SRX18424810 bRNA-1 SRP410523 bp0 RNA was collected from O4-microbead-purified cells using RNeasy Micro Kit (Qiagen 74004) following the manufacturers instructions. cDNA was prepared using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490) and xGen Broad-range RNA Library Prep (IDT, 1009813) with xGen Normalase UDI Primers (IDT). This pool was subjected to 151bp paired-end sequencing according to the manufacturers protocol (Illumina NovaSeq). BCL Convert Conversion Software v3.9.3 (Illumina) was used to generate de-multiplexed Fastq files. SRS15907598 5434-TK-1 bRNA-1 RNA-Seq TRANSCRIPTOMIC cDNA Illumina NovaSeq 6000 SRX18424811 bRNA-2 SRP410523 bp0 RNA was collected from O4-microbead-purified cells using RNeasy Micro Kit (Qiagen 74004) following the manufacturers instructions. cDNA was prepared using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490) and xGen Broad-range RNA Library Prep (IDT, 1009813) with xGen Normalase UDI Primers (IDT). This pool was subjected to 151bp paired-end sequencing according to the manufacturers protocol (Illumina NovaSeq). BCL Convert Conversion Software v3.9.3 (Illumina) was used to generate de-multiplexed Fastq files. SRS15907599 5434-TK-2 bRNA-2 RNA-Seq TRANSCRIPTOMIC cDNA Illumina NovaSeq 6000 SRX18424812 snRNA-5 SRP410523 bp0 Brains were collected from P16 mice and the forebrain was isolated and flash frozen in liquid nitrogen. Nuclei were isolated using a protocol adapted from Habib et al. (89). Forebrains were homogenized using a glass Dounce tissue grinder (25X with pestle A and 25X with pestle B) in 2 mL of EZ PREP buffer (Sigma NUC-101). Homogenized tissues were incubated on ice for 5 minutes with an additional 2 mL of EZ PREP. Nuclei were pelleted by centrifuging at 500g for 5 minutes at 4C. Nuclei were washed with 4 mL EZ PREP and incubated on ice for an additional 5 minutes. Nuclei were centrifuged again and washed in 4 mL of nuclear suspension buffer (0.01% BSA, RNase inhibitor [Takara 2313A]) followed by centrifugation for 5 minutes at 500g. The nuclear pellet was resuspended in 1 mL of nuclear suspension buffer before filtering through a 35 M cell strainer (Corning 352235) and counted. Nuclei were diluted to a final concentration of 800-1500 cell/L. Single-nuclear libraries were generated using the 10X Chromium Controller system. Libraries were sequenced using an Illumina NovaSeq 6000. SRS15907600 2764-TK-3 snRNA-5 OTHER OTHER cDNA Illumina NovaSeq 6000 SRX18424813 snRNA-6 SRP410523 bp0 Brains were collected from P16 mice and the forebrain was isolated and flash frozen in liquid nitrogen. Nuclei were isolated using a protocol adapted from Habib et al. (89). Forebrains were homogenized using a glass Dounce tissue grinder (25X with pestle A and 25X with pestle B) in 2 mL of EZ PREP buffer (Sigma NUC-101). Homogenized tissues were incubated on ice for 5 minutes with an additional 2 mL of EZ PREP. Nuclei were pelleted by centrifuging at 500g for 5 minutes at 4C. Nuclei were washed with 4 mL EZ PREP and incubated on ice for an additional 5 minutes. Nuclei were centrifuged again and washed in 4 mL of nuclear suspension buffer (0.01% BSA, RNase inhibitor [Takara 2313A]) followed by centrifugation for 5 minutes at 500g. The nuclear pellet was resuspended in 1 mL of nuclear suspension buffer before filtering through a 35 M cell strainer (Corning 352235) and counted. Nuclei were diluted to a final concentration of 800-1500 cell/L. Single-nuclear libraries were generated using the 10X Chromium Controller system. Libraries were sequenced using an Illumina NovaSeq 6000. SRS15907601 2764-TK-4 snRNA-6 OTHER OTHER cDNA Illumina NovaSeq 6000 