SRX18413465 GSM6761960_r1 SRP410447 PRJNA906525 SRS15896432 GSM6761960 GSM6761960 ATAC-seq GENOMIC other 5.0 × 104 cells were lysed in ice-cold ATAC-Resuspension Buffer (RSB) containing 0.1% NP40, 0.1% Tween-20 and 0.01% digitonin at 4 °C for 3 min, then washed with ATAC-RSB containing only 0.1% Tween-20. Nuclei were spun down by centrifugation and incubated in 50 mL of transposition buffer (25 mL 2×TD buffer, 16.5 mL PBS, 0.5 mL 1% digitonin, 0.5 mL 10% Tween-20, 5 mL H2O, 2.5 mL TDE1 Tagment DNA Enzyme (Illumina Tagment DNA TDE1 Enzyme and Buffer Kit, Illumina)) at 37 °C for 30 min in a thermomixer with 1000 rpm mixing. Transposed DNA was purified with a DNA Clean & Concentrator Kit (Zymo Research). Libraries were prepared with a Nextera DNA Library Prep Kit. Illumina NovaSeq 6000 SRX18413466 GSM6761961_r1 SRP410447 PRJNA906525 SRS15896433 GSM6761961 GSM6761961 ATAC-seq GENOMIC other 5.0 × 104 cells were lysed in ice-cold ATAC-Resuspension Buffer (RSB) containing 0.1% NP40, 0.1% Tween-20 and 0.01% digitonin at 4 °C for 3 min, then washed with ATAC-RSB containing only 0.1% Tween-20. Nuclei were spun down by centrifugation and incubated in 50 mL of transposition buffer (25 mL 2×TD buffer, 16.5 mL PBS, 0.5 mL 1% digitonin, 0.5 mL 10% Tween-20, 5 mL H2O, 2.5 mL TDE1 Tagment DNA Enzyme (Illumina Tagment DNA TDE1 Enzyme and Buffer Kit, Illumina)) at 37 °C for 30 min in a thermomixer with 1000 rpm mixing. Transposed DNA was purified with a DNA Clean & Concentrator Kit (Zymo Research). Libraries were prepared with a Nextera DNA Library Prep Kit. Illumina NovaSeq 6000 SRX18413467 GSM6761962_r1 SRP410447 PRJNA906525 SRS15896434 GSM6761962 GSM6761962 ATAC-seq GENOMIC other 5.0 × 104 cells were lysed in ice-cold ATAC-Resuspension Buffer (RSB) containing 0.1% NP40, 0.1% Tween-20 and 0.01% digitonin at 4 °C for 3 min, then washed with ATAC-RSB containing only 0.1% Tween-20. Nuclei were spun down by centrifugation and incubated in 50 mL of transposition buffer (25 mL 2×TD buffer, 16.5 mL PBS, 0.5 mL 1% digitonin, 0.5 mL 10% Tween-20, 5 mL H2O, 2.5 mL TDE1 Tagment DNA Enzyme (Illumina Tagment DNA TDE1 Enzyme and Buffer Kit, Illumina)) at 37 °C for 30 min in a thermomixer with 1000 rpm mixing. Transposed DNA was purified with a DNA Clean & Concentrator Kit (Zymo Research). Libraries were prepared with a Nextera DNA Library Prep Kit. Illumina NovaSeq 6000 SRX18413468 GSM6761963_r1 SRP410447 PRJNA906525 SRS15896435 GSM6761963 GSM6761963 ATAC-seq GENOMIC other 5.0 × 104 cells were lysed in ice-cold ATAC-Resuspension Buffer (RSB) containing 0.1% NP40, 0.1% Tween-20 and 0.01% digitonin at 4 °C for 3 min, then washed with ATAC-RSB containing only 0.1% Tween-20. Nuclei were spun down by centrifugation and incubated in 50 mL of transposition buffer (25 mL 2×TD buffer, 16.5 mL PBS, 0.5 mL 1% digitonin, 0.5 mL 10% Tween-20, 5 mL H2O, 2.5 mL TDE1 Tagment DNA Enzyme (Illumina Tagment DNA TDE1 Enzyme and Buffer Kit, Illumina)) at 37 °C for 30 min in a thermomixer with 1000 rpm mixing. Transposed DNA was purified with a DNA Clean & Concentrator Kit (Zymo Research). Libraries were prepared with a Nextera DNA Library Prep Kit. Illumina NovaSeq 6000 SRX18413469 GSM6761964_r1 SRP410447 PRJNA906525 SRS15896436 GSM6761964 GSM6761964 ATAC-seq GENOMIC other 5.0 × 104 cells were lysed in ice-cold ATAC-Resuspension Buffer (RSB) containing 0.1% NP40, 0.1% Tween-20 and 0.01% digitonin at 4 °C for 3 min, then washed with ATAC-RSB containing only 0.1% Tween-20. Nuclei were spun down by centrifugation and incubated in 50 mL of transposition buffer (25 mL 2×TD buffer, 16.5 mL PBS, 0.5 mL 1% digitonin, 0.5 mL 10% Tween-20, 5 mL H2O, 2.5 mL TDE1 Tagment DNA Enzyme (Illumina Tagment DNA TDE1 Enzyme and Buffer Kit, Illumina)) at 37 °C for 30 min in a thermomixer with 1000 rpm mixing. Transposed DNA was purified with a DNA Clean & Concentrator Kit (Zymo Research). Libraries were prepared with a Nextera DNA Library Prep Kit. Illumina NovaSeq 6000 SRX18413470 GSM6761965_r1 SRP410447 PRJNA906525 SRS15896437 GSM6761965 GSM6761965 ATAC-seq GENOMIC other 5.0 × 104 cells were lysed in ice-cold ATAC-Resuspension Buffer (RSB) containing 0.1% NP40, 0.1% Tween-20 and 0.01% digitonin at 4 °C for 3 min, then washed with ATAC-RSB containing only 0.1% Tween-20. Nuclei were spun down by centrifugation and incubated in 50 mL of transposition buffer (25 mL 2×TD buffer, 16.5 mL PBS, 0.5 mL 1% digitonin, 0.5 mL 10% Tween-20, 5 mL H2O, 2.5 mL TDE1 Tagment DNA Enzyme (Illumina Tagment DNA TDE1 Enzyme and Buffer Kit, Illumina)) at 37 °C for 30 min in a thermomixer with 1000 rpm mixing. Transposed DNA was purified with a DNA Clean & Concentrator Kit (Zymo Research). Libraries were prepared with a Nextera DNA Library Prep Kit. Illumina NovaSeq 6000