SRX18653116 GSM6816330_r1 SRP412585 PRJNA911290 SRS16097042 GSM6816330 GSM6816330 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was purified using mRNeasy kit (Qiagen) and eluted in 50 uL of EB (Qiagen) polyadenylated mRNAs were enriched using Oligo dT selection, followed by cDNA synthesis using random N6-primed reverse transcription and a second-strand cDNA synthesis with dUTP instead of dTTP. The synthesised cDNA was then subjected to end-repair and 3' adenylated, followed by adaptor ligation to both ends. The dUTP-marked strand was selectively degraded using Uracil-DNA-Glycosylase (UDG). The remaining strand was amplified to generate a cDNA library suitable for sequencing prior to several cyclyes of PCR amplifcation. The PCR products were heat-denatured and the single strand DNA was cyclised by splint oligos and DNA ligase, followed by DNA nanoball synthesis and sequencing on DNBSEQ platform (BGI) DNBSEQ-G400 SRX18653117 GSM6816331_r1 SRP412585 PRJNA911290 SRS16097043 GSM6816331 GSM6816331 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was purified using mRNeasy kit (Qiagen) and eluted in 50 uL of EB (Qiagen) polyadenylated mRNAs were enriched using Oligo dT selection, followed by cDNA synthesis using random N6-primed reverse transcription and a second-strand cDNA synthesis with dUTP instead of dTTP. The synthesised cDNA was then subjected to end-repair and 3' adenylated, followed by adaptor ligation to both ends. The dUTP-marked strand was selectively degraded using Uracil-DNA-Glycosylase (UDG). The remaining strand was amplified to generate a cDNA library suitable for sequencing prior to several cyclyes of PCR amplifcation. The PCR products were heat-denatured and the single strand DNA was cyclised by splint oligos and DNA ligase, followed by DNA nanoball synthesis and sequencing on DNBSEQ platform (BGI) DNBSEQ-G400 SRX18653118 GSM6816332_r1 SRP412585 PRJNA911290 SRS16097044 GSM6816332 GSM6816332 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was purified using mRNeasy kit (Qiagen) and eluted in 50 uL of EB (Qiagen) polyadenylated mRNAs were enriched using Oligo dT selection, followed by cDNA synthesis using random N6-primed reverse transcription and a second-strand cDNA synthesis with dUTP instead of dTTP. The synthesised cDNA was then subjected to end-repair and 3' adenylated, followed by adaptor ligation to both ends. The dUTP-marked strand was selectively degraded using Uracil-DNA-Glycosylase (UDG). The remaining strand was amplified to generate a cDNA library suitable for sequencing prior to several cyclyes of PCR amplifcation. The PCR products were heat-denatured and the single strand DNA was cyclised by splint oligos and DNA ligase, followed by DNA nanoball synthesis and sequencing on DNBSEQ platform (BGI) DNBSEQ-G400 SRX18653119 GSM6816333_r1 SRP412585 PRJNA911290 SRS16097045 GSM6816333 GSM6816333 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was purified using mRNeasy kit (Qiagen) and eluted in 50 uL of EB (Qiagen) polyadenylated mRNAs were enriched using Oligo dT selection, followed by cDNA synthesis using random N6-primed reverse transcription and a second-strand cDNA synthesis with dUTP instead of dTTP. The synthesised cDNA was then subjected to end-repair and 3' adenylated, followed by adaptor ligation to both ends. The dUTP-marked strand was selectively degraded using Uracil-DNA-Glycosylase (UDG). The remaining strand was amplified to generate a cDNA library suitable for sequencing prior to several cyclyes of PCR amplifcation. The PCR products were heat-denatured and the single strand DNA was cyclised by splint oligos and DNA ligase, followed by DNA nanoball synthesis and sequencing on DNBSEQ platform (BGI) DNBSEQ-G400 SRX18653120 GSM6816334_r1 SRP412585 PRJNA911290 SRS16097046 GSM6816334 GSM6816334 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was purified using mRNeasy kit (Qiagen) and eluted in 50 uL of EB (Qiagen) polyadenylated mRNAs were enriched using Oligo dT selection, followed by cDNA synthesis using random N6-primed reverse transcription and a second-strand cDNA synthesis with dUTP instead of dTTP. The synthesised cDNA was then subjected to end-repair and 