SRX18424814 bRNA-3 SRP410523 bp0 RNA was collected from O4-microbead-purified cells using RNeasy Micro Kit (Qiagen 74004) following the manufacturers instructions. cDNA was prepared using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490) and xGen Broad-range RNA Library Prep (IDT, 1009813) with xGen Normalase UDI Primers (IDT). This pool was subjected to 151bp paired-end sequencing according to the manufacturers protocol (Illumina NovaSeq). BCL Convert Conversion Software v3.9.3 (Illumina) was used to generate de-multiplexed Fastq files. SRS15907603 5434-TK-3 bRNA-3 RNA-Seq TRANSCRIPTOMIC cDNA Illumina NovaSeq 6000 SRX18424815 bRNA-4 SRP410523 bp0 RNA was collected from O4-microbead-purified cells using RNeasy Micro Kit (Qiagen 74004) following the manufacturers instructions. cDNA was prepared using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490) and xGen Broad-range RNA Library Prep (IDT, 1009813) with xGen Normalase UDI Primers (IDT). This pool was subjected to 151bp paired-end sequencing according to the manufacturers protocol (Illumina NovaSeq). BCL Convert Conversion Software v3.9.3 (Illumina) was used to generate de-multiplexed Fastq files. SRS15907602 5434-TK-4 bRNA-4 RNA-Seq TRANSCRIPTOMIC cDNA Illumina NovaSeq 6000 SRX18424816 bRNA-5 SRP410523 bp0 RNA was collected from O4-microbead-purified cells using RNeasy Micro Kit (Qiagen 74004) following the manufacturers instructions. cDNA was prepared using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490) and xGen Broad-range RNA Library Prep (IDT, 1009813) with xGen Normalase UDI Primers (IDT). This pool was subjected to 151bp paired-end sequencing according to the manufacturers protocol (Illumina NovaSeq). BCL Convert Conversion Software v3.9.3 (Illumina) was used to generate de-multiplexed Fastq files. SRS15907605 5434-TK-5 bRNA-5 RNA-Seq TRANSCRIPTOMIC cDNA Illumina NovaSeq 6000 SRX18424817 bRNA-6 SRP410523 bp0 RNA was collected from O4-microbead-purified cells using RNeasy Micro Kit (Qiagen 74004) following the manufacturers instructions. cDNA was prepared using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490) and xGen Broad-range RNA Library Prep (IDT, 1009813) with xGen Normalase UDI Primers (IDT). This pool was subjected to 151bp paired-end sequencing according to the manufacturers protocol (Illumina NovaSeq). BCL Convert Conversion Software v3.9.3 (Illumina) was used to generate de-multiplexed Fastq files. SRS15907604 5434-TK-6 bRNA-6 RNA-Seq TRANSCRIPTOMIC cDNA Illumina NovaSeq 6000 SRX18424818 snRNA-1 SRP410523 bp0 Brains were collected from P16 mice and the forebrain was isolated and flash frozen in liquid nitrogen. Nuclei were isolated using a protocol adapted from Habib et al. (89). Forebrains were homogenized using a glass Dounce tissue grinder (25X with pestle A and 25X with pestle B) in 2 mL of EZ PREP buffer (Sigma NUC-101). Homogenized tissues were incubated on ice for 5 minutes with an additional 2 mL of EZ PREP. Nuclei were pelleted by centrifuging at 500g for 5 minutes at 4C. Nuclei were washed with 4 mL EZ PREP and incubated on ice for an additional 5 minutes. Nuclei were centrifuged again and washed in 4 mL of nuclear suspension buffer (0.01% BSA, RNase inhibitor [Takara 2313A]) followed by centrifugation for 5 minutes at 500g. The nuclear pellet was resuspended in 1 mL of nuclear suspension buffer before filtering through a 35 M cell strainer (Corning 352235) and counted. Nuclei were diluted to a final concentration of 800-1500 cell/L. Single-nuclear libraries were generated using the 10X Chromium Controller system. Libraries were sequenced using an Illumina NovaSeq 6000. SRS15907607 