SRX18459501 C08 SRP403790 PRJNA882201 For PacBio sequencing library, 5-10g genomic DNA was sheared into 10-15 Kb fragments using a g-TUBE device. Then library was constructed using the SMRTbell Express Template Preparation Kit 2.0. Briefly, the sheared fragments were through single-strand overhangs removing, DNA damage repair, end-repair,A-tailing, barcoded overhang adapters ligation. The library was quantified using Qubit 3.0 Fluorometer (Invitrogen, Carlsbad, CA), and the size of the library was checked using an Agilent 2100 Bioanalyzer System. Subsequent steps were followed as per the manufactures instruction to prepare SMRTbell library. The library was sequenced using the Sequel II sequencing platform. SRS15493802 SAMN30929146 C08 WGS GENOMIC RANDOM Sequel II