SRX18538731 GSM6806577_r1 SRP412007 PRJNA910143 SRS16003121 GSM6806577 GSM6806577 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Cells were lysed in sealed microchambers and then mRNA was captured by barcoded microbeads cDNA production occurs in the sealed micro chambers through action of reverse transcriptase. cDNA amplification protocol was developed in-house using a commercially available polymerase. Illumina library preparation was done using the manufacturer's protocol and Nextera XD library preparation kit (Illumina, FC-131-1096). NextSeq 1000