SRP410653 PRJNA906497 Nasal fungal ITS amplicons obtained from healthy and BRD-positive pen-mates Holstein steers Bovine respiratory disease (BRD) is an ongoing and vital problem in dairy and beef cattle. Multiple studies have looked into the bacterial and viral community related to BRD development. However, no studies have investigated the fungal community between healthy and BRD-affected cattle. Thus, the study's objective focuses on the characterization of the nasal mycobiome of apparently healthy (n=73) and BRD-affected (n=56) pen-mates Holstein steers. For the nasal mycobiome analysis, the Internal transcribed spacer (ITS) was amplified and sequenced using Illumina MiSeq Sequencer.