SRX18442363 Allagelena_difficilis_JXZ592a SRP410790 bp0 UCE libraries were prepared following: The genomic DNA extracted from each specimen were first fragmentated by sonication (target size of approximately 300–600 bp). The fragmented DNA (21 ul) were then used as input for DNA library preparation with the NEXTFLEX® Rapid DNA-Seq Kit 2.0 (Bioo Scientific) following the manufacturer's protocol with minor modifications. UCE were enriched following the myBaits protocol 5.01 (Arbor Biosciences). The enriched UCE libraries were then sent to Novogene Co. Ltd. for sequencing using the Illumina NovaSeq platform with 150 bp paired-end reads. SRS15923751 Allagelena_difficilis_JXZ592a Allagelena_difficilis_JXZ592a Targeted-Capture GENOMIC Hybrid Selection Illumina NovaSeq 6000