SRX18412618 GSM6761427_r1 SRP410426 PRJNA906504 SRS15895635 GSM6761427 GSM6761427 RNA-Seq TRANSCRIPTOMIC cDNA Live CD4+ T cells were sorted on a FACS Aria cell sorter and collected into eppendorfs. Cells were lysed in 100uL extraction buffer using PicoPure RNA extraction kit according to manufacturer's instructions. An on column DNase digest was performed. Libraries were constructed using Lexogen Quantseq 3' mRNA Forward kits according to the manufacturer's instructions using a starting amount of 5ng of RNA and UMIs 3' mRNA-seq FWD UMI NextSeq 500 SRX18412619 GSM6761428_r1 SRP410426 PRJNA906504 SRS15895636 GSM6761428 GSM6761428 RNA-Seq TRANSCRIPTOMIC cDNA Live CD4+ T cells were sorted on a FACS Aria cell sorter and collected into eppendorfs. Cells were lysed in 100uL extraction buffer using PicoPure RNA extraction kit according to manufacturer's instructions. An on column DNase digest was performed. Libraries were constructed using Lexogen Quantseq 3' mRNA Forward kits according to the manufacturer's instructions using a starting amount of 5ng of RNA and UMIs 3' mRNA-seq FWD UMI NextSeq 500 SRX18412620 GSM6761429_r1 SRP410426 PRJNA906504 SRS15895637 GSM6761429 GSM6761429 RNA-Seq TRANSCRIPTOMIC cDNA Live CD4+ T cells were sorted on a FACS Aria cell sorter and collected into eppendorfs. Cells were lysed in 100uL extraction buffer using PicoPure RNA extraction kit according to manufacturer's instructions. An on column DNase digest was performed. Libraries were constructed using Lexogen Quantseq 3' mRNA Forward kits according to the manufacturer's instructions using a starting amount of 5ng of RNA and UMIs 3' mRNA-seq FWD UMI NextSeq 500 SRX18412621 GSM6761430_r1 SRP410426 PRJNA906504 SRS15895638 GSM6761430 GSM6761430 RNA-Seq TRANSCRIPTOMIC cDNA Live CD4+ T cells were sorted on a FACS Aria cell sorter and collected into eppendorfs. Cells were lysed in 100uL extraction buffer using PicoPure RNA extraction kit according to manufacturer's instructions. An on column DNase digest was performed. Libraries were constructed using Lexogen Quantseq 3' mRNA Forward kits according to the manufacturer's instructions using a starting amount of 5ng of RNA and UMIs 3' mRNA-seq FWD UMI NextSeq 500 SRX18412622 GSM6761431_r1 SRP410426 PRJNA906504 SRS15895639 GSM6761431 GSM6761431 RNA-Seq TRANSCRIPTOMIC cDNA Live CD4+ T cells were sorted on a FACS Aria cell sorter and collected into eppendorfs. Cells were lysed in 100uL extraction buffer using PicoPure RNA extraction kit according to manufacturer's instructions. An on column DNase digest was performed. Libraries were constructed using Lexogen Quantseq 3' mRNA Forward kits according to the manufacturer's instructions using a starting amount of 5ng of RNA and UMIs 3' mRNA-seq FWD UMI NextSeq 500 SRX18412623 GSM6761432_r1 SRP410426 PRJNA906504 SRS15895642 GSM6761432 GSM6761432 RNA-Seq TRANSCRIPTOMIC cDNA Live CD4+ T cells were sorted on a FACS Aria cell sorter and collected into eppendorfs. Cells were lysed in 100uL extraction buffer using PicoPure RNA extraction kit according to manufacturer's instructions. An on column DNase digest was performed. Libraries were constructed using Lexogen Quantseq 3' mRNA Forward kits according to the manufacturer's instructions using a starting amount of 5ng of RNA and UMIs 3' mRNA-seq FWD UMI NextSeq 500 