SRX18556351 Human Sputum EM60B SRP412233 bp0 Samples were preserved immediately in RNA-later and stored at -80C. Samples were thawed, RNA-later was removed, and samples were resuspended in RNase-free TE buffer containing lysozyme and lysostaphin. Samples were incubated for 30 min at 37C for enzymatic lysis, RNA-Bee was added, and samples were bead beat 3X for 30 s placing on ice in between each round. Chloroform was added to each sample, samples were mixed and centrifuged to separate phases. The aqueous phase was removed to a new tube and the RNA was precipitated with isopropanol. Ribosomal RNA was depleted with an Illumina RiboZero Gold (epidemiology) kit and RNA was fragmented with the NEBNext Magnesium fragmentation module per the manufacturer's instructions. Sequencing libraries were prepared with the NEBNext Small RNA Library prep kit, and adapter dimers were removed by size selection on a 5% TBE polyacrylamide gel. SRS16019403 HumanSputum_EM60B Human Sputum EM60B RNA-Seq TRANSCRIPTOMIC RANDOM NextSeq 500 SRX18556353 Human Sputum EM61 SRP412233 bp0 Samples were preserved immediately in RNA-later and stored at -80C. Samples were thawed, RNA-later was removed, and samples were resuspended in RNase-free TE buffer containing lysozyme and lysostaphin. Samples were incubated for 30 min at 37C for enzymatic lysis, RNA-Bee was added, and samples were bead beat 3X for 30 s placing on ice in between each round. Chloroform was added to each sample, samples were mixed and centrifuged to separate phases. The aqueous phase was removed to a new tube and the RNA was precipitated with isopropanol. Ribosomal RNA was depleted with an Illumina RiboZero Gold (epidemiology) kit and RNA was fragmented with the NEBNext Magnesium fragmentation module per the manufacturer's instructions. Sequencing libraries were prepared with the NEBNext Small RNA Library prep kit, and adapter dimers were removed by size selection on a 5% TBE polyacrylamide gel. SRS16019404 HumanSputum_EM61 Human Sputum EM61 RNA-Seq TRANSCRIPTOMIC RANDOM NextSeq 500 SRX18556355 Human Sputum EM59 SRP412233 bp0 Samples were preserved immediately in RNA-later and stored at -80C. Samples were thawed, RNA-later was removed, and samples were resuspended in RNase-free TE buffer containing lysozyme and lysostaphin. Samples were incubated for 30 min at 37C for enzymatic lysis, RNA-Bee was added, and samples were bead beat 3X for 30 s placing on ice in between each round. Chloroform was added to each sample, samples were mixed and centrifuged to separate phases. The aqueous phase was removed to a new tube and the RNA was precipitated with isopropanol. Ribosomal RNA was depleted with an Illumina RiboZero Gold (epidemiology) kit and RNA was fragmented with the NEBNext Magnesium fragmentation module per the manufacturer's instructions. Sequencing libraries were prepared with the NEBNext Small RNA Library prep kit, and adapter dimers were removed by size selection on a 5% TBE polyacrylamide gel. SRS16019405 HumanSputum_EM59 Human Sputum EM59 RNA-Seq TRANSCRIPTOMIC RANDOM NextSeq 500 SRX18556357 Human Sputum EM70 SRP412233 bp0 Samples were preserved immediately in RNA-later and stored at -80C. Samples were thawed, RNA-later was removed, and samples were resuspended in RNase-free TE buffer containing lysozyme and lysostaphin. Samples were incubated for 30 min at 37C for enzymatic lysis, RNA-bee was added, and samples were bead beat 3X for 30 s placing on ice in between each round. Chloroform was added to each sample, samples were mixed and centrifuged to separate phases. The aqueous phase was removed to a new tube and the RNA was precipitated with isopropanol. Ribosomal RNA was depleted with the NEBNext rRNA Depletion Kit with Human/Mouse/Rat and Bacterial probes mixed 1:1. Sequencing libraries were prepared with the NEBNext Small RNA Library prep kit, and adapter dimers were removed by size selection on a 5% TBE polyacrylamide gel. SRS16019406 HumanSputum_EM70 Human Sputum EM70 RNA-Seq TRANSCRIPTOMIC RANDOM NextSeq 500 SRX18556359 Human Sputum EM55B SRP412233 bp0 Samples were preserved immediately in RNA-later and stored at -80C. Samples were thawed, RNA-later was removed, and samples were resuspended in RNase-free TE buffer containing lysozyme and lysostaphin. Samples were incubated for 30 min at 37C for enzymatic lysis, RNA-Bee was added, and samples were bead beat 3X for 30 s placing on ice in between each round. Chloroform was added to each sample, samples were mixed and centrifuged to separate phases. The aqueous phase was removed to a new tube and the RNA was precipitated with isopropanol. Ribosomal RNA was depleted with an Illumina RiboZero Gold (epidemiology) kit and RNA was fragmented with the NEBNext Magnesium fragmentation module per the manufacturer's instructions. Sequencing libraries were prepared with the NEBNext Small RNA Library prep kit, and adapter dimers were removed by size selection on a 5% TBE polyacrylamide gel. SRS16019407 HumanSputum_EM55B Human Sputum EM55B RNA-Seq TRANSCRIPTOMIC RANDOM NextSeq 500