SRX18403427 GSM6760168_r1 SRP410292 PRJNA906147 SRS15887262 GSM6760168 GSM6760168 RNA-Seq TRANSCRIPTOMIC cDNA RNAs were prepared with hot phenol extraction method. 400 μg RNA was incubated with MTESA biotin-XX linker (Biotium, BT90066) in the biotinylation buffer (10mM Tris pH7.4-7.5, 1mM EDTA, 400ug RNA, 40ug MTSEA biotin-XX linker) in the dark at 24 °C for 30min with 750 rpm shaking. After biotinylation, Chloroform/Isoamyl alcohol (24:1) was applied to remove excess MTSEA biotin-XX liner. After purification, 200 μL of μMACS streptavidin MicroBeads (Miltenyi Biotec, 130-074-101) was added to bind biotin labelled RNAs and isolate them. The RNAs eluted from streptavidin beads were further cleaned up by RNeasy minElute kit (Qiagen, 74204). To efficiently capture <200 nt fragments from the MinElute spin columns, the amount of ethanol added to the RNA and RLT buffer was increased compared to the manufacturer's recommendation. For a 200 μL sample, 700 μL of RLT buffer and 1,050 μL of 100% ethanol was added, mixed well and applied to the minElute spin columns over three rounds. The remaining protocol was as recommended by Qiagen. The RNA was eluted in 20 μL. Where relevant, in vitro polyadenylation was performed by using E. coli polyA polymerase (EPAP) (NEB, M0276L). Ribosome RNAs were depleted by using RiboMinus Transcriptome Isolation Kit (Thermo Fisher Scientific, 10388792). Library of 3' end sequencing was prepared by using Lexogen QuantSeq 3′ mRNA-Seq Library Prep Kit REV (Lexogen, 16.24) for Illumina and sequenced with single end 75bp reads by NextSeq 500/550 Mid Output Kit v2.5 (150 Cycles) with advanced sequencing science technology platform (STP) at the Francis Crick institute. NextSeq 500 SRX18403428 GSM6760169_r1 SRP410292 PRJNA906147 SRS15887263 GSM6760169 GSM6760169 RNA-Seq TRANSCRIPTOMIC cDNA RNAs were prepared with hot phenol extraction method. 400 μg RNA was incubated with MTESA biotin-XX linker (Biotium, BT90066) in the biotinylation buffer (10mM Tris pH7.4-7.5, 1mM EDTA, 400ug RNA, 40ug MTSEA biotin-XX linker) in the dark at 24 °C for 30min with 750 rpm shaking. After biotinylation, Chloroform/Isoamyl alcohol (24:1) was applied to remove excess MTSEA biotin-XX liner. After purification, 200 μL of μMACS streptavidin MicroBeads (Miltenyi Biotec, 130-074-101) was added to bind biotin labelled RNAs and isolate them. The RNAs eluted from streptavidin beads were further cleaned up by RNeasy minElute kit (Qiagen, 74204). To efficiently capture <200 nt fragments from the MinElute spin columns, the amount of ethanol added to the RNA and RLT buffer was increased compared to the manufacturer's recommendation. For a 200 μL sample, 700 μL of RLT buffer and 1,050 μL of 100% ethanol was added, mixed well and applied to the minElute spin columns over three rounds. The remaining protocol was as recommended by Qiagen. The RNA was eluted in 20 μL. Where relevant, in vitro polyadenylation was performed by using E. coli polyA polymerase (EPAP) (NEB, M0276L). Ribosome RNAs were depleted by using RiboMinus Transcriptome Isolation Kit (Thermo Fisher Scientific, 10388792). Library of 3' end sequencing was prepared by using Lexogen QuantSeq 3′ mRNA-Seq Library Prep Kit REV (Lexogen, 16.24) for Illumina and sequenced with single end 75bp reads by NextSeq 500/550 Mid Output Kit v2.5 (150 Cycles) with advanced sequencing science technology platform (STP) at the Francis Crick institute. NextSeq 500 SRX18403429 GSM6760170_r1 SRP410292 PRJNA906147 SRS15887264 GSM6760170 GSM6760170 RNA-Seq TRANSCRIPTOMIC cDNA RNAs were prepared with hot phenol extraction method. 