SRX13328628 GSM5719427_r1 SRP349449 PRJNA786607 SRS11234675 GSM5719427 GSM5719427 OTHER GENOMIC other 500 cells for each sample were fixed by 1% w/v formaldehyde solution and incubating at RT for 10 minutes. The fixed cells were washed with 1 ml PBS, then, 1 ml RIPA buffer was added to each sample, and incubated at RT for 5 minutes with rotation. Cell pellets were rinsed with 500 μl binding buffer twice and resuspended in 50 μl binding buffer. To prepare anti-TCF-1-bound protein A–micrococcal nuclease (pA-MNase; antibody + PA-MNase), anti-TCF-1 and the PA-MNase at a molecular ratio of 1:2 were pre-incubated at 4 °C for 30 minutes in 50 μl binding buffer. TCF-1 + PA-MNase were added to 50 μl binding buffer-resuspended cells and incubated for 1 hour at 4 °C with rotation. Cells were washed using 200 μl wash buffer three times and pelleted by centrifugation at 600g for 2 minutes. Next, cells were rinsed using 200 μl rinsing buffer. The MNase digestion was initiated by resuspending the rinsed cells in 40 μl reaction solution buffer and incubatingat 37 °C for 3 minutes. The reaction was stopped by adding 80 μl stop buffer and 1 μl proteinase K (Sigma–Aldrich), then incubating at 65 °C overnight. DNA was purified using phenol–chloroformextraction and ethanol precipitation. the ChIC-seq libraries were prepared through as previously described (PMID: 31358996). Illumina NovaSeq 6000 SRX13328629 GSM5719428_r1 SRP349449 PRJNA786607 SRS11234676 GSM5719428 GSM5719428 OTHER GENOMIC other 500 cells for each sample were fixed by 1% w/v formaldehyde solution and incubating at RT for 10 minutes. The fixed cells were washed with 1 ml PBS, then, 1 ml RIPA buffer was added to each sample, and incubated at RT for 5 minutes with rotation. Cell pellets were rinsed with 500 μl binding buffer twice and resuspended in 50 μl binding buffer. To prepare anti-TCF-1-bound protein A–micrococcal nuclease (pA-MNase; antibody + PA-MNase), anti-TCF-1 and the PA-MNase at a molecular ratio of 1:2 were pre-incubated at 4 °C for 30 minutes in 50 μl binding buffer. TCF-1 + PA-MNase were added to 50 μl binding buffer-resuspended cells and incubated for 1 hour at 4 °C with rotation. Cells were washed using 200 μl wash buffer three times and pelleted by centrifugation at 600g for 2 minutes. Next, cells were rinsed using 200 μl rinsing buffer. The MNase digestion was initiated by resuspending the rinsed cells in 40 μl reaction solution buffer and incubatingat 37 °C for 3 minutes. The reaction was stopped by adding 80 μl stop buffer and 1 μl proteinase K (Sigma–Aldrich), then incubating at 65 °C overnight. DNA was purified using phenol–chloroformextraction and ethanol precipitation. the ChIC-seq libraries were prepared through as previously described (PMID: 31358996). Illumina NovaSeq 6000