SRX18535757 WFX1997-1 SRP411921 bp0 mRNA was purified from total RNA by using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in First Strand Synthesis Reaction Buffer(5X). First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase, then use RNaseH to degrade the RNA. Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and dNTP. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3 ends of DNA fragments, Adaptor with hairpin loop structure were ligated to prepare for hybridization SRS16000349 SAMN32082921 WFX1997-1 RNA-Seq TRANSCRIPTOMIC RT-PCR Illumina NovaSeq 6000 SRX18535758 WFX1997-2 SRP411921 bp0 mRNA was purified from total RNA by using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in First Strand Synthesis Reaction Buffer(5X). First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase, then use RNaseH to degrade the RNA. Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and dNTP. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 4 ends of DNA fragments, Adaptor with hairpin loop structure were ligated to prepare for hybridization SRS16000350 SAMN32082922 WFX1997-2 RNA-Seq TRANSCRIPTOMIC RT-PCR Illumina NovaSeq 6000 SRX18535759 WFX1997-11 SRP411921 bp0 mRNA was purified from total RNA by using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in First Strand Synthesis Reaction Buffer(5X). First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase, then use RNaseH to degrade the RNA. Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and dNTP. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 13 ends of DNA fragments, Adaptor with hairpin loop structure were ligated to prepare for hybridization SRS16000351 SAMN32082931 WFX1997-11 RNA-Seq TRANSCRIPTOMIC RT-PCR Illumina NovaSeq 6000 SRX18535760 WFX1997-12 SRP411921 bp0 mRNA was purified from total RNA by using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in First Strand Synthesis Reaction Buffer(5X). First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase, then use RNaseH to degrade the RNA. Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and dNTP. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 14 ends of DNA fragments, Adaptor with hairpin loop structure were ligated to prepare for hybridization SRS16000352 SAMN32082932 WFX1997-12 RNA-Seq TRANSCRIPTOMIC RT-PCR Illumina NovaSeq 6000 SRX18535761 WFX1997-3 SRP411921 bp0 mRNA was purified from total RNA by using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in First Strand Synthesis Reaction Buffer(5X). First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase, then use RNaseH to degrade the RNA. Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and dNTP. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 5 ends of DNA fragments, Adaptor with hairpin loop structure were ligated to prepare for hybridization SRS16000353 SAMN32082923 WFX1997-3 RNA-Seq TRANSCRIPTOMIC RT-PCR Illumina NovaSeq 6000 SRX18535762 WFX1997-4 SRP411921 bp0 mRNA was purified from total RNA by using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in First Strand Synthesis Reaction Buffer(5X). First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase, then use RNaseH to degrade the RNA. Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and dNTP. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 6 ends of DNA fragments, Adaptor with hairpin loop structure were ligated to prepare for hybridization SRS16000354 SAMN32082924 WFX1997-4 RNA-Seq TRANSCRIPTOMIC RT-PCR Illumina NovaSeq 6000 SRX18535763 WFX1997-5 SRP411921 bp0 mRNA was purified from total RNA by using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in First Strand Synthesis Reaction Buffer(5X). First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase, then use RNaseH to degrade the RNA. Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and dNTP. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 7 ends of DNA fragments, Adaptor with hairpin loop structure were ligated to prepare for hybridization SRS16000355 SAMN32082925 WFX1997-5 RNA-Seq TRANSCRIPTOMIC RT-PCR Illumina NovaSeq 6000 SRX18535764 WFX1997-6 SRP411921 bp0 mRNA was purified from total RNA by using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in First Strand Synthesis Reaction Buffer(5X). First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase, then use RNaseH to degrade the RNA. Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and dNTP. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 8 ends of DNA fragments, Adaptor with hairpin loop structure were ligated to prepare for hybridization SRS16000356 SAMN32082926 WFX1997-6 RNA-Seq TRANSCRIPTOMIC RT-PCR Illumina NovaSeq 6000 SRX18535765 WFX1997-7 SRP411921 bp0 mRNA was purified from total RNA by using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in First Strand Synthesis Reaction Buffer(5X). First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase, then use RNaseH to degrade the RNA. Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and dNTP. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 9 ends of DNA fragments, Adaptor with hairpin loop structure were ligated to prepare for hybridization SRS16000357 SAMN32082927 WFX1997-7 RNA-Seq TRANSCRIPTOMIC RT-PCR Illumina NovaSeq 6000 SRX18535766 WFX1997-8 SRP411921 bp0 mRNA was purified from total RNA by using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in First Strand Synthesis Reaction Buffer(5X). First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase, then use RNaseH to degrade the RNA. Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and dNTP. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 10 ends of DNA fragments, Adaptor with hairpin loop structure were ligated to prepare for hybridization SRS16000358 SAMN32082928 WFX1997-8 RNA-Seq TRANSCRIPTOMIC RT-PCR Illumina NovaSeq 6000 SRX18535767 WFX1997-9 SRP411921 bp0 mRNA was purified from total RNA by using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in First Strand Synthesis Reaction Buffer(5X). First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase, then use RNaseH to degrade the RNA. Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and dNTP. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 11 ends of DNA fragments, Adaptor with hairpin loop structure were ligated to prepare for hybridization SRS16000359 SAMN32082929 WFX1997-9 RNA-Seq TRANSCRIPTOMIC RT-PCR Illumina NovaSeq 6000 SRX18535768 WFX1997-10 SRP411921 bp0 mRNA was purified from total RNA by using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in First Strand Synthesis Reaction Buffer(5X). First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase, then use RNaseH to degrade the RNA. Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and dNTP. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 12 ends of DNA fragments, Adaptor with hairpin loop structure were ligated to prepare for hybridization SRS16000360 SAMN32082930 WFX1997-10 RNA-Seq TRANSCRIPTOMIC RT-PCR Illumina NovaSeq 6000