400 μg RNA was incubated with MTESA biotin-XX linker (Biotium, BT90066) in the biotinylation buffer (10mM Tris pH7.4-7.5, 1mM EDTA, 400ug RNA, 40ug MTSEA biotin-XX linker) in the dark at 24 °C for 30min with 750 rpm shaking. After biotinylation, Chloroform/Isoamyl alcohol (24:1) was applied to remove excess MTSEA biotin-XX liner. After purification, 200 μL of μMACS streptavidin MicroBeads (Miltenyi Biotec, 130-074-101) was added to bind biotin labelled RNAs and isolate them. The RNAs eluted from streptavidin beads were further cleaned up by RNeasy minElute kit (Qiagen, 74204). To efficiently capture <200 nt fragments from the MinElute spin columns, the amount of ethanol added to the RNA and RLT buffer was increased compared to the manufacturer's recommendation. For a 200 μL sample, 700 μL of RLT buffer and 1,050 μL of 100% ethanol was added, mixed well and applied to the minElute spin columns over three rounds. The remaining protocol was as recommended by Qiagen. The RNA was eluted in 20 μL. Where relevant, in vitro polyadenylation was performed by using E. coli polyA polymerase (EPAP) (NEB, M0276L). Ribosome RNAs were depleted by using RiboMinus Transcriptome Isolation Kit (Thermo Fisher Scientific, 10388792). Library of 3' end sequencing was prepared by using Lexogen QuantSeq 3′ mRNA-Seq Library Prep Kit REV (Lexogen, 16.24) for Illumina and sequenced with single end 75bp reads by NextSeq 500/550 Mid Output Kit v2.5 (150 Cycles) with advanced sequencing science technology platform (STP) at the Francis Crick institute. NextSeq 500 SRX18403430 GSM6760171_r1 SRP410292 PRJNA906147 SRS15887265 GSM6760171 GSM6760171 RNA-Seq TRANSCRIPTOMIC cDNA RNAs were prepared with hot phenol extraction method. 400 μg RNA was incubated with MTESA biotin-XX linker (Biotium, BT90066) in the biotinylation buffer (10mM Tris pH7.4-7.5, 1mM EDTA, 400ug RNA, 40ug MTSEA biotin-XX linker) in the dark at 24 °C for 30min with 750 rpm shaking. After biotinylation, Chloroform/Isoamyl alcohol (24:1) was applied to remove excess MTSEA biotin-XX liner. After purification, 200 μL of μMACS streptavidin MicroBeads (Miltenyi Biotec, 130-074-101) was added to bind biotin labelled RNAs and isolate them. The RNAs eluted from streptavidin beads were further cleaned up by RNeasy minElute kit (Qiagen, 74204). To efficiently capture <200 nt fragments from the MinElute spin columns, the amount of ethanol added to the RNA and RLT buffer was increased compared to the manufacturer's recommendation. For a 200 μL sample, 700 μL of RLT buffer and 1,050 μL of 100% ethanol was added, mixed well and applied to the minElute spin columns over three rounds. The remaining protocol was as recommended by Qiagen. The RNA was eluted in 20 μL. Where relevant, in vitro polyadenylation was performed by using E. coli polyA polymerase (EPAP) (NEB, M0276L). Ribosome RNAs were depleted by using RiboMinus Transcriptome Isolation Kit (Thermo Fisher Scientific, 10388792). Library of 3' end sequencing was prepared by using Lexogen QuantSeq 3′ mRNA-Seq Library Prep Kit REV (Lexogen, 16.24) for Illumina and sequenced with single end 75bp reads by NextSeq 500/550 Mid Output Kit v2.5 (150 Cycles) with advanced sequencing science technology platform (STP) at the Francis Crick institute. NextSeq 500 SRX18403431 GSM6760172_r1 SRP410292 PRJNA906147 SRS15887266 GSM6760172 GSM6760172 RNA-Seq TRANSCRIPTOMIC cDNA RNAs were prepared with hot phenol extraction method. 