SRX18552193 GSM6807935_r1 SRP412226 PRJNA910552 SRS16018424 GSM6807935 GSM6807935 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Single-cell RNAseq libraries prepared using 10x protocol from eye organoids derived from DRAM2 WT (1 sample) and DRAM2 KO stem cells (1 sample). N=2 samples. Library was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics). Briefly, GCs were resuspended in the master mix and loaded together with partitioning oil and gel beads into the chip to generate the gel bead-in-emulsion (GEM). The poly-A RNA from the cell lysate contained in every single GEM was retrotranscripted to cDNA, which contains an Ilumina R1 primer sequence, Unique Molecular Identifier (UMI) and the 10x Barcode. The pooled barcoded cDNA was then cleaned up with Silane DynaBeads, amplified by PCR and the apropiated sized fragments were selected with SPRIselect reagent for subsequent library construction. During the library construction Ilumina R2 primer sequence, paired-end constructs with P5 and P7 sequences and a sample index were added. Illumina HiSeq 4000 SRX18552194 GSM6807936_r1 SRP412226 PRJNA910552 SRS16018425 GSM6807936 GSM6807936 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Single-cell RNAseq libraries prepared using 10x protocol from eye organoids derived from DRAM2 WT (1 sample) and DRAM2 KO stem cells (1 sample). N=2 samples. Library was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics). Briefly, GCs were resuspended in the master mix and loaded together with partitioning oil and gel beads into the chip to generate the gel bead-in-emulsion (GEM). The poly-A RNA from the cell lysate contained in every single GEM was retrotranscripted to cDNA, which contains an Ilumina R1 primer sequence, Unique Molecular Identifier (UMI) and the 10x Barcode. The pooled barcoded cDNA was then cleaned up with Silane DynaBeads, amplified by PCR and the apropiated sized fragments were selected with SPRIselect reagent for subsequent library construction. During the library construction Ilumina R2 primer sequence, paired-end constructs with P5 and P7 sequences and a sample index were added. Illumina HiSeq 4000 SRX18552195 GSM6807937_r1 SRP412226 PRJNA910552 SRS16018426 GSM6807937 GSM6807937 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Single-cell RNAseq libraries prepared using 10x protocol from eye organoids derived from DRAM2 WT (1 sample) and DRAM2 KO stem cells (1 sample). N=2 samples. Library was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics). Briefly, GCs were resuspended in the master mix and loaded together with partitioning oil and gel beads into the chip to generate the gel bead-in-emulsion (GEM). The poly-A RNA from the cell lysate contained in every single GEM was retrotranscripted to cDNA, which contains an Ilumina R1 primer sequence, Unique Molecular Identifier (UMI) and the 10x Barcode. The pooled barcoded cDNA was then cleaned up with Silane DynaBeads, amplified by PCR and the apropiated sized fragments were selected with SPRIselect reagent for subsequent library construction. During the library construction Ilumina R2 primer sequence, paired-end constructs with P5 and P7 sequences and a sample index were added. Illumina HiSeq 4000 SRX18552196 GSM6807938_r1 SRP412226 PRJNA910552 SRS16018427 GSM6807938 GSM6807938 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Single-cell RNAseq libraries prepared using 10x protocol from eye organoids derived from DRAM2 WT (1 sample) and DRAM2 KO stem cells (1 sample). N=2 samples. Library was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics). Briefly, GCs were resuspended in the master mix and loaded together with partitioning oil and gel beads into the chip to generate the gel bead-in-emulsion (GEM). The poly-A RNA from the cell lysate contained in every single GEM was retrotranscripted to cDNA, which contains an Ilumina R1 primer sequence, Unique Molecular Identifier (UMI) and the 10x Barcode. The pooled barcoded cDNA was then cleaned up with Silane DynaBeads, amplified by PCR and the apropiated sized fragments were selected with SPRIselect reagent for subsequent library construction. During the library construction Ilumina R2 primer sequence, paired-end constructs with P5 and P7 sequences and a sample index were added. Illumina HiSeq 4000 SRX18552197 GSM6807939_r1 SRP412226 PRJNA910552 SRS16018428 GSM6807939 GSM6807939 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Single-cell RNAseq libraries prepared using 10x protocol from eye organoids derived from DRAM2 WT (1 sample) and DRAM2 KO stem cells (1 sample). N=2 samples. Library was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics). Briefly, GCs were resuspended in the master mix and loaded together with partitioning oil and gel beads into the chip to generate the gel bead-in-emulsion (GEM). The poly-A RNA from the cell lysate contained in every single GEM was retrotranscripted to cDNA, which contains an Ilumina R1 primer sequence, Unique Molecular Identifier (UMI) and the 10x Barcode. The pooled barcoded cDNA was then cleaned up with Silane DynaBeads, amplified by PCR and the apropiated sized fragments were selected with SPRIselect reagent for subsequent library construction. During the library construction Ilumina R2 primer sequence, paired-end constructs with P5 and P7 sequences and a sample index were added. Illumina HiSeq 4000 SRX18552198 GSM6807940_r1 SRP412226 PRJNA910552 SRS16018429 GSM6807940 GSM6807940 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Single-cell RNAseq libraries prepared using 10x protocol from eye organoids derived from DRAM2 WT (1 sample) and DRAM2 KO stem cells (1 sample). N=2 samples. Library was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics). Briefly, GCs were resuspended in the master mix and loaded together with partitioning oil and gel beads into the chip to generate the gel bead-in-emulsion (GEM). The poly-A RNA from the cell lysate contained in every single GEM was retrotranscripted to cDNA, which contains an Ilumina R1 primer sequence, Unique Molecular Identifier (UMI) and the 10x Barcode. The pooled barcoded cDNA was then cleaned up with Silane DynaBeads, amplified by PCR and the apropiated sized fragments were selected with SPRIselect reagent for subsequent library construction. During the library construction Ilumina R2 primer sequence, paired-end constructs with P5 and P7 sequences and a sample index were added. Illumina HiSeq 4000 SRX18552199 GSM6807941_r1 SRP412226 PRJNA910552 SRS16018430 GSM6807941 GSM6807941 RNA-Seq TRANSCRIPTOMIC SINGLE CELL cDNA Single-cell RNAseq libraries prepared using 10x protocol from eye organoids derived from DRAM2 WT (1 sample) and DRAM2 KO stem cells (1 sample). N=2 samples. Library was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics). Briefly, GCs were resuspended in the master mix and loaded together with partitioning oil and gel beads into the chip to generate the gel bead-in-emulsion (GEM). The poly-A RNA from the cell lysate contained in every single GEM was retrotranscripted to cDNA, which contains an Ilumina R1 primer sequence, Unique Molecular Identifier (UMI) and the 10x Barcode. The pooled barcoded cDNA was then cleaned up with Silane DynaBeads, amplified by PCR and the apropiated sized fragments were selected with SPRIselect reagent for subsequent library construction. During the library construction Ilumina R2 primer sequence, paired-end constructs with P5 and P7 sequences and a sample index were added. Illumina HiSeq 4000