SRP410529 PRJNA906701 GSE219048 Transcription Analysis of yZSJ025 We carried out RNA-seq to compare gene expression and recombination frequency of yZSJ025. The transcriptome data allowed us to analyze the expression levels and the rearrangement frequencies at different loci across the synthetic chromosomes. Overall design: PCRTags were used to distinguish the transcription of wild-type genes from the transcription of synthetic genes. GSE219048 pubmed 37223244