16S rRNA was performed by primers 341F (5-CCTAYGGGRBGCASCAG-3) and 806R (5-GGACTACNNGGGTATCTAAT-3). The PCR was performed using Phusion High-Fidelity PCR Master Mix (New England Biolabs) followed by agarose gel electrophoresis. PCR product mixtures were purified using the Qiagen Gel Extraction kit (Qiagen, Germany). Libraries were genereted by NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) and quantified by Qubit and qPCR. Sequencing depth was set as 50k raw reads per sample. SRS15875102 SAMN31871518 femvivo1 AMPLICON METAGENOMIC PCR Illumina NovaSeq 6000 SRX18390989 femvivo3 SRP410059 PRJNA905798 Genomic DNA extraction was performed by the Nucleospin Tissue XS kit (Macherey-Nagel) after washing tick samples in 1% bleach solution and tick tissue preparation. After quality control, DNA was diluted to 1 ng/ L. To amplicon sequencing of the V3-V4 region of bacterial 16S rRNA was performed by primers 341F (5-CCTAYGGGRBGCASCAG-3) and 806R (5-GGACTACNNGGGTATCTAAT-3). The PCR was performed using Phusion High-Fidelity PCR Master Mix (New England Biolabs) followed by agarose gel electrophoresis. PCR product mixtures were purified using the Qiagen Gel Extraction kit (Qiagen, Germany). Libraries were genereted by NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) and quantified by Qubit and qPCR. Sequencing depth was set as 50k raw reads per sample. SRS15875103 SAMN31871519 femvivo3 AMPLICON METAGENOMIC PCR Illumina NovaSeq 6000 SRX18390990 femvivo4 SRP410059 PRJNA905798 Genomic DNA extraction was performed by the Nucleospin Tissue XS kit (Macherey-Nagel) after washing tick samples in 1% bleach solution and tick tissue preparation. After quality control, DNA was diluted to 1 ng/ L. To amplicon sequencing of the V3-V4 region of bacterial 16S rRNA was performed by primers 341F (5-CCTAYGGGRBGCASCAG-3) and 806R (5-GGACTACNNGGGTATCTAAT-3). The PCR was performed using Phusion High-Fidelity PCR Master Mix (New England Biolabs) followed by agarose gel electrophoresis. PCR product mixtures were purified using the Qiagen Gel Extraction kit (Qiagen, Germany). Libraries were genereted by NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) and quantified by Qubit and qPCR. Sequencing depth was set as 50k raw reads per sample. SRS15875104 SAMN31871520 femvivo4 AMPLICON METAGENOMIC PCR Illumina NovaSeq 6000