400 μg RNA was incubated with MTESA biotin-XX linker (Biotium, BT90066) in the biotinylation buffer (10mM Tris pH7.4-7.5, 1mM EDTA, 400ug RNA, 40ug MTSEA biotin-XX linker) in the dark at 24 °C for 30min with 750 rpm shaking. After biotinylation, Chloroform/Isoamyl alcohol (24:1) was applied to remove excess MTSEA biotin-XX liner. After purification, 200 μL of μMACS streptavidin MicroBeads (Miltenyi Biotec, 130-074-101) was added to bind biotin labelled RNAs and isolate them. The RNAs eluted from streptavidin beads were further cleaned up by RNeasy minElute kit (Qiagen, 74204). To efficiently capture <200 nt fragments from the MinElute spin columns, the amount of ethanol added to the RNA and RLT buffer was increased compared to the manufacturer's recommendation. For a 200 μL sample, 700 μL of RLT buffer and 1,050 μL of 100% ethanol was added, mixed well and applied to the minElute spin columns over three rounds. The remaining protocol was as recommended by Qiagen. The RNA was eluted in 20 μL. Where relevant, in vitro polyadenylation was performed by using E. coli polyA polymerase (EPAP) (NEB, M0276L). Ribosome RNAs were depleted by using RiboMinus Transcriptome Isolation Kit (Thermo Fisher Scientific, 10388792). Library of 3' end sequencing was prepared by using Lexogen QuantSeq 3′ mRNA-Seq Library Prep Kit REV (Lexogen, 16.24) for Illumina and sequenced with single end 75bp reads by NextSeq 500/550 Mid Output Kit v2.5 (150 Cycles) with advanced sequencing science technology platform (STP) at the Francis Crick institute. NextSeq 500 SRX18403432 GSM6760173_r1 SRP410292 PRJNA906147 SRS15887267 GSM6760173 GSM6760173 RNA-Seq TRANSCRIPTOMIC cDNA RNAs were prepared with hot phenol extraction method. 400 μg RNA was incubated with MTESA biotin-XX linker (Biotium, BT90066) in the biotinylation buffer (10mM Tris pH7.4-7.5, 1mM EDTA, 400ug RNA, 40ug MTSEA biotin-XX linker) in the dark at 24 °C for 30min with 750 rpm shaking. After biotinylation, Chloroform/Isoamyl alcohol (24:1) was applied to remove excess MTSEA biotin-XX liner. After purification, 200 μL of μMACS streptavidin MicroBeads (Miltenyi Biotec, 130-074-101) was added to bind biotin labelled RNAs and isolate them. The RNAs eluted from streptavidin beads were further cleaned up by RNeasy minElute kit (Qiagen, 74204). To efficiently capture <200 nt fragments from the MinElute spin columns, the amount of ethanol added to the RNA and RLT buffer was increased compared to the manufacturer's recommendation. For a 200 μL sample, 700 μL of RLT buffer and 1,050 μL of 100% ethanol was added, mixed well and applied to the minElute spin columns over three rounds. The remaining protocol was as recommended by Qiagen. The RNA was eluted in 20 μL. Where relevant, in vitro polyadenylation was performed by using E. coli polyA polymerase (EPAP) (NEB, M0276L). Ribosome RNAs were depleted by using RiboMinus Transcriptome Isolation Kit (Thermo Fisher Scientific, 10388792). Library of 3' end sequencing was prepared by using Lexogen QuantSeq 3′ mRNA-Seq Library Prep Kit REV (Lexogen, 16.24) for Illumina and sequenced with single end 75bp reads by NextSeq 500/550 Mid Output Kit v2.5 (150 Cycles) with advanced sequencing science technology platform (STP) at the Francis Crick institute. NextSeq 500 SRX18403433 GSM6760174_r1 SRP410292 PRJNA906147 SRS15887268 GSM6760174 GSM6760174 RNA-Seq TRANSCRIPTOMIC cDNA RNAs were prepared with hot phenol extraction method. 