3' adenylated, followed by adaptor ligation to both ends. The dUTP-marked strand was selectively degraded using Uracil-DNA-Glycosylase (UDG). The remaining strand was amplified to generate a cDNA library suitable for sequencing prior to several cyclyes of PCR amplifcation. The PCR products were heat-denatured and the single strand DNA was cyclised by splint oligos and DNA ligase, followed by DNA nanoball synthesis and sequencing on DNBSEQ platform (BGI) DNBSEQ-G400 SRX18653121 GSM6816336_r1 SRP412585 PRJNA911290 SRS16097047 GSM6816336 GSM6816336 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was purified using mRNeasy kit (Qiagen) and eluted in 50 uL of EB (Qiagen) polyadenylated mRNAs were enriched using Oligo dT selection, followed by cDNA synthesis using random N6-primed reverse transcription and a second-strand cDNA synthesis with dUTP instead of dTTP. The synthesised cDNA was then subjected to end-repair and 3' adenylated, followed by adaptor ligation to both ends. The dUTP-marked strand was selectively degraded using Uracil-DNA-Glycosylase (UDG). The remaining strand was amplified to generate a cDNA library suitable for sequencing prior to several cyclyes of PCR amplifcation. The PCR products were heat-denatured and the single strand DNA was cyclised by splint oligos and DNA ligase, followed by DNA nanoball synthesis and sequencing on DNBSEQ platform (BGI) DNBSEQ-G400 SRX18653122 GSM6816337_r1 SRP412585 PRJNA911290 SRS16097048 GSM6816337 GSM6816337 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was purified using mRNeasy kit (Qiagen) and eluted in 50 uL of EB (Qiagen) polyadenylated mRNAs were enriched using Oligo dT selection, followed by cDNA synthesis using random N6-primed reverse transcription and a second-strand cDNA synthesis with dUTP instead of dTTP. The synthesised cDNA was then subjected to end-repair and 3' adenylated, followed by adaptor ligation to both ends. The dUTP-marked strand was selectively degraded using Uracil-DNA-Glycosylase (UDG). The remaining strand was amplified to generate a cDNA library suitable for sequencing prior to several cyclyes of PCR amplifcation. The PCR products were heat-denatured and the single strand DNA was cyclised by splint oligos and DNA ligase, followed by DNA nanoball synthesis and sequencing on DNBSEQ platform (BGI) DNBSEQ-G400 SRX18653123 GSM6816338_r1 SRP412585 PRJNA911290 SRS16097049 GSM6816338 GSM6816338 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was purified using mRNeasy kit (Qiagen) and eluted in 50 uL of EB (Qiagen) polyadenylated mRNAs were enriched using Oligo dT selection, followed by cDNA synthesis using random N6-primed reverse transcription and a second-strand cDNA synthesis with dUTP instead of dTTP. The synthesised cDNA was then subjected to end-repair and 3' adenylated, followed by adaptor ligation to both ends. The dUTP-marked strand was selectively degraded using Uracil-DNA-Glycosylase (UDG). The remaining strand was amplified to generate a cDNA library suitable for sequencing prior to several cyclyes of PCR amplifcation. The PCR products were heat-denatured and the single strand DNA was cyclised by splint oligos and DNA ligase, followed by DNA nanoball synthesis and sequencing on DNBSEQ platform (BGI) DNBSEQ-G400 SRX18653124 GSM6816339_r1 SRP412585 PRJNA911290 SRS16097050 GSM6816339 GSM6816339 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was purified using mRNeasy kit (Qiagen) and eluted in 50 uL of EB (Qiagen) polyadenylated mRNAs were enriched using Oligo dT selection, followed by cDNA synthesis using random N6-primed reverse transcription and a second-strand cDNA synthesis with dUTP instead of dTTP. The synthesised cDNA was then subjected to end-repair and 3' adenylated, followed by adaptor ligation to both ends. The dUTP-marked strand was selectively degraded using Uracil-DNA-Glycosylase (UDG). The remaining strand was amplified to generate a cDNA library suitable for sequencing prior to several cyclyes of PCR amplifcation. The PCR products were heat-denatured and the single strand DNA was cyclised by splint oligos and DNA ligase, followed by DNA nanoball synthesis and sequencing on DNBSEQ platform (BGI) DNBSEQ-G400