SRX18287367 GSM6734878_r1 SRP408270 PRJNA902375 SRS15779125 GSM6734878 GSM6734878 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Freshly collected 18g core biopsy of liver allograft tissue obtained 12 months post-transplant from 4 STUDY and 4 SOC living donor liver transplant patients was minced into small pieces and incubated with 1 mg/ml collagenase digestion solution at 37 °C for 8 minutes. The isolated liver cell suspensions were resuspended in 20%∆ FCS, 2mM EDTA, 1XPBS solution. Cells were washed and re-suspended in 4% Ultrapure BSA/1x DPBS Library was performed according to the manufacter's instructions (single cell 3' v3 protocol, 10x Genomics). Briefly, GCs were resuspended in the master mix and loaded together with partitioning oil and gel beads into the chip to generate the gel bead-in-emulsion (GEM). The poly-A RNA from the cell lysate contained in every single GEM was retrotranscripted to cDNA, which contains an Ilumina R1 primer sequence, Unique Molecular Identifier (UMI) and the 10x Barcode. The pooled barcoded cDNA was then cleaned up with Silane DynaBeads, amplified by PCR and the apropiated sized fragments were selected with SPRIselect reagent for subsequent library construction. During the library construction Ilumina R2 primer sequence, paired-end constructs with P5 and P7 sequences and a sample index were added. NextSeq 500 SRX18287368 GSM6734879_r1 SRP408270 PRJNA902375 SRS15779126 GSM6734879 GSM6734879 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Freshly collected 18g core biopsy of liver allograft tissue obtained 12 months post-transplant from 4 STUDY and 4 SOC living donor liver transplant patients was minced into small pieces and incubated with 1 mg/ml collagenase digestion solution at 37 °C for 8 minutes. The isolated liver cell suspensions were resuspended in 20%∆ FCS, 2mM EDTA, 1XPBS solution. Cells were washed and re-suspended in 4% Ultrapure BSA/1x DPBS Library was performed according to the manufacter's instructions (single cell 3' v3 protocol, 10x Genomics). Briefly, GCs were resuspended in the master mix and loaded together with partitioning oil and gel beads into the chip to generate the gel bead-in-emulsion (GEM). The poly-A RNA from the cell lysate contained in every single GEM was retrotranscripted to cDNA, which contains an Ilumina R1 primer sequence, Unique Molecular Identifier (UMI) and the 10x Barcode. The pooled barcoded cDNA was then cleaned up with Silane DynaBeads, amplified by PCR and the apropiated sized fragments were selected with SPRIselect reagent for subsequent library construction. During the library construction Ilumina R2 primer sequence, paired-end constructs with P5 and P7 sequences and a sample index were added. NextSeq 500 SRX18287369 GSM6734880_r1 SRP408270 PRJNA902375 SRS15779127 GSM6734880 GSM6734880 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Freshly collected 18g core biopsy of liver allograft tissue obtained 12 months post-transplant from 4 STUDY and 4 SOC living donor liver transplant patients was minced into small pieces and incubated with 1 mg/ml collagenase digestion solution at 37 °C for 8 minutes. The isolated liver cell suspensions were resuspended in 20%∆ FCS, 2mM EDTA, 1XPBS solution. Cells were washed and re-suspended in 4% Ultrapure BSA/1x DPBS Library was performed according to the manufacter's instructions (single cell 3' v3 protocol, 10x Genomics). Briefly, GCs were resuspended in the master mix and loaded together with partitioning oil and gel beads into the chip to generate the gel bead-in-emulsion (GEM). The poly-A RNA from the cell lysate contained in every single GEM was retrotranscripted to cDNA, which contains an Ilumina R1 primer sequence, Unique Molecular Identifier (UMI) and the 10x Barcode. The pooled barcoded cDNA was then cleaned up with Silane DynaBeads, amplified by PCR and the apropiated sized fragments were selected with SPRIselect reagent for subsequent library construction. During the library construction Ilumina R2 primer sequence, paired-end constructs with P5 and P7 sequences and a sample index were added. NextSeq 500 SRX18287370 GSM6734881_r1 SRP408270 PRJNA902375 SRS15779128 GSM6734881 GSM6734881 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Freshly collected 18g core biopsy of liver allograft tissue obtained 12 months post-transplant from 4 STUDY and 4 SOC living donor liver transplant patients was minced into small pieces and incubated with 1 mg/ml collagenase digestion solution at 37 °C for 8 minutes. The isolated liver cell suspensions were resuspended in 20%∆ FCS, 2mM EDTA, 1XPBS solution. Cells were washed and re-suspended in 4% Ultrapure BSA/1x DPBS Library was performed according to the manufacter's instructions (single cell 3' v3 protocol, 10x Genomics). Briefly, GCs were resuspended in the master mix and loaded together with partitioning oil and gel beads into the chip to generate the gel bead-in-emulsion (GEM). The poly-A RNA from the cell lysate contained in every single GEM was retrotranscripted to cDNA, which contains an Ilumina R1 primer sequence, Unique Molecular Identifier (UMI) and the 10x Barcode. The pooled barcoded cDNA was then cleaned up with Silane DynaBeads, amplified by PCR and the apropiated sized fragments were selected with SPRIselect reagent for subsequent library construction. During the library construction Ilumina R2 primer sequence, paired-end constructs with P5 and P7 sequences and a sample index were added. NextSeq 500 