400 μg RNA was incubated with MTESA biotin-XX linker (Biotium, BT90066) in the biotinylation buffer (10mM Tris pH7.4-7.5, 1mM EDTA, 400ug RNA, 40ug MTSEA biotin-XX linker) in the dark at 24 °C for 30min with 750 rpm shaking. After biotinylation, Chloroform/Isoamyl alcohol (24:1) was applied to remove excess MTSEA biotin-XX liner. After purification, 200 μL of μMACS streptavidin MicroBeads (Miltenyi Biotec, 130-074-101) was added to bind biotin labelled RNAs and isolate them. The RNAs eluted from streptavidin beads were further cleaned up by RNeasy minElute kit (Qiagen, 74204). To efficiently capture <200 nt fragments from the MinElute spin columns, the amount of ethanol added to the RNA and RLT buffer was increased compared to the manufacturer's recommendation. For a 200 μL sample, 700 μL of RLT buffer and 1,050 μL of 100% ethanol was added, mixed well and applied to the minElute spin columns over three rounds. The remaining protocol was as recommended by Qiagen. The RNA was eluted in 20 μL. Where relevant, in vitro polyadenylation was performed by using E. coli polyA polymerase (EPAP) (NEB, M0276L). Ribosome RNAs were depleted by using RiboMinus Transcriptome Isolation Kit (Thermo Fisher Scientific, 10388792). Library of 3' end sequencing was prepared by using Lexogen QuantSeq 3′ mRNA-Seq Library Prep Kit REV (Lexogen, 16.24) for Illumina and sequenced with single end 75bp reads by NextSeq 500/550 Mid Output Kit v2.5 (150 Cycles) with advanced sequencing science technology platform (STP) at the Francis Crick institute. NextSeq 500 SRX18403434 GSM6760175_r1 SRP410292 PRJNA906147 SRS15887269 GSM6760175 GSM6760175 RNA-Seq TRANSCRIPTOMIC cDNA RNAs were prepared with hot phenol extraction method. 400 μg RNA was incubated with MTESA biotin-XX linker (Biotium, BT90066) in the biotinylation buffer (10mM Tris pH7.4-7.5, 1mM EDTA, 400ug RNA, 40ug MTSEA biotin-XX linker) in the dark at 24 °C for 30min with 750 rpm shaking. After biotinylation, Chloroform/Isoamyl alcohol (24:1) was applied to remove excess MTSEA biotin-XX liner. After purification, 200 μL of μMACS streptavidin MicroBeads (Miltenyi Biotec, 130-074-101) was added to bind biotin labelled RNAs and isolate them. The RNAs eluted from streptavidin beads were further cleaned up by RNeasy minElute kit (Qiagen, 74204). To efficiently capture <200 nt fragments from the MinElute spin columns, the amount of ethanol added to the RNA and RLT buffer was increased compared to the manufacturer's recommendation. For a 200 μL sample, 700 μL of RLT buffer and 1,050 μL of 100% ethanol was added, mixed well and applied to the minElute spin columns over three rounds. The remaining protocol was as recommended by Qiagen. The RNA was eluted in 20 μL. Where relevant, in vitro polyadenylation was performed by using E. coli polyA polymerase (EPAP) (NEB, M0276L). Ribosome RNAs were depleted by using RiboMinus Transcriptome Isolation Kit (Thermo Fisher Scientific, 10388792). Library of 3' end sequencing was prepared by using Lexogen QuantSeq 3′ mRNA-Seq Library Prep Kit REV (Lexogen, 16.24) for Illumina and sequenced with single end 75bp reads by NextSeq 500/550 Mid Output Kit v2.5 (150 Cycles) with advanced sequencing science technology platform (STP) at the Francis Crick institute. NextSeq 500 SRX18403435 GSM6760176_r1 SRP410292 PRJNA906147 SRS15887270 GSM6760176 GSM6760176 RNA-Seq TRANSCRIPTOMIC cDNA RNAs were prepared with hot phenol extraction method. 