SRX18439004 GSM6771414_r1 SRP410703 PRJNA907026 SRS15920737 GSM6771414 GSM6771414 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000 SRX18439005 GSM6771415_r1 SRP410703 PRJNA907026 SRS15920740 GSM6771415 GSM6771415 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000 SRX18439006 GSM6771416_r1 SRP410703 PRJNA907026 SRS15920738 GSM6771416 GSM6771416 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000 SRX18439007 GSM6771417_r1 SRP410703 PRJNA907026 SRS15920739 GSM6771417 GSM6771417 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000 SRX18439008 GSM6771418_r1 SRP410703 PRJNA907026 SRS15920741 GSM6771418 GSM6771418 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000 SRX18439009 GSM6771419_r1 SRP410703 PRJNA907026 SRS15920742 GSM6771419 GSM6771419 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000 SRX18439010 GSM6771420_r1 SRP410703 PRJNA907026 SRS15920743 GSM6771420 GSM6771420 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000 SRX18439011 GSM6771421_r1 SRP410703 PRJNA907026 SRS15920744 GSM6771421 GSM6771421 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000 SRX18439012 GSM6771422_r1 SRP410703 PRJNA907026 SRS15920745 GSM6771422 GSM6771422 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000 SRX18439013 GSM6771423_r1 SRP410703 PRJNA907026 SRS15920746 GSM6771423 GSM6771423 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000 SRX18439014 GSM6771424_r1 SRP410703 PRJNA907026 SRS15920747 GSM6771424 GSM6771424 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000 SRX18439015 GSM6771425_r1 SRP410703 PRJNA907026 SRS15920748 GSM6771425 GSM6771425 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000 SRX18439016 GSM6771426_r1 SRP410703 PRJNA907026 SRS15920749 GSM6771426 GSM6771426 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000 SRX18439017 GSM6771427_r1 SRP410703 PRJNA907026 SRS15920750 GSM6771427 GSM6771427 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000 SRX18439018 GSM6771428_r1 SRP410703 PRJNA907026 SRS15920751 GSM6771428 GSM6771428 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000 SRX18439019 GSM6771429_r1 SRP410703 PRJNA907026 SRS15920752 GSM6771429 GSM6771429 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000 SRX18439020 GSM6771430_r1 SRP410703 PRJNA907026 SRS15920753 GSM6771430 GSM6771430 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000 SRX18439021 GSM6771431_r1 SRP410703 PRJNA907026 SRS15920754 GSM6771431 GSM6771431 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000 SRX18439022 GSM6771448_r1 SRP410703 PRJNA907026 SRS15920755 GSM6771448 GSM6771448 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000 SRX18439023 GSM6771449_r1 SRP410703 PRJNA907026 SRS15920756 GSM6771449 GSM6771449 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000 SRX18439024 GSM6771432_r1 SRP410703 PRJNA907026 SRS15920757 GSM6771432 GSM6771432 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000 SRX18439025 GSM6771433_r1 SRP410703 PRJNA907026 SRS15920758 GSM6771433 GSM6771433 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000 SRX18439026 GSM6771434_r1 SRP410703 PRJNA907026 SRS15920759 GSM6771434 GSM6771434 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000 SRX18439027 GSM6771435_r1 SRP410703 PRJNA907026 SRS15920760 GSM6771435 GSM6771435 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000 SRX18439028 GSM6771436_r1 SRP410703 PRJNA907026 SRS15920762 GSM6771436 GSM6771436 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000 SRX18439029 GSM6771437_r1 SRP410703 PRJNA907026 SRS15920761 GSM6771437 GSM6771437 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000 SRX18439030 GSM6771438_r1 SRP410703 PRJNA907026 SRS15920763 GSM6771438 GSM6771438 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000 SRX18439031 GSM6771439_r1 SRP410703 PRJNA907026 SRS15920764 GSM6771439 GSM6771439 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000 SRX18439032 GSM6771440_r1 SRP410703 PRJNA907026 SRS15920765 GSM6771440 GSM6771440 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000 SRX18439033 GSM6771441_r1 SRP410703 PRJNA907026 SRS15920766 GSM6771441 GSM6771441 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000 SRX18439034 GSM6771442_r1 SRP410703 PRJNA907026 SRS15920767 GSM6771442 GSM6771442 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000 SRX18439035 GSM6771443_r1 SRP410703 PRJNA907026 SRS15920768 GSM6771443 GSM6771443 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000 SRX18439036 GSM6771444_r1 SRP410703 PRJNA907026 SRS15920769 GSM6771444 GSM6771444 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000 SRX18439037 GSM6771445_r1 SRP410703 PRJNA907026 SRS15920770 GSM6771445 GSM6771445 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000 SRX18439038 GSM6771446_r1 SRP410703 PRJNA907026 SRS15920771 GSM6771446 GSM6771446 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000 SRX18439039 GSM6771447_r1 SRP410703 PRJNA907026 SRS15920772 GSM6771447 GSM6771447 OTHER TRANSCRIPTOMIC other Cells were lysed directly in TRIzol followed by isolation of total RNA. The isolated total RNA was further treated with DNAse (TURBO DNA-free Kit; Invitrogen) and a spike-in cocktail of Bru-labeled and unlabeled RNA was mixed in with total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013), but with the following modifications. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. A universal ligation adapter and dual-index, barcoded primers were used for PCR. Size selection for all libraries was via agarose gel excision. Illumina NovaSeq 6000