SRX18507765 GSM6793118_r1 SRP411591 PRJNA909179 SRS15976859 GSM6793118 GSM6793118 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA For analyses of infiltrative immune cells, single-cell suspensions (also see Flow cytometry) from APTKA PT, LM and PC samples (cells from 6 mice per location were pooled before) were subjected to single-cell sorting using a MoFlow Astrios machine. After exclusion of CD45- cells, all CD45+ / CD11B- cells were sorted into Eppendorf tubes and directly processed for 10X protocols. Single cell capture, reverse transcription, and library preparation were carried out on the Chromium platform (10X Genomics) with the single-cell 3' reagent v2 protocol according to the manufacturer's recommendations using 50,000 cells as input per reaction well. The final libraries [PT, LM, PC] were pooled and sequenced on two Illumina NovaSeq SP lanes (paired-end 26 bp + 96 bp). Illumina NovaSeq 6000 SRX18507766 GSM6793119_r1 SRP411591 PRJNA909179 SRS15976860 GSM6793119 GSM6793119 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA For analyses of infiltrative immune cells, single-cell suspensions (also see Flow cytometry) from APTKA PT, LM and PC samples (cells from 6 mice per location were pooled before) were subjected to single-cell sorting using a MoFlow Astrios machine. After exclusion of CD45- cells, all CD45+ / CD11B- cells were sorted into Eppendorf tubes and directly processed for 10X protocols. Single cell capture, reverse transcription, and library preparation were carried out on the Chromium platform (10X Genomics) with the single-cell 3' reagent v2 protocol according to the manufacturer's recommendations using 50,000 cells as input per reaction well. The final libraries [PT, LM, PC] were pooled and sequenced on two Illumina NovaSeq SP lanes (paired-end 26 bp + 96 bp). Illumina NovaSeq 6000 SRX18507767 GSM6793120_r1 SRP411591 PRJNA909179 SRS15976861 GSM6793120 GSM6793120 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA For analyses of infiltrative immune cells, single-cell suspensions (also see Flow cytometry) from APTKA PT, LM and PC samples (cells from 6 mice per location were pooled before) were subjected to single-cell sorting using a MoFlow Astrios machine. After exclusion of CD45- cells, all CD45+ / CD11B- cells were sorted into Eppendorf tubes and directly processed for 10X protocols. Single cell capture, reverse transcription, and library preparation were carried out on the Chromium platform (10X Genomics) with the single-cell 3' reagent v2 protocol according to the manufacturer's recommendations using 50,000 cells as input per reaction well. The final libraries [PT, LM, PC] were pooled and sequenced on two Illumina NovaSeq SP lanes (paired-end 26 bp + 96 bp). Illumina NovaSeq 6000