400 μg RNA was incubated with MTESA biotin-XX linker (Biotium, BT90066) in the biotinylation buffer (10mM Tris pH7.4-7.5, 1mM EDTA, 400ug RNA, 40ug MTSEA biotin-XX linker) in the dark at 24 °C for 30min with 750 rpm shaking. After biotinylation, Chloroform/Isoamyl alcohol (24:1) was applied to remove excess MTSEA biotin-XX liner. After purification, 200 μL of μMACS streptavidin MicroBeads (Miltenyi Biotec, 130-074-101) was added to bind biotin labelled RNAs and isolate them. The RNAs eluted from streptavidin beads were further cleaned up by RNeasy minElute kit (Qiagen, 74204). To efficiently capture <200 nt fragments from the MinElute spin columns, the amount of ethanol added to the RNA and RLT buffer was increased compared to the manufacturer's recommendation. For a 200 μL sample, 700 μL of RLT buffer and 1,050 μL of 100% ethanol was added, mixed well and applied to the minElute spin columns over three rounds. The remaining protocol was as recommended by Qiagen. The RNA was eluted in 20 μL. Where relevant, in vitro polyadenylation was performed by using E. coli polyA polymerase (EPAP) (NEB, M0276L). Ribosome RNAs were depleted by using RiboMinus Transcriptome Isolation Kit (Thermo Fisher Scientific, 10388792). Library of 3' end sequencing was prepared by using Lexogen QuantSeq 3′ mRNA-Seq Library Prep Kit REV (Lexogen, 16.24) for Illumina and sequenced with single end 75bp reads by NextSeq 500/550 Mid Output Kit v2.5 (150 Cycles) with advanced sequencing science technology platform (STP) at the Francis Crick institute. NextSeq 500 SRX18403436 GSM6760177_r1 SRP410292 PRJNA906147 SRS15887271 GSM6760177 GSM6760177 RNA-Seq TRANSCRIPTOMIC cDNA RNAs were prepared with hot phenol extraction method. 400 μg RNA was incubated with MTESA biotin-XX linker (Biotium, BT90066) in the biotinylation buffer (10mM Tris pH7.4-7.5, 1mM EDTA, 400ug RNA, 40ug MTSEA biotin-XX linker) in the dark at 24 °C for 30min with 750 rpm shaking. After biotinylation, Chloroform/Isoamyl alcohol (24:1) was applied to remove excess MTSEA biotin-XX liner. After purification, 200 μL of μMACS streptavidin MicroBeads (Miltenyi Biotec, 130-074-101) was added to bind biotin labelled RNAs and isolate them. The RNAs eluted from streptavidin beads were further cleaned up by RNeasy minElute kit (Qiagen, 74204). To efficiently capture <200 nt fragments from the MinElute spin columns, the amount of ethanol added to the RNA and RLT buffer was increased compared to the manufacturer's recommendation. For a 200 μL sample, 700 μL of RLT buffer and 1,050 μL of 100% ethanol was added, mixed well and applied to the minElute spin columns over three rounds. The remaining protocol was as recommended by Qiagen. The RNA was eluted in 20 μL. Where relevant, in vitro polyadenylation was performed by using E. coli polyA polymerase (EPAP) (NEB, M0276L). Ribosome RNAs were depleted by using RiboMinus Transcriptome Isolation Kit (Thermo Fisher Scientific, 10388792). Library of 3' end sequencing was prepared by using Lexogen QuantSeq 3′ mRNA-Seq Library Prep Kit REV (Lexogen, 16.24) for Illumina and sequenced with single end 75bp reads by NextSeq 500/550 Mid Output Kit v2.5 (150 Cycles) with advanced sequencing science technology platform (STP) at the Francis Crick institute. NextSeq 500 SRX18403437 GSM6760178_r1 SRP410292 PRJNA906147 SRS15887272 GSM6760178 GSM6760178 RNA-Seq TRANSCRIPTOMIC cDNA RNAs were prepared with hot phenol extraction method. 