SRX18412624 GSM6761433_r1 SRP410426 PRJNA906504 SRS15895640 GSM6761433 GSM6761433 RNA-Seq TRANSCRIPTOMIC cDNA Live CD4+ T cells were sorted on a FACS Aria cell sorter and collected into eppendorfs. Cells were lysed in 100uL extraction buffer using PicoPure RNA extraction kit according to manufacturer's instructions. An on column DNase digest was performed. Libraries were constructed using Lexogen Quantseq 3' mRNA Forward kits according to the manufacturer's instructions using a starting amount of 5ng of RNA and UMIs 3' mRNA-seq FWD UMI NextSeq 500 SRX18412625 GSM6761434_r1 SRP410426 PRJNA906504 SRS15895641 GSM6761434 GSM6761434 RNA-Seq TRANSCRIPTOMIC cDNA Live CD4+ T cells were sorted on a FACS Aria cell sorter and collected into eppendorfs. Cells were lysed in 100uL extraction buffer using PicoPure RNA extraction kit according to manufacturer's instructions. An on column DNase digest was performed. Libraries were constructed using Lexogen Quantseq 3' mRNA Forward kits according to the manufacturer's instructions using a starting amount of 5ng of RNA and UMIs 3' mRNA-seq FWD UMI NextSeq 500 SRX18412626 GSM6761435_r1 SRP410426 PRJNA906504 SRS15895643 GSM6761435 GSM6761435 RNA-Seq TRANSCRIPTOMIC cDNA Live CD4+ T cells were sorted on a FACS Aria cell sorter and collected into eppendorfs. Cells were lysed in 100uL extraction buffer using PicoPure RNA extraction kit according to manufacturer's instructions. An on column DNase digest was performed. Libraries were constructed using Lexogen Quantseq 3' mRNA Forward kits according to the manufacturer's instructions using a starting amount of 5ng of RNA and UMIs 3' mRNA-seq FWD UMI NextSeq 500 SRX18412627 GSM6761436_r1 SRP410426 PRJNA906504 SRS15895644 GSM6761436 GSM6761436 RNA-Seq TRANSCRIPTOMIC cDNA Live CD4+ T cells were sorted on a FACS Aria cell sorter and collected into eppendorfs. Cells were lysed in 100uL extraction buffer using PicoPure RNA extraction kit according to manufacturer's instructions. An on column DNase digest was performed. Libraries were constructed using Lexogen Quantseq 3' mRNA Forward kits according to the manufacturer's instructions using a starting amount of 5ng of RNA and UMIs 3' mRNA-seq FWD UMI NextSeq 500 SRX18412628 GSM6761437_r1 SRP410426 PRJNA906504 SRS15895645 GSM6761437 GSM6761437 RNA-Seq TRANSCRIPTOMIC cDNA Live CD4+ T cells were sorted on a FACS Aria cell sorter and collected into eppendorfs. Cells were lysed in 100uL extraction buffer using PicoPure RNA extraction kit according to manufacturer's instructions. An on column DNase digest was performed. Libraries were constructed using Lexogen Quantseq 3' mRNA Forward kits according to the manufacturer's instructions using a starting amount of 5ng of RNA and UMIs 3' mRNA-seq FWD UMI NextSeq 500 SRX18412629 GSM6761438_r1 SRP410426 PRJNA906504 SRS15895646 GSM6761438 GSM6761438 RNA-Seq TRANSCRIPTOMIC cDNA Live CD4+ T cells were sorted on a FACS Aria cell sorter and collected into eppendorfs. Cells were lysed in 100uL extraction buffer using PicoPure RNA extraction kit according to manufacturer's instructions. An on column DNase digest was performed. Libraries were constructed using Lexogen Quantseq 3' mRNA Forward kits according to the manufacturer's instructions using a starting amount of 5ng of RNA and UMIs 3' mRNA-seq FWD UMI NextSeq 500