2339-TK-1 snRNA-1 OTHER OTHER cDNA Illumina NovaSeq 6000 SRX18424819 snRNA-2 SRP410523 bp0 Brains were collected from P16 mice and the forebrain was isolated and flash frozen in liquid nitrogen. Nuclei were isolated using a protocol adapted from Habib et al. (89). Forebrains were homogenized using a glass Dounce tissue grinder (25X with pestle A and 25X with pestle B) in 2 mL of EZ PREP buffer (Sigma NUC-101). Homogenized tissues were incubated on ice for 5 minutes with an additional 2 mL of EZ PREP. Nuclei were pelleted by centrifuging at 500g for 5 minutes at 4C. Nuclei were washed with 4 mL EZ PREP and incubated on ice for an additional 5 minutes. Nuclei were centrifuged again and washed in 4 mL of nuclear suspension buffer (0.01% BSA, RNase inhibitor [Takara 2313A]) followed by centrifugation for 5 minutes at 500g. The nuclear pellet was resuspended in 1 mL of nuclear suspension buffer before filtering through a 35 M cell strainer (Corning 352235) and counted. Nuclei were diluted to a final concentration of 800-1500 cell/L. Single-nuclear libraries were generated using the 10X Chromium Controller system. Libraries were sequenced using an Illumina NovaSeq 6000. SRS15907606 2339-TK-2 snRNA-2 OTHER OTHER cDNA Illumina NovaSeq 6000 SRX18424820 snRNA-3 SRP410523 bp0 Brains were collected from P16 mice and the forebrain was isolated and flash frozen in liquid nitrogen. Nuclei were isolated using a protocol adapted from Habib et al. (89). Forebrains were homogenized using a glass Dounce tissue grinder (25X with pestle A and 25X with pestle B) in 2 mL of EZ PREP buffer (Sigma NUC-101). Homogenized tissues were incubated on ice for 5 minutes with an additional 2 mL of EZ PREP. Nuclei were pelleted by centrifuging at 500g for 5 minutes at 4C. Nuclei were washed with 4 mL EZ PREP and incubated on ice for an additional 5 minutes. Nuclei were centrifuged again and washed in 4 mL of nuclear suspension buffer (0.01% BSA, RNase inhibitor [Takara 2313A]) followed by centrifugation for 5 minutes at 500g. The nuclear pellet was resuspended in 1 mL of nuclear suspension buffer before filtering through a 35 M cell strainer (Corning 352235) and counted. Nuclei were diluted to a final concentration of 800-1500 cell/L. Single-nuclear libraries were generated using the 10X Chromium Controller system. Libraries were sequenced using an Illumina NovaSeq 6000. SRS15907608 2764-TK-1 snRNA-3 OTHER OTHER cDNA Illumina NovaSeq 6000 SRX18424821 snRNA-4 SRP410523 bp0 Brains were collected from P16 mice and the forebrain was isolated and flash frozen in liquid nitrogen. Nuclei were isolated using a protocol adapted from Habib et al. (89). Forebrains were homogenized using a glass Dounce tissue grinder (25X with pestle A and 25X with pestle B) in 2 mL of EZ PREP buffer (Sigma NUC-101). Homogenized tissues were incubated on ice for 5 minutes with an additional 2 mL of EZ PREP. Nuclei were pelleted by centrifuging at 500g for 5 minutes at 4C. Nuclei were washed with 4 mL EZ PREP and incubated on ice for an additional 5 minutes. Nuclei were centrifuged again and washed in 4 mL of nuclear suspension buffer (0.01% BSA, RNase inhibitor [Takara 2313A]) followed by centrifugation for 5 minutes at 500g. The nuclear pellet was resuspended in 1 mL of nuclear suspension buffer before filtering through a 35 M cell strainer (Corning 352235) and counted. Nuclei were diluted to a final concentration of 800-1500 cell/L. Single-nuclear libraries were generated using the 10X Chromium Controller system. Libraries were sequenced using an Illumina NovaSeq 6000. SRS15907609 2764-TK-2 snRNA-4 OTHER OTHER cDNA Illumina NovaSeq 6000