SRX18690962 GSM6829534_r1 SRP412978 PRJNA912100 SRS16133705 GSM6829534 GSM6829534 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from sorted cells with a RNeasy plus mini kit (Qiagen) according to the manufacturer's instructions. The quantity and quality of the isolated RNA was determined with a Qubit (1.0) Fluorometer (Life Technologies) and a 4200 TapeStation System (Agilent). Libraries were prepared following the Takara SMART-Seq v4 Ultra Low Input RNA Kit, with PolyA selection. For the CD27SP NK cell isolated from the BM, after quantification, libraries were prepared for loading accordingly to the NovaSeq workflow with the NovaSeq6000 Reagent Kit (Illumina). Cluster generation and sequencing were performed on a NovaSeq6000 System with a run configuration of single end 100bp with a depth of around 20 Mio reads per sample. For the splenic mature NK cell, the TruSeq SR Cluster Kit HS40t00 (Illumina) was used for cluster generation of pooled normalized libraries on the cBOT. Sequencing was performed on the Illumina HiSeq 4000 single end 125 bp using the TruSeq SBS Kit HS4000 (Illumina) with a depth of around 20 Mio reads per sample. Illumina HiSeq 4000 SRX18690963 GSM6829535_r1 SRP412978 PRJNA912100 SRS16133706 GSM6829535 GSM6829535 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from sorted cells with a RNeasy plus mini kit (Qiagen) according to the manufacturer's instructions. The quantity and quality of the isolated RNA was determined with a Qubit (1.0) Fluorometer (Life Technologies) and a 4200 TapeStation System (Agilent). Libraries were prepared following the Takara SMART-Seq v4 Ultra Low Input RNA Kit, with PolyA selection. For the CD27SP NK cell isolated from the BM, after quantification, libraries were prepared for loading accordingly to the NovaSeq workflow with the NovaSeq6000 Reagent Kit (Illumina). Cluster generation and sequencing were performed on a NovaSeq6000 System with a run configuration of single end 100bp with a depth of around 20 Mio reads per sample. For the splenic mature NK cell, the TruSeq SR Cluster Kit HS40t00 (Illumina) was used for cluster generation of pooled normalized libraries on the cBOT. Sequencing was performed on the Illumina HiSeq 4000 single end 125 bp using the TruSeq SBS Kit HS4000 (Illumina) with a depth of around 20 Mio reads per sample. Illumina HiSeq 4000 SRX18690964 GSM6829536_r1 SRP412978 PRJNA912100 SRS16133707 GSM6829536 GSM6829536 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from sorted cells with a RNeasy plus mini kit (Qiagen) according to the manufacturer's instructions. The quantity and quality of the isolated RNA was determined with a Qubit (1.0) Fluorometer (Life Technologies) and a 4200 TapeStation System (Agilent). Libraries were prepared following the Takara SMART-Seq v4 Ultra Low Input RNA Kit, with PolyA selection. For the CD27SP NK cell isolated from the BM, after quantification, libraries were prepared for loading accordingly to the NovaSeq workflow with the NovaSeq6000 Reagent Kit (Illumina). Cluster generation and sequencing were performed on a NovaSeq6000 System with a run configuration of single end 100bp with a depth of around 20 Mio reads per sample. For the splenic mature NK cell, the TruSeq SR Cluster Kit HS40t00 (Illumina) was used for cluster generation of pooled normalized libraries on the cBOT. Sequencing was performed on the Illumina HiSeq 4000 single end 125 bp using the TruSeq SBS Kit HS4000 (Illumina) with a depth of around 20 Mio reads per sample. Illumina HiSeq 4000 SRX18690965 GSM6829537_r1 SRP412978 PRJNA912100 SRS16133708 GSM6829537 GSM6829537 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from sorted cells with a RNeasy plus mini kit (Qiagen) according to the manufacturer's instructions. The quantity and quality of the isolated RNA was determined with a Qubit (1.0) Fluorometer (Life Technologies) and a 4200 TapeStation System (Agilent). Libraries were prepared following the Takara SMART-Seq v4 Ultra Low Input RNA Kit, with PolyA selection. For the CD27SP NK cell isolated from the BM, after quantification, libraries were prepared for loading accordingly to the NovaSeq workflow with the NovaSeq6000 Reagent Kit (Illumina). Cluster generation and sequencing were performed on a NovaSeq6000 System with a run configuration of single end 100bp with a depth of around 20 Mio reads per sample. For the splenic mature NK cell, the TruSeq SR Cluster Kit HS40t00 (Illumina) was used for cluster generation of pooled normalized libraries on the cBOT. Sequencing was performed on the Illumina HiSeq 4000 single end 125 bp using the TruSeq SBS Kit HS4000 (Illumina) with a depth of around 20 Mio reads per sample. Illumina HiSeq 4000 SRX18690966 GSM6829538_r1 SRP412978 PRJNA912100 SRS16133709 GSM6829538 GSM6829538 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from sorted cells with a RNeasy plus mini kit (Qiagen) according to the manufacturer's instructions. The quantity and quality of the isolated RNA was determined with a Qubit (1.0) Fluorometer (Life Technologies) and a 4200 TapeStation System (Agilent). Libraries were prepared following the Takara SMART-Seq v4 Ultra Low Input RNA Kit, with PolyA selection. For the CD27SP NK cell isolated from the BM, after quantification, libraries were prepared for loading accordingly to the NovaSeq workflow with the NovaSeq6000 Reagent Kit (Illumina). Cluster generation and sequencing were performed on a NovaSeq6000 System with a run configuration of single end 100bp with a depth of around 20 Mio reads per sample. For the splenic mature NK cell, the TruSeq SR Cluster Kit HS40t00 (Illumina) was used for cluster generation of pooled normalized libraries on the cBOT. Sequencing was performed on the Illumina HiSeq 4000 single end 125 bp using the TruSeq SBS Kit HS4000 (Illumina) with a depth of around 20 Mio reads per sample. Illumina HiSeq 4000 SRX18690967 GSM6829539_r1 SRP412978 PRJNA912100 SRS16133710 GSM6829539 GSM6829539 RNA-Seq TRANSCRIPTOMIC cDNA Total RNA was extracted from sorted cells with a RNeasy plus mini kit (Qiagen) according to the manufacturer's instructions. The quantity and quality of the isolated RNA was determined with a Qubit (1.0) Fluorometer (Life Technologies) and a 4200 TapeStation System (Agilent). Libraries were prepared following the Takara SMART-Seq v4 Ultra Low Input RNA Kit, with PolyA selection. For the CD27SP NK cell isolated from the BM, after quantification, libraries were prepared for loading accordingly to the NovaSeq workflow with the NovaSeq6000 Reagent Kit (Illumina). Cluster generation and sequencing were performed on a NovaSeq6000 System with a run configuration of single end 100bp with a depth of around 20 Mio reads per sample. For the splenic mature NK cell, the TruSeq SR Cluster Kit HS40t00 (Illumina) was used for cluster generation of pooled normalized libraries on the cBOT. Sequencing was performed on the Illumina HiSeq 4000 single end 125 bp using the TruSeq SBS Kit HS4000 (Illumina) with a depth of around 20 Mio reads per sample. Illumina HiSeq 4000