SRX18287371 GSM6734882_r1 SRP408270 PRJNA902375 SRS15779129 GSM6734882 GSM6734882 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Freshly collected 18g core biopsy of liver allograft tissue obtained 12 months post-transplant from 4 STUDY and 4 SOC living donor liver transplant patients was minced into small pieces and incubated with 1 mg/ml collagenase digestion solution at 37 °C for 8 minutes. The isolated liver cell suspensions were resuspended in 20%∆ FCS, 2mM EDTA, 1XPBS solution. Cells were washed and re-suspended in 4% Ultrapure BSA/1x DPBS Library was performed according to the manufacter's instructions (single cell 3' v3 protocol, 10x Genomics). Briefly, GCs were resuspended in the master mix and loaded together with partitioning oil and gel beads into the chip to generate the gel bead-in-emulsion (GEM). The poly-A RNA from the cell lysate contained in every single GEM was retrotranscripted to cDNA, which contains an Ilumina R1 primer sequence, Unique Molecular Identifier (UMI) and the 10x Barcode. The pooled barcoded cDNA was then cleaned up with Silane DynaBeads, amplified by PCR and the apropiated sized fragments were selected with SPRIselect reagent for subsequent library construction. During the library construction Ilumina R2 primer sequence, paired-end constructs with P5 and P7 sequences and a sample index were added. NextSeq 500 SRX18287372 GSM6734883_r1 SRP408270 PRJNA902375 SRS15779130 GSM6734883 GSM6734883 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Freshly collected 18g core biopsy of liver allograft tissue obtained 12 months post-transplant from 4 STUDY and 4 SOC living donor liver transplant patients was minced into small pieces and incubated with 1 mg/ml collagenase digestion solution at 37 °C for 8 minutes. The isolated liver cell suspensions were resuspended in 20%∆ FCS, 2mM EDTA, 1XPBS solution. Cells were washed and re-suspended in 4% Ultrapure BSA/1x DPBS Library was performed according to the manufacter's instructions (single cell 3' v3 protocol, 10x Genomics). Briefly, GCs were resuspended in the master mix and loaded together with partitioning oil and gel beads into the chip to generate the gel bead-in-emulsion (GEM). The poly-A RNA from the cell lysate contained in every single GEM was retrotranscripted to cDNA, which contains an Ilumina R1 primer sequence, Unique Molecular Identifier (UMI) and the 10x Barcode. The pooled barcoded cDNA was then cleaned up with Silane DynaBeads, amplified by PCR and the apropiated sized fragments were selected with SPRIselect reagent for subsequent library construction. During the library construction Ilumina R2 primer sequence, paired-end constructs with P5 and P7 sequences and a sample index were added. NextSeq 500 SRX18287373 GSM6734884_r1 SRP408270 PRJNA902375 SRS15779131 GSM6734884 GSM6734884 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Freshly collected 18g core biopsy of liver allograft tissue obtained 12 months post-transplant from 4 STUDY and 4 SOC living donor liver transplant patients was minced into small pieces and incubated with 1 mg/ml collagenase digestion solution at 37 °C for 8 minutes. The isolated liver cell suspensions were resuspended in 20%∆ FCS, 2mM EDTA, 1XPBS solution. Cells were washed and re-suspended in 4% Ultrapure BSA/1x DPBS Library was performed according to the manufacter's instructions (single cell 3' v3 protocol, 10x Genomics). Briefly, GCs were resuspended in the master mix and loaded together with partitioning oil and gel beads into the chip to generate the gel bead-in-emulsion (GEM). The poly-A RNA from the cell lysate contained in every single GEM was retrotranscripted to cDNA, which contains an Ilumina R1 primer sequence, Unique Molecular Identifier (UMI) and the 10x Barcode. The pooled barcoded cDNA was then cleaned up with Silane DynaBeads, amplified by PCR and the apropiated sized fragments were selected with SPRIselect reagent for subsequent library construction. During the library construction Ilumina R2 primer sequence, paired-end constructs with P5 and P7 sequences and a sample index were added. NextSeq 500 SRX18287374 GSM6734885_r1 SRP408270 PRJNA902375 SRS15779132 GSM6734885 GSM6734885 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Freshly collected 18g core biopsy of liver allograft tissue obtained 12 months post-transplant from 4 STUDY and 4 SOC living donor liver transplant patients was minced into small pieces and incubated with 1 mg/ml collagenase digestion solution at 37 °C for 8 minutes. The isolated liver cell suspensions were resuspended in 20%∆ FCS, 2mM EDTA, 1XPBS solution. Cells were washed and re-suspended in 4% Ultrapure BSA/1x DPBS Library was performed according to the manufacter's instructions (single cell 3' v3 protocol, 10x Genomics). Briefly, GCs were resuspended in the master mix and loaded together with partitioning oil and gel beads into the chip to generate the gel bead-in-emulsion (GEM). The poly-A RNA from the cell lysate contained in every single GEM was retrotranscripted to cDNA, which contains an Ilumina R1 primer sequence, Unique Molecular Identifier (UMI) and the 10x Barcode. The pooled barcoded cDNA was then cleaned up with Silane DynaBeads, amplified by PCR and the apropiated sized fragments were selected with SPRIselect reagent for subsequent library construction. During the library construction Ilumina R2 primer sequence, paired-end constructs with P5 and P7 sequences and a sample index were added. NextSeq 500