400 μg RNA was incubated with MTESA biotin-XX linker (Biotium, BT90066) in the biotinylation buffer (10mM Tris pH7.4-7.5, 1mM EDTA, 400ug RNA, 40ug MTSEA biotin-XX linker) in the dark at 24 °C for 30min with 750 rpm shaking. After biotinylation, Chloroform/Isoamyl alcohol (24:1) was applied to remove excess MTSEA biotin-XX liner. After purification, 200 μL of μMACS streptavidin MicroBeads (Miltenyi Biotec, 130-074-101) was added to bind biotin labelled RNAs and isolate them. The RNAs eluted from streptavidin beads were further cleaned up by RNeasy minElute kit (Qiagen, 74204). To efficiently capture <200 nt fragments from the MinElute spin columns, the amount of ethanol added to the RNA and RLT buffer was increased compared to the manufacturer's recommendation. For a 200 μL sample, 700 μL of RLT buffer and 1,050 μL of 100% ethanol was added, mixed well and applied to the minElute spin columns over three rounds. The remaining protocol was as recommended by Qiagen. The RNA was eluted in 20 μL. Where relevant, in vitro polyadenylation was performed by using E. coli polyA polymerase (EPAP) (NEB, M0276L). Ribosome RNAs were depleted by using RiboMinus Transcriptome Isolation Kit (Thermo Fisher Scientific, 10388792). Library of 3' end sequencing was prepared by using Lexogen QuantSeq 3′ mRNA-Seq Library Prep Kit REV (Lexogen, 16.24) for Illumina and sequenced with single end 75bp reads by NextSeq 500/550 Mid Output Kit v2.5 (150 Cycles) with advanced sequencing science technology platform (STP) at the Francis Crick institute. NextSeq 500 SRX18403438 GSM6760179_r1 SRP410292 PRJNA906147 SRS15887273 GSM6760179 GSM6760179 RNA-Seq TRANSCRIPTOMIC cDNA RNAs were prepared with hot phenol extraction method. 400 μg RNA was incubated with MTESA biotin-XX linker (Biotium, BT90066) in the biotinylation buffer (10mM Tris pH7.4-7.5, 1mM EDTA, 400ug RNA, 40ug MTSEA biotin-XX linker) in the dark at 24 °C for 30min with 750 rpm shaking. After biotinylation, Chloroform/Isoamyl alcohol (24:1) was applied to remove excess MTSEA biotin-XX liner. After purification, 200 μL of μMACS streptavidin MicroBeads (Miltenyi Biotec, 130-074-101) was added to bind biotin labelled RNAs and isolate them. The RNAs eluted from streptavidin beads were further cleaned up by RNeasy minElute kit (Qiagen, 74204). To efficiently capture <200 nt fragments from the MinElute spin columns, the amount of ethanol added to the RNA and RLT buffer was increased compared to the manufacturer's recommendation. For a 200 μL sample, 700 μL of RLT buffer and 1,050 μL of 100% ethanol was added, mixed well and applied to the minElute spin columns over three rounds. The remaining protocol was as recommended by Qiagen. The RNA was eluted in 20 μL. Where relevant, in vitro polyadenylation was performed by using E. coli polyA polymerase (EPAP) (NEB, M0276L). Ribosome RNAs were depleted by using RiboMinus Transcriptome Isolation Kit (Thermo Fisher Scientific, 10388792). Library of 3' end sequencing was prepared by using Lexogen QuantSeq 3′ mRNA-Seq Library Prep Kit REV (Lexogen, 16.24) for Illumina and sequenced with single end 75bp reads by NextSeq 500/550 Mid Output Kit v2.5 (150 Cycles) with advanced sequencing science technology platform (STP) at the Francis Crick